Establishment of a real-time PCR method for quantification of geosmin-producing Streptomyces spp. in recirculating aquaculture systems

Auffret, Marc; Pilote, Alexandre; Proulx, Émilie; Proulx, Daniel; Vandenberg, Grant W.; Villemur, Richard

doi:10.1016/j.watres.2011.10.020

Cited by 51 publications

(66 citation statements)

References 36 publications

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“…Considering the volume of samples collected, the limit of quantification (LOQ) of these qPCRs should be at the level of 10 3 copies L −1 in field, and this LOQ was verified in the practical monitoring of the three waters. Unlike the qPCR assays targeting geosmin-producing Streptomyces (Auffret et al 2011) and Anabaena (Su et al 2013;Tsao et al 2014) based on SYBR Green, the qPCR methods developed in this study introduced TaqMan technique, which has a high specificity (Savichtcheva et al 2011;Srivastava et al 2013), to quantify odor-producing organisms. To our knowledge, this study is the first report of qPCR assays to detect and quantify potential MIB-producing cyanobacteria.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have revealed that filamentous cyanobacteria species are the main biological source of odorous MIB and geosmin aquatic ecosystems, especially in eutrophication waters (Izaguirre and Taylor 2004;Jüttner and Watson 2007;Smith et al 2008;Watson et al 2008), and actinomyces (mainly as Streptomyces) are usually found as dominant geosmin-producing organisms in the sediment or recirculating aquaculture systems (Zuo et al 2010;Auffret et al 2011). This study indicated that cyanobacterial mic genes were detected and quantified in most investigated water samples, and MIB-producing Streptomyces were identified scarcely, thereby confirmingthat MIB is mainly cyanobacteria originated in Lushui Reservoir, Donghu Lake, and Qinghai Lake.…”

Section: Discussionmentioning

confidence: 99%

“…Taste and odor (T&O) episodes caused by 2-methylisoborneol (MIB) and geosmin (trans-1,10-dimethyl-trans-9-decalol) have been reported worldwide in diverse waters, such as lakes, fish ponds, and reservoirs (Jüttner and Watson 2007;Li et al 2007;Watson et al 2007Watson et al , 2008Auffret et al 2011;Su et al 2013;. These two musty-earthy monoterpene and sesquiterpene metabolites are exceptionally sensitive for humans and have a low sensory threshold of 2 to 10 ng L −1 (Cook et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…To prevent the problems associated with T&O events in drinking water catchments, sensitive and effective methods for the detection of MIB represent a valuable tool (Watson et al 2008;Dzialowski et al 2009). A wide variety of microorganisms, including cyanobacteria, actinomycetes, fungi, and myxobacteria, can synthesize and secrete MIB as a secondary metabolite (Jüttner and Watson 2007;Komatsu et al 2008;Auffret et al 2011), but cyanobacteria species are regarded as the main MIB producers in freshwater ecosystems where photosynthetic growth occurs (Watson 2004;Jüttner and Watson 2007). Thus, the detection and quantification of MIB genes in cyanobacteria could be a valuable indicator for the development of T&O events in natural waters.…”

Section: Introductionmentioning

confidence: 99%

“…The typical advantages of qPCR, including sensitivity, speed, and reliability for the large-scale quantification of corresponding cyanobacterial biomass on-site, have been proven (Ha et al 2009;Srivastava et al 2013;Wang et al 2013). qPCR methods have also been used to measure geosmin-producing Streptomyces and Anabaena species, and studies have exhibiteda positive correlation between the abundance of gene copies and the corresponding geosmin concentrations (Auffret et al 2011;Su et al 2013;Tsao et al 2014). Kutovaya and Watson (2014) and Suurn kki et al (2015) developed polymerase chain reaction (PCR) protocols to detect cyanobacterial MIB/geosmin producers.…”

Section: Introductionmentioning

confidence: 99%

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Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems

et al. 2015

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2-Methylisoborneol (MIB), along with geosmin, has been frequently associated with taste and odor (T&O) events in waters, and cyanobacterial species were considered the main producers in freshwater ecosystems. The detection and quantification of genes responsible for the synthesis of these odors on-site could be valuable for the prediction and treatment of T&O. In this study, SYBR Green and TaqMan real-time quantitative polymerase chain reaction (qPCR) assays were established on the basis of conserved regions in cyanobacterial MIB cyclase gene (mic) and applied to the quantification of potential MIB-producing cyanobacteria in waters. These two qPCR assays showed the prescribed values of amplification efficiency (0.976 and 1.023, respectively) with linearity (R 2 >0.99) in the range of 10 1 to 10 7 mic copies. Examination on environmental samples and cyanobacterial cultures indicated that qPCRs were rarely inhibited by background biomass, and the limit of quantification was at the level of 10 3 mic copies L −1 in natural waters. The seasonal variations of mic abundances in Lushui Reservoir, Donghu Lake, and Qinghai Lake of China were successfully quantified using the two qPCR protocols and indicated the applicability of these assays in field monitoring. Regression analysis revealed the positive correlation between mic abundance and MIB concentration, but no significant linear correlation was found between MIB-producing cyanobacteria and total cyanobacteria in investigated waters. The qPCR assays developed in this study could rapidly and reliably detect and quantify the abundance of potential cyanobacterial MIB producers, thereby providing a valuable tool for the prediction and early detection of T&O events.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems

et al. 2015

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Growth and Depuration of Off‐Flavors in Rainbow Trout Oncorhynchus Mykiss in a Partial Recirculating Aquaculture System (PRAS)

Lindholm‐Lehto,

Kiuru

2024

Aquaculture Fish & Fisheries

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In recirculating aquaculture systems (RAS), off‐flavours accumulated in fish muscle tissue can be problematic in terms of consumer acceptance and the reputation of farmed fish products. Off‐flavours often give fish earthy, muddy, or other undesirable flavours. Typically, off‐flavours are removed during a depuration period in which fish are fasted and held in clean water. Unfortunately, this causes additional costs and delayed sales, while fish lose weight and show a decrease in lipid content. First, we studied fish growth in a partial RAS (PRAS) where the conditions are very similar to those in depuration with a water exchange rate of 4000 L kg−1 feed, compared to RAS with a 650‐L water kg−1 feed. Rainbow trout (Oncorhynchus mykiss) was reared in both systems. Our aim was to combine the benefits of a higher water exchange rate: the lack of need for biofilters and a lower accumulation of off‐flavours while obtaining stable rearing conditions. Additionally, we studied the effects of moderate feeding and H2O2 addition during depuration. The fish grew faster in a PRAS than in a RAS when fed ad libitum. Thirteen off‐flavour compounds were found in the fish flesh and 11 in the circulating water. The H2O2 addition led to decreased levels of off‐flavours in the tank water and in fish muscle. The results showed no significant differences in off‐flavours between the fed and not‐fed systems, showing that moderate feeding did not prevent a good depuration result. However, the lipid content and the overall fish weight were higher in the fed systems, which suggests more effective depuration. Increased depuration efficiency can be an important tool when considering ways to improve the profitability of production.

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Flavor Wheel for Sensory Analysis of Fish Raised in Recirculating Aquaculture Systems

Schrader

2022

N American J Aquac

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A variety of preharvest off‐flavors can occur in fish raised in recirculating aquaculture systems. Studies involving the sensory evaluations of fillets of eight different fish species that were raised in recirculating aquaculture systems either commercially or at research facilities were performed using trained panelists in two research laboratories. A flavor wheel of the types/descriptors of off‐flavors that can occur in fish that are cultured in recirculating aquaculture systems, along with general categories and degrees of objectionability, was subsequently developed from the combined data as a reference guide for producers and researchers.

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Establishment of a real-time PCR method for quantification of geosmin-producing Streptomyces spp. in recirculating aquaculture systems

Cited by 51 publications

References 36 publications

Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems

Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems

Growth and Depuration of Off‐Flavors in Rainbow Trout Oncorhynchus Mykiss in a Partial Recirculating Aquaculture System (PRAS)

Flavor Wheel for Sensory Analysis of Fish Raised in Recirculating Aquaculture Systems

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