Establishment of a safe and convenient assay for detection of HA subtype-specific antibodies with PB2 gene-knockout influenza viruses

Ito, Gakushi; Morikawa, Momoko; AKIMOTO, S.; Matsui, Tsutomu; Ozawa, Makoto

doi:10.1016/j.virusres.2021.198331

Cited by 1 publication

(1 citation statement)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, a neutralization test using the AIV subtype (Supplementary Table S1) was performed with sera from 16 blackheaded gulls. The neutralization test was performed using the method described by Ito et al (2021) [54], except that only replication-competent viruses were used for the antigen-antibody reaction. A positive result in the neutralization test was determined by confirming CPE through crystal violet staining of infected cells.…”

Section: Investigation Of Antibodies To Aivmentioning

confidence: 99%

Evaluation of the Effect of Pb Pollution on Avian Influenza Virus-Specific Antibody Production in Black-Headed Gulls (Chroicocephalus ridibundus)

Ushine

Ozawa

Nakayama

et al. 2023

Animals

Self Cite

View full text Add to dashboard Cite

Lead (Pb), an environmental pollutant, has been widely reported to have contaminated mammals, including humans and birds. This study focuses on the effects of Pb pollution on avian influenza virus (AIV) antibody production. A total of 170 black-headed gulls (Chroicocephalus ridibundus) were captured in Tokyo Bay (TBP) from January 2019 to April 2020 and in Mikawa Bay (MBP) from November 2019 to April 2021. The gulls were weighed, subjected to blood sampling, and released with a ring band on their tarsus. The samples were used to measure blood Pb levels (BLL) and AIV-specific antibodies. The BLL were compared using the Wilcoxon two-sample test between the period when black-headed gulls arrived and the wintering period, defined by the number of gulls counted in each area. A significant increase was found in the TBP. A decrease in BLL significantly increased antibody titer during wintering in TBP and MBP. Pb pollution had a negative effect on the production of AIV antibodies. These findings suggest that wild birds that were contaminated by Pb in the environment may facilitate the spread of zoonotic diseases, further increasing the possibility that environmental pollutants may threaten human health.

show abstract

Section: Investigation Of Antibodies To Aivmentioning

confidence: 99%