Establishment of Adult Rat Schwann Cell Cultures: Effect of b-FGF, α-MSH, NGF, PDGF, and TGF-β on Cell Cycle

A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves

“…4a, revealed that cells rapidly expanded in number and remained highly viable throughout a time period of 12 days after culture initiation. Data analysis of the growth curves determined that the cells duplicated in number every 2.2 days on average, which was consistent with previous reports61925. Importantly, this data showed that total cell yields in the order of 8 × 10 6 cells per nerve biopsy can be obtained as soon as 10 days after the initial plating.…”

Effect of bFGF on the MCF-7 Cell Cycle with CD44+/CD24-: Promoting the G0/G1→G2/S Transition

“…Today, FGF proteins are understood to comprise a growth factor family that includes more than 20 proteins [9]. bFGF is mutagenic in various cell types, including bovine aortic endothelial cells [10] and adult rat schwann cells [11]; bFGF produces a 4-fold increase in DNA synthesis and a 3-fold rise in BrdU labeling, suggesting that it promotes the G1/S transition [12]. When added to culture medium, the proportion of nifedipine-reactive patient cells that underwent progression to the S and G2/M phases from the G0/G1 phase increased significantly compared to the proportion in nifedipine-non-reactive patient cells [13]; bFGF plays a concentration-dependent role regulating the cell cycle [12,14,15].…”

Differences of Cell Growth and Cell Cycle Regulators Induced by Basic Fibroblast Growth Factor Between Nifedipine Responders and Non-responders

“…Basic-FGF is known to be mitogenic for various cell types, including bovine aortic endothelial cells (23), Chinese hamster lung fibroblasts (24), and adult rat schwann cells (25). When added into the culture medium, bFGF induces DNA synthesis in human gingival epithelial cells (26) and is able to sustain cell proliferation of oligodendrocyte type-2 astrocyte progenitor cells (27).…”