Establishment of adventitious root culture system of Cynanchum wilfordii in air-lift bioreactors for the efficient production of bioactive compounds

GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays

“…The extraction of shoots with forceps from in vitro culture containers, as well as from TIS bioreactors, followed by arranging them on sterile surfaces in order to divide them with the help of surgical scalpels and their subsequent introduction into new culture containers, a process known as subculturing in the conventional in vitro culture process, is time-consuming and represents a significant portion the hand labor involved in most in vitro plant culture processes. The components of the actual GreenTray ® TIS bioreactor described herein, which is a further evolution of the ones presented in the past [31,32], make it significantly improved and versatile compared to other commercial TIS bioreactors, such as RITA ® , Matis ® , Plantform TM , SETIS TM [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17], or the TIS developed by several in vitro plant culture laboratories oriented to solve particular propagation objectives for diverse plant species [18][19][20][21][22][23][24][25][26][27][28].…”

“…The best-known commercial ones are RITA ® , its bigger version Matis ® [1][2][3][4][5][6][7], Plantform TM [8][9][10][11][12][13][14], and SETIS TM [15][16][17]. In addition, there are several other TISs developed for particular purposes by in vitro plant culture laboratories [18][19][20][21][22][23][24][25][26][27][28]. With some differential characteristics among them, there are two common properties which make them similar and help to differentiate the GreenTray ® .…”

GreenTray^®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays

“…Extraction of shoots with forceps form the in vitro culture containers, as well as from TIS bioreactors, arranging them on sterile surfaces in order to divide them with the help of surgical scalpels, followed by their introduction into new culture containers, process known as performing a subculture in the conventional in vitro culture process, is time consuming and represents great part of the hand labor expense dedicated to the perform most of the plant in vitro culture processes. The components of the actual GreenTray ® TIS bioreactor described herein, which is a further evolution of the ones presented in the past [32,33], make it significantly improved and versatile compared to other commercial TIS bioreactors, such as RITA ® , Matis ® , Plantform TM , SETIS TM [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20], or the TIS developed by several plant invitro culture laboratories oriented to solve particular propagation objectives for diverse plant species [21][22][23][24][25][26][27][28][29][30][31].…”

“…The best-known commercial ones are RITA ® , its bigger version Matis ® [4][5][6][7][8][9][10], Plantform TM [11][12][13][14][15][16][17], and SETIS TM [18][19][20]. Besides, there are several other TIS developed for their particular purposes by plant invitro culture laboratories [21][22][23][24][25][26][27][28][29][30][31]. With some differential characteristics among them, there are two common properties, which make them similar, and help to differentiate the GreenTray ® .…”