Establishment of a set of wheat-rye addition lines with resistance to stem rust

Liu, Chang; Wang, Jing; Fu, Shulan; Wang, Long; Li, Hongwei; Wang, Mian; Huang, Yuhong; Shi, Qinghua; Zhou, Yonghong; Guo, Xianrui; Zhu, Congle; Zhang, Jing; Han, Fangpu

doi:10.1007/s00122-022-04127-7

Cited by 13 publications

(12 citation statements)

References 60 publications

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“…The wheat cultivar parent 843, the diploid rye parent PI 613196, and the set of wheat-rye disomic and telosomic addition lines developed by distant hybridization between 843 and PI 613196 were kindly provided by Pro. Fangpu Han, institute of genetics and developmental biology, Chinese Academy of Sciences, Beijing (Liu et al 2022). Wheat cultivars Gao 8901, Shixin 828 and Shiluan 02 − 1 sustainable to powdery mildew were used as recurrent parents and maintained in our lab.…”

Section: Plant Materialsmentioning

confidence: 99%

Development of novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance and physical mapping of the resistance gene PmW6RS

Wang

Han

Liu

et al. 2023

Preprint

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Rye (Secale cereale L., RR) contains valuable genes for wheat improvement. However, most of the rye resistance genes have not been successfully used in wheat cultivars. Identification of new rye resistance genes and transfer these genes to wheat by developing small fragment translocation lines will make these genes more usable for wheat breeding. In this study, a broad-spectrum powdery mildew resistance gene PmW6RS was located on rye chromosome arm 6RS using a new set of wheat-rye disomic and telosomic addition lines. To further study and use PmW6RS, 164 wheat-rye 6RS translocation lines were developed by 60Coγ ray irradiation. Seedling and adult stage powdery mildew resistance analysis showed that 106 of the translocation lines were resistant. Physical map of 6RS was constructed using the 6RS translocation and deletion lines, and PmW6RS was located in the 6RS-0.58-0.66-bin, flanked by markers X6RS-3 and X6RS-10 corresponding to the physical interval of 50.23-68.61 Mb, and was 18.38 Mb in Weining rye genome. A total of 23 resistance-related genes were annotated. Nine markers co-segregate with the 6RS-0.58-0.66-bin, which can be used to rapidly trace the 6RS fragment carrying PmW6RS. Small fragment translocation lines with powdery mildew resistance were backcrossed with wheat major cultivars and 39 homozygous 6RS small fragment translocation lines were obtained. In conclusion, this study not only provide novel gene source and germplasms for wheat resistance breeding, but also laid solid foundation for further cloning of PmW6RS.

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Section: Plant Materialsmentioning

confidence: 99%

Development of novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance and physical mapping of the resistance gene PmW6RS

Wang

Han

Liu

et al. 2023

Preprint

Self Cite

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“…These three Pst races were used in stripe rust resistance tests in the seedling stage. CYR32 is virulent toward Yr1, 2, 3,4,6,7,8,9,11,12,13,14,16,17,18,25,27,28,29,30,31,32,43,44, A, Alba, Cle, CV, Gaby, Res, SD, SO, Exp2, Sk, and SpP. CYR33 is virulent toward Yr1, 2, 3,4,6,7,8,9,11,12,17,18,25,28,29,30,31,32, A, and Su.…”

Section: Resistance Analysismentioning

confidence: 99%

“…CYR32 is virulent toward Yr1, 2, 3,4,6,7,8,9,11,12,13,14,16,17,18,25,27,28,29,30,31,32,43,44, A, Alba, Cle, CV, Gaby, Res, SD, SO, Exp2, Sk, and SpP. CYR33 is virulent toward Yr1, 2, 3,4,6,7,8,9,11,12,17,18,25,28,29,30,31,32, A, and Su. CYR34 is virulent toward Yr1, 2, 3,4,6,7,8,9,10,11,12,13,14,16,17,…”

Section: Resistance Analysismentioning

confidence: 99%

“…CYR33 is virulent toward Yr1, 2, 3,4,6,7,8,9,11,12,17,18,25,28,29,30,31,32, A, and Su. CYR34 is virulent toward Yr1, 2, 3,4,6,7,8,9,10,11,12,13,14,16,17,18,19,24,25,26,27,28,29,30,31,32,43,44, Exp2, Sp, A, and Sk [26]. All Pst races were provided by the Plant Protection Institute, Gansu Academy of Agricultural Sciences, China.…”

Section: Resistance Analysismentioning

confidence: 99%

“…Therefore, many researchers are focusing their attention on chromosomes 2R to 7R of rye. For example, Liu et al [29] reported that a 3RL addition line exhibited high resistance to stem rust (Ug99). Ren et al [30] reported that a 7RL.7BS translocation line exhibited resistance to stripe rust, powdery mildew, and Fusarium head blight.…”

Section: Introductionmentioning

confidence: 99%

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Development and Molecular Cytogenetic Characterization of a Novel Wheat-Rye T6RS.6AL Translocation Line from Secale cereale L. Qinling with Resistance to Stripe Rust and Powdery Mildew

Ren

Sun

Ren

et al. 2022

IJMS

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In this study, a novel T6RS.6AL translocation line, 117-6, was selected from a cross between common Chuannong25 (CN25) wheat and Qinling rye. The results of nondenaturing fluorescence in situ hybridization (ND-FISH) and PCR showed that 117-6 contained two T6RS.6AL translocation chromosomes. The distal region of the 6RS chromosome in 117-6 was mutant and showed different FISH signal patterns. When inoculated with different stripe rust races and powdery mildew races in seedlings, 117-6 expressed high resistance to them. The 117-6 line also exhibited high resistance to stripe rust and powdery mildew in the field under natural Puccinia striiformis f. sp. tritici (Pst) and Blumeria graminis f. sp. tritici (Bgt) infection. The cytogenetic analysis indicated that the introduction of 6RS conferred resistance ability. Compared with wheat parent CN25, 117-6 exhibited excellent agronomic traits in the field. The present study indicated that Qinling rye may carry favorite genes as a potential source for wheat genetic improvement, and 117-6 could be a useful germplasm for wheat breeding programs in the future.

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Development of novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance and physical mapping of the resistance gene PmW6RS

Wang,

Han,

Liu

et al. 2023

Theor Appl Genet

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Establishment of a set of wheat-rye addition lines with resistance to stem rust

Cited by 13 publications

References 60 publications

Development of novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance and physical mapping of the resistance gene PmW6RS

Development of novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance and physical mapping of the resistance gene PmW6RS

Development and Molecular Cytogenetic Characterization of a Novel Wheat-Rye T6RS.6AL Translocation Line from Secale cereale L. Qinling with Resistance to Stripe Rust and Powdery Mildew

Development of novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance and physical mapping of the resistance gene PmW6RS

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