Establishment of a universal and rational gene detection strategy through three-way junction-based remote transduction

Tang, Yidan; Lu, Baiyang; Zhu, Zhentong; Li, Bingling

doi:10.1039/c7sc03190d

Cited by 57 publications

(38 citation statements)

References 47 publications

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“…Notably, here the similar kinetic curves generated for different concentrations of ompA genes indicates 1.5 h LAMP reaction could be enough to consume up all the primers, no matter how few the template is. This is consistent with previous studies [21,31]. While, when we execute the LAMP with the ompA embedded in 10% fecal samples (Fig.…”

Section: Lamp-3w-cha-pgm Detection Of Mimic and Synthetic Gene Targetssupporting

confidence: 92%

“…Through this process domain a will be close enough to the domain 2-3 in the TP, finally forming an associative trigger to start downstream CHA circuit (thus also called 3W-CHA). The 3W-CHA circuit pathway should be similar to any classic one [31]. The associative trigger opens H1 and then H2 through two successive OSD reactions, forming hybrid H1:H2 as the final product.…”

Section: Principle Of Lamp-3w-cha-pgm Detectionmentioning

confidence: 99%

“…The ompA gene has been selected as our model target. Its five primer set for LAMP has been selfdesigned and proven very efficient in our previous publications [31]. They can amplify down to 20 copies of selected 236-mer gene segment within 1.5 h (Fig.…”

Section: Lamp-3w-cha-pgm Detection Of Mimic and Synthetic Gene Targetsmentioning

confidence: 99%

“…1, the basic principle says that only the domain b in TP, and domain a in CHA H1 were relevant to the T1. Therefore, except domain b* of TP, and a* of H1, we directly borrow all other CHA component sequences from a well-performed CHA circuit previously designed for other purpose [31,35]. Classic fluorescence resonance energy transfer (FRET) experiments have been firstly performed to test the efficiency of 3W-CHA reaction (scheme shown in Fig.…”

Section: Lamp-3w-cha-pgm Detection Of Mimic and Synthetic Gene Targetsmentioning

confidence: 99%

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One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers

Guo

Dong

et al. 2020

Analytica Chimica Acta

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Section: Lamp-3w-cha-pgm Detection Of Mimic and Synthetic Gene Targetssupporting

confidence: 92%

Section: Principle Of Lamp-3w-cha-pgm Detectionmentioning

confidence: 99%

Section: Lamp-3w-cha-pgm Detection Of Mimic and Synthetic Gene Targetsmentioning

confidence: 99%

Section: Lamp-3w-cha-pgm Detection Of Mimic and Synthetic Gene Targetsmentioning

confidence: 99%

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One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers

Guo

Dong

et al. 2020

Analytica Chimica Acta

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“…The success of this strand displacement reaction depends on the formation of a stable four‐way junction bridged by TH:TH* and BM:BM* hybridization. This process is thus called four‐way junction‐based transduction, shortened to 4W‐transduction . Correspondingly, the TP1:TP2 complex is called a 4W‐associative trigger.…”

Section: Methodsmentioning

confidence: 99%

Homogeneous and Universal Detection of Various Targets with a Dual‐Step Transduced Toehold Switch Sensor

Tang

2020

ChemBioChem

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Figure 3. Detectionoft hrombin. A) Schematic representationo fS Dt ransduction (Step I) for thrombin detection.B inding of the thrombin to the Blocker to release TP1. B) 12 %native PAGE analysis of hybridization between Blocker and TP1 with and withoutt hrombin. Lane Mt olane 3: ladder,TP1, Blocker and TP1,Blocker,TP1, and thrombin. C) Specificityc haracterization with ab ar graph representing endpoint detection at 2hcolor reaction.

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Biocomputing for Portable, Resettable, and Quantitative Point‐of‐Care Diagnostics: Making the Glucose Meter a Logic‐Gate Responsive Device for Measuring Many Clinically Relevant Targets

Zhang

2018

Angewandte Chemie

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It is recognized that biocomputing can provide intelligent solutions to complex biosensing projects.H owever, it remains challenging to transform biomolecular logic gates into convenient, portable,r esettable and quantitative sensing systems for point-of-care (POC) diagnostics in alow-resource setting.T oo vercome these limitations,t he first design of biocomputing on personal glucose meters (PGMs) is reported, which utilizes glucose and the reduced form of nicotinamide adenine dinucleotide as signal outputs,DNAzymes and protein enzymes as building blocks,a nd demonstrates ag eneral platform for installing logic-gate responses (YES,N OT, INHIBIT,N OR, NAND,a nd OR) to av ariety of biological species,s uch as cations (Na + ), anions (citrate), organic metabolites (adenosine diphosphate and adenosine triphosphate) and enzymes (pyruvate kinase,a lkaline phosphatase, and alcohol dehydrogenases). Ac oncatenated logical gate platform that is resettable is also demonstrated. The system is highly modular and can be generally applied to POC diagnostics of many diseases,s uch as hyponatremia, hypernatremia, and hemolytic anemia. In addition to broadening the clinical applications of the PGM, the method reported opens anew avenue in biomolecular logic gates for the development of intelligent POC devices for on-site applications. Figure 4. "Concatenated" logic gate using both NADH and glucose as the output. a) Scheme of the biocatalyticcascade with multiple inputs and outputs. b) The PGM response of repeated switches between "off" and "on" states by adding appropriate inputs of enzyme substrates and coenzymes.

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Establishment of a universal and rational gene detection strategy through three-way junction-based remote transduction

Abstract: The polymerase chain reaction and many isothermal amplifications are able to achieve super gene amplification.

Cited by 57 publications

References 47 publications

One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers

One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers

Homogeneous and Universal Detection of Various Targets with a Dual‐Step Transduced Toehold Switch Sensor

Biocomputing for Portable, Resettable, and Quantitative Point‐of‐Care Diagnostics: Making the Glucose Meter a Logic‐Gate Responsive Device for Measuring Many Clinically Relevant Targets

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