Establishment of a Secondary Infection Model of Severe Acute Pancreatitis in Swine

Wang, Jianhua; Shao, Chengwei; Zuo, Changjing; Zheng, Jianming; Hao, Jing; Shao, Chenghao; Zhang, Feng; Sun, Gaofeng; Liu, Yan

doi:10.1097/mpa.0b013e3181f7e2ec

Cited by 1 publication

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References 31 publications

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“…Among these bile salts, the taurine‐conjugated bile salt Na‐TC is the most widely used and best characterized to date in inducing AP. The Na‐TC ductal infusion model has been shown to induce pancreatic oedema, haemorrhage and necrosis in large animals, such as rabbits, 62 swans, 63 dogs 64 and pigs 65,66 . However, the pancreatic injury in these large animals is not sufficient to produce multiple organ dysfunction syndromes (MODS).…”

Section: Induction Of Ap By Bile Saltsmentioning

confidence: 99%

Review of experimental animal models of biliary acute pancreatitis and recent advances in basic research

Wan

Huang

Latawiec

et al. 2012

HPB

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Acute pancreatitis (AP) is a formidable disease, which, in severe forms, causes significant mortality. Biliary AP, or gallstone obstruction-associated AP, accounts for 30-50% of all clinical cases of AP. In biliary AP, pancreatic acinar cell (PAC) death (the initiating event in the disease) is believed to occur as acinar cells make contact with bile salts when bile refluxes into the pancreatic duct. Recent advances have unveiled an important receptor responsible for the major function of bile acids on acinar cells, namely, the cell surface G-protein-coupled bile acid receptor-1 (Gpbar1), located in the apical pole of the PAC. High concentrations of bile acids induce cytosolic Ca(2+) overload and inhibit mitochondrial adenosine triphosphate (ATP) production, resulting in cell injury to both PACs and pancreatic ductal epithelial cells. Various bile salts are employed to induce experimental AP, most commonly sodium taurocholate. Recent characterization of taurolithocholic acid 3-sulphate on PACs has led researchers to focus on this bile salt because of its potency in causing acinar cell injury at relatively low, sub-detergent concentrations, which strongly implicates action via the receptor Gpbar1. Improved surgical techniques have enabled the infusion of bile salts into the pancreatic duct to induce experimental biliary AP in mice, which allows the use of these transgenic animals as powerful tools. This review summarizes recent findings using transgenic mice in experimental biliary AP.

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Section: Induction Of Ap By Bile Saltsmentioning

confidence: 99%

Review of experimental animal models of biliary acute pancreatitis and recent advances in basic research

Wan

Huang

Latawiec

et al. 2012

HPB

View full text Add to dashboard Cite

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Establishment of a Secondary Infection Model of Severe Acute Pancreatitis in Swine

Abstract: The swine model of SAP secondary infection was successfully developed using a 2-step method, which could serve as a platform for SAP studies that need complex experimental manipulations for longer time spans, especially for imaging research.

Cited by 1 publication

References 31 publications

Review of experimental animal models of biliary acute pancreatitis and recent advances in basic research

Review of experimental animal models of biliary acute pancreatitis and recent advances in basic research

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