Establishment of a stable urethral stricture model in New Zealand rabbits

Zheng, Jie; Ding, Qiang; Sun, Ch eng-shu; Li, B.; Sun, Yufang; Zhao, Xinshu; Ch, Feng; Fang, Zhaoxi

doi:10.1016/j.acuroe.2012.04.016

Cited by 3 publications

(5 citation statements)

References 16 publications

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“…results(28). In contrast to the previous methods, for the method proposed in the present study, the instruments required are available at most hospitals, and the bleeding volume is less than that with open surgery.…”

mentioning

confidence: 75%

Three new experimental models of anterior urethral stricture in rabbits

Yao¹,

Wei²,

Wan³

et al. 2022

Transl Androl Urol

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Background: This study describes and compares three surgical procedures for the construction of urethral stricture (US) models in rabbits.Methods: Forty adult male rabbits were allocated to four groups: 36 rabbits were randomly assigned to three experimental groups, while the remaining 4 were assigned to a sham group. The penis was separated from the rectum. Then along the ventral midline, a longitudinal penile skin incision was made while ensuring that the urethral mucosa was intact and the muscular layer was not completely incised. In group 1 (n=12), ventral semi-circumferential mucosa electrocoagulation of a 1-cm length of the anterior urethra was performed until ulceration occurred. In group 2 (n=12), the ventral urethral mucosa was incised, and electrocoagulation of the dorsal semi-circumferential mucosa was performed. In group 3 (n=12), wholecircumferential mucosa electrocoagulation was performed. In group 4 (n=4), no special treatment was performed. Four weeks later, urethrography, urethroscopy, and histological evaluation were carried out. Results:The weights of the rabbits in the four groups were comparable. There was no significant difference between groups 2 and 3 with regard to operative time, but the operative time in these groups was significantly longer than that in group 1 (group 2 vs. group 1: P<0.05, group 3 vs. group 1: P<0.001).After the surgery, urinary fistula with infection occurred in one rabbit in group 1, and one rabbit died due to urethral atresia in group 3. According to the urethrography and urethroscopy findings, 9 out of 12 rabbits in group 1, 5 out of 12 rabbits in group 2, and 11 out of 11 rabbits in group 3 developed US, while no rabbits in the sham group developed US. Histopathological examination revealed injury to the urothelium, inflammatory infiltration, a decrease in the amount of blood vessels and smooth muscle fibers, and a decrease in the amount of collagen fibers.Conclusions: Compared with the semi-circumferential procedures, the whole-circumferential procedure had a higher success rate. Therefore, this procedure seems to have potential for the construction of longsegment rabbit US models.

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mentioning

confidence: 75%

Three new experimental models of anterior urethral stricture in rabbits

Yao¹,

Wei²,

Wan³

et al. 2022

Transl Androl Urol

View full text Add to dashboard Cite

show abstract

“…Differences in the results obtained and described in the literature are caused by different research subjects: horse urethra, etc. [32,37,39]. In case of the tests of Natali et al [13,39], the results of pressuredeformation dependence were given only for a range of elastic deformations not exceeding 0.6 mm/mm.…”

Section: Resultsmentioning

confidence: 99%

“…The maximum urinary pressure was 4kPa [26,32]. In the literature, this value appears many times, hence, it is possible to compare the results obtained by other authors in the experimental and model studies [23,32,[37][38][39]. In the scope of the CFD flow dynamics analysis, the methodology of calculations, appropriate boundary and beginning conditions as well as the structure and fluid properties were proposed.…”

Section: Cijmentioning

confidence: 99%

Numerical Analysis of Deformation and Flow in the Proximal Area of the Urethra

Rudyk

Malinowski

Mackiewicz

et al. 2020

International Journal of Applied Mechanics and Engineering

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Pathological conditions of a male urethra, including fibrosis, have a mechanical background along the entire length of the urethra. They may be caused by excessive deformation of the urethra locally or globally. The condition of prolonged overload causes abnormal tissue remodelling and, consequently, the formation of a thick layer of scar tissue differentiated from the connective tissue of the urethra. This tissue, which has higher mechanical properties, is not highly deformable and therefore, causes a decrease in the diameter of the urethra, which results in conditions that disturb the natural flow of urine. In this paper, it was decided to determine the deformation conditions in the proximal part of the urethra. The study was conducted in three main stages. Transverse sections of the animal urethral tissues were prepared in order to examine mechanical properties and perform histological examinations. On the basis of these examinations, material models which fitted best for the experimental results were sought. Material constants of the Mooney-Rivlin material model with the best fit ratio were determined for further research. On the basis of histological photographs, a geometrical and numerical model of the urethra was developed. The urethra was tested in a flat state of deformation. The strain and stress fields of the Caucha tensor were examined. The methodology of testing the dynamics of the urine flow in the highly deformable urethra was proposed. This is important for the analysis of the influence of at excessive pressure on pathological tissue remodelling leading to fibrosis.

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“…On the other hand, pediatric endoscopic instruments, which are expensive, difficult to handle precisely and only available in institutions with pediatric urology departments, are not needed. Additionally, these procedures do not require a microscope or even optical magnification, as described in studies of urethral tissue resection (10). Finally, blood loss should be significantly less with electrocoagulation rather than resection of a urethral segment.…”

Section: Editorialmentioning

confidence: 99%

“…Such failures relate most likely to the mechanism of injury, which should be severe; otherwise, the abundant blood supply of the corpus spongiosum may lead to proper healing preventing hyperplastic scar formation. Both endoscopic electroresection of urethral tissues (13,14) and open excision of urethral segments (10,15) have demonstrated high SR approaching 100%. These surgical techniques construct a model of urethral defect, but not a genuine US, possibly not reflect the actual process of the human US, also lacking some typical histologic features of it, such as the presence of urothelium.…”

Section: Editorialmentioning

confidence: 99%

In search of the ideal animal model for urethral strictures for therapeutic purposes

Karapanos¹

2022

Transl Androl Urol

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Establishment of a stable urethral stricture model in New Zealand rabbits

Cited by 3 publications

References 16 publications

Three new experimental models of anterior urethral stricture in rabbits

Three new experimental models of anterior urethral stricture in rabbits

Numerical Analysis of Deformation and Flow in the Proximal Area of the Urethra

In search of the ideal animal model for urethral strictures for therapeutic purposes

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