Establishment of adventitious root culture of Stevia rebaudiana Bertoni in a roller bottle system

Reis, Rafael Valim; Borges, Ana Paula Patrão Luis; Chierrito, Talita Perez Cantuaria; Souto, Eliezer Rodrigues de; Souza, Lauro Mera de; Iacomini, Marcello; Oliveira, Arildo José Braz de; Gonçalves, Regina Aparecida Correia

doi:10.1007/s11240-011-9925-7

Cited by 57 publications

(29 citation statements)

References 18 publications

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“…Regarding the plant organ cultures, root culture is more favorable than the shoot culture because of rapid root multiplication and the ability of cultivation in large-scale bioreactors. The adventitious root cultures induced by plant growth regulators (PGRs) have been studied in various medicinal plants species such as Valeriana amurensis (Cui et al 2011), Stevia rebaudiana (Reis et al 2011), and Morinda citrifolia (Baque et al 2010). In addition, adventitious root cultures of some medicinal plant species, e.g.…”

Section: Introductionmentioning

confidence: 99%

Induction, characterization, and NMR-based metabolic profiling of adventitious root cultures from leaf explants of Gynura procumbens

Saiman

Mustafa

Schulte³

et al. 2012

Plant Cell Tiss Organ Cult

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Gynura procumbens is a medicinal plant used in South East Asia to treat various ailments such as rash, hemorrhoids, inflammation, and diabetes. In order to develop a large-scale culture system for G. procumbens biomass containing bioactive compounds, adventitious root cultures were initiated from leaf explants. Murashige and Skoog (MS) media containing different compositions of indole-3-butyric acid (IBA), 1-naphthalene-acetic acid (NAA), and combinations of both plant growth regulators (PGRs) were evaluated for root induction. A combination of 3 mg/l NAA ? 1 mg/l IBA gave the highest root induction (48%) as compared to other PGRs treatments after 9 weeks of incubation period. Subsequently, the adventitious roots were established in liquid culture containing MS medium and the combination of 3 mg/l NAA ? 1 mg/l IBA. A study on the medium strength, sucrose concentration, pH, and light versus dark was conducted to optimize the in vitro culture conditions. The results showed that differences in MS medium strength from half to double strength, and light or dark condition did not significantly affect the biomass production, while the initial medium pH of 5.5 and 2% w/v sucrose concentration were most suitable for the root culture growth. Nuclear magnetic resonance (NMR) spectroscopy was performed to characterize the metabolite content in the root cultures of G. procumbens. Among the elucidated metabolites were some phenylpropanoids identified as caffeic acid, chlorogenic acid, and 3,5-di-O-caffeoylquinic acid which might be the bioactive compounds associated to the folk use of this plant.

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Section: Introductionmentioning

confidence: 99%

Induction, characterization, and NMR-based metabolic profiling of adventitious root cultures from leaf explants of Gynura procumbens

Saiman

Mustafa

Schulte³

et al. 2012

Plant Cell Tiss Organ Cult

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“…This is in accordance with our study. Individual treatment of NAA promoted adventitious root proliferation in Stevia rebaudiana (Reis et al, 2011) and in Aloe vera (Lee et al, 2011). Even though in adventitious root culture of Aloe vera , NAA(0.5 mgl -1 ) enhanced root proliferation there was no significant increase in biomass and dry weight increased only when IBA get combined with NAA.…”

Section: Adventitious Root Induction and Proliferation From In Vitro mentioning

confidence: 99%

“…For adventitious root induction, various plant materials like in vitro root, leaf and in vivo leaf, root as explants have been studied in the following plants: In vitro roots; Astragalus membranaceus ; Stevia rebaundiana (Reis et al, 2011) and Eleutherococcus koreanum (Lee and Pack et al, 2012). In vitro leaf explants in; Chicorium intybus (Nandagopal and kumari, 2007); Orthosiphon stamineus ( Ling et al, 2009); Plumbago zeylanica (Sivanesan and Jeong, 2009); Periploca sepium (Zhang et al, 2011) and Withania somnifera (Sivanandhan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Root Culture From Roots And Leaf Explants Of Luffa Acutangula(L.) Roxb

Umamaheswari¹,

Ambethkar²,

Margaret³

et al. 2014

IJIRSET

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An efficient protocol had been developed for the root culture of Luffa acutangula (L.) Roxb. The root and leaf explants from 21 day old in vitro raised seedlings were cultured on half strength Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of Indole-3-acetic acid(IAA), Indole-3-butyric acid (IBA) and Napthalene acetic acid (NAA). IBA at 1.0 mgl -1 combined with NAA (1.0 mgl -1 ) induced maximum percentage of rooting from leaf explants, under total dark condition. After 3 weeks, well established roots were separated. Fresh root tissue from the leaf explants and root tips from in vitro seedlings were subcultured in half strength MS liquid medium supplemented with different concentrations and combinations of IAA, IBA and NAA, under continuous agitation at 80 rpm under total dark condition. The biomass of root culture (derived from leaf explants) in the MS medium fortified with IBA(1.0 mgl -1 ), NAA(1.0mgl -1 ) and L-glutamine (20 mgl -1 ) was increased to 5.38 g FW and the biomass of root culture (derived from root tip) in the MS medium supplemented with IBA (0.5 mgl -1 ), NAA (1.0 mgl -1 ) and L-glutamine (20 mgl -1 ) was increased to 5.82 g FW after 7 weeks of culture.

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“…The advantages of adventitious root culture are the root not influenced by geotropism, growth of root branch fast and had a genetic stability. Many researches have been conducted to increase biomass and secondary metabolite by adventitious roots in bioreactor, for examples, producing whitanolide-A in adventitious root of Whitania somnifera (Praveen and Murthy, 2010;Sivanandhan et al, 2012), increasing biomass and secondary metabolite of Glycyrrhiza uralensis Fisch (Yin et al, 2014), increasing bioreactor capacity to produce biomass and cafeic acid derivatives in adventitious root culture of Echinacea angustifolia (Cui et al, 2013), production of adventitious root biomass of Podophyllum hexandrum Royle (Rajesh et al, 2014), Astragalus membranaceus (Wu et al, 2011), Stevia reboudiana Bertoni (Reis et al, 2011) and Panax ginseng (Wang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Culture Conditions of Talinum paniculatum Gaertn. Adventitious Roots in Balloon Type Bubble Bioreactor Using Aeration Rate and Initial Inoculum Density

Manuhara¹,

Kristanti²,

Utami³

2015

Asian J. of Biological Sciences

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Optimization of culture conditions of Talinum paniculatum Gaertn. adventitious roots in the balloon type bubble bioreactor have been done in order to increase its production of adventitious roots and saponin content. Culture conditions were used in this research were combination of aeration rate (0.25, 0.5 and 0.75 vvm) and initial inoculum density (0.5, 1, 2 g/400 mL). Bioreactor with a volume of 1000 mL was filled with 400 mL of liquid MS medium supplemented with IBA 2 mg LG 1 were then given sterile air through a microfilter (0.2 µm) with different air flow rates. Into each bioreactor were added of different inoculum density of adventitious roots that had previously been induced from leaf explants of T. paniculatum on solid MS medium supplemented with IBA 2 mg LG 1 . Cultures were maintained for 14 days and sampling was done for every two days to determine the sugar content, conductivity and pH of the medium. The results showed that the combination of aeration rate of 0.5 vvm and inoculum density of 1 g/400 mL was the best treatment that can increase biomass of adventitious roots, whereas the combination of aeration rate of 0.75 vvm and inoculum density of 2 g/400 mL was the best treatment that can be increased of saponin content.

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Establishment of adventitious root culture of Stevia rebaudiana Bertoni in a roller bottle system

Cited by 57 publications

References 18 publications

Induction, characterization, and NMR-based metabolic profiling of adventitious root cultures from leaf explants of Gynura procumbens

Induction, characterization, and NMR-based metabolic profiling of adventitious root cultures from leaf explants of Gynura procumbens

In Vitro Root Culture From Roots And Leaf Explants Of Luffa Acutangula(L.) Roxb

Optimization of Culture Conditions of Talinum paniculatum Gaertn. Adventitious Roots in Balloon Type Bubble Bioreactor Using Aeration Rate and Initial Inoculum Density

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