Establishment of adventitious root cultures from leaf explants of Plumbago rosea and enhanced plumbagin production through elicitation

Silja, P. K.; Satheeshkumar, K.

doi:10.1016/j.indcrop.2015.07.021

Cited by 35 publications

(10 citation statements)

References 49 publications

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“…Chitosan was reported to increase phytoalexin production in R. graveolens [41], stimulate lignans accumulation in Schisandra chinensis [19] and affect higher accumulation of TPC in Orthosiphon stamineus [42]. Moreover, the application of chitosan, yeasts extract or precursor feeding in elicitation of PLU in Plumbago roots cultures is popular [11,12,43,44]. Comparing these works to our results, elicited D. muscipula plants accumulate more PLU and other phenolic derivatives.…”

Section: Impact Of Biotic Elicitation On Plant Growth and Secondary Msupporting

confidence: 75%

Elicitation-Based Method for Increasing the Production of Antioxidant and Bactericidal Phenolic Compounds in Dionaea muscipula J. Ellis Tissue

Makowski

Tokarz

et al. 2020

Molecules

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The carnivorous plant Dionaea muscipula J. Ellis (Venus flytrap) is a widely known medical herb, capable of producing various phenolic compounds known for their strong antioxidant and antibacterial properties. In the pharmaceutical industry, Venus flytrap is grown in tissue cultures, as the natural population of D. muscipula is very limited. Here, we describe an improved method to increase the quantity and quality of phenolic compounds produced in D. muscipula. This is achieved by combining biotic elicitation (using Cronobacter sakazakii bacteria lysate) of D. muscipula cultured with rotary shaking (hydromechanical stress), which we describe here for the first time. The antibacterial activity and the antioxidant properties of the obtained compounds were studied on two antibiotic-resistant human pathogenic bacteria. The proposed plant culture conditions resulted in an increase in fresh weight, as well as a higher total phenolic content, in comparison to traditional tissue cultures on agar-solidified medium. With the use of high-performance liquid chromatography, we demonstrated that the described elicitation strategy leads to an increased synthesis of myricetin, caffeic acid, ellagic acid and plumbagin in D. muscipula tissue. We also found that a higher level of antioxidant activity, exhibited by the plant extract, corresponded with its higher phenylpropanoid content. The bactericidal activity of the extract against Staphylococcus aureus was dependent on the duration of plant culture under described elicitation conditions, whereas neither elicitation condition (duration or elicitor concentration) seemed relevant for the bactericidal activity of the extract towards Escherichia coli. This suggest that Gram-negative bacteria are less sensitive to compounds derived from Venus flytrap tissue.

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Section: Impact Of Biotic Elicitation On Plant Growth and Secondary Msupporting

confidence: 75%

Elicitation-Based Method for Increasing the Production of Antioxidant and Bactericidal Phenolic Compounds in Dionaea muscipula J. Ellis Tissue

Makowski

Tokarz

et al. 2020

Molecules

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“…Fungal components can be used as elicitor to induce productions of various types of secondary metabolites (Gao, Yong, & Dai, ). In order to enhance the production of compounds, yeast has become an effective elicitor (Mahunu et al, ; Silja & Satheeshkumar, ); Zhao & Sakai, . In present study, P. pastoris , which is also one kind of yeast, could pose a significant enhancement on the accumulation of metabolites.…”

Section: Discussionmentioning

confidence: 64%

“…In order to enhance the production of compounds, yeast has become an effective elicitor (Mahunu et al, 2016;Silja & Satheeshkumar, 2015); Zhao & Sakai, 2003. In present study, P. pastoris, which is also one kind of yeast, could pose a significant enhancement on the accumulation of metabolites.…”

Section: Discussionmentioning

confidence: 90%

“…In Populus davidiana × P. alba var. pyramidalis seedlings, the P. pastoris recombinant protein rEpl1-p induction altered the expression levels of genes in the salicylic acid, jasmonic acid, and auxin signal transduction pathways (Yu et al, 2018).Among these, yeasts appeared to be a kind of effective elicitors to activate the secondary compound content in plant (Silja & Satheeshkumar, 2015;Zhao & Sakai, 2003). In previous studies, the overexpression of Ydj1p, Ssa1p and Sec63p in recombinant P. pastoris increased the expression level of CalB by 1.6-, 1.4-, and 1.4-fold, respectively, compared to the control strain containing only the CalB gene (Samuel, Vadhana, Kamatchi, Antony, & Meenakshisundaram, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Induction of signal molecules and expression of functional genes after Pichia pastoris stimulation in Glycyrrhiza uralensis Fisch adventitious roots

et al. 2019

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Glycyrrhiza uralensis Fisch is threatened by over‐development and consumption, and therefore, in urgent need of protection. Elicitation is considered to be an effective strategy to enhance the secondary metabolites in plant cell and organ cultures. Secondary metabolite, signal molecules, and gene expression in adventitious roots were studied by HPLC‐ESI‐MSn, commercially available kits and qRT‐PCR method, respectively. In the present study, with the addition of linolenic acid, linoleic acid, and Pichia pastoris, the highest concentration of metabolites was achieved by P. pastoris treatment. The contents of total flavonoids (7.16 mg/g) and polysaccharide (149.76 mg/g) peaked at 100 mg/L of P. pastoris, which increased by 3.09‐fold and 3.28‐fold compared with the control, respectively. However, the highest concentration of glycyrrhizic acid (0.62 mg/g) and glycyrrhetinic acid (0.29 mg/g) were obtained in 200 mg/L of P. pastoris and which were 3.89‐fold and 2.42‐fold more than the control group, respectively. ESI‐MSn analysis indicated that licoricesaponine B2, licoricesapoine G2, licoricesaponine J2, ononin, uralenin, gancaonin C were only identified in the P. pastoris treatment group. Furthermore, P. pastoris also enhanced accumulation of salicylic acid, jasmonic acid, nitric oxide and activities of antioxidant enzymes involved in the plant defense response. In addition, the transcriptional activity of genes involved in glycyrrhizic acid biosynthesis was significantly increased under the treatment of P. pastoris. The results provided a scientific evidence for the further exploitation of G. uralensis adventitious roots and clinical medication. Practical applications This study provided an effective strategy to enhance metabolites by Pichia pastoris treatment in adventitious roots of G. uralensis. The data provide a scientific evidence for the further exploitation of G. uralensis adventitious roots and clinical medication.

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“…Adventitious (AD) root cultures give best results for the production of bioactive compounds such as mother plants [14]. Furthermore, it has been found that bioactive compounds mostly accumulate in roots of various plants [15].…”

Section: Introductionmentioning

confidence: 99%

Stimulation of secondary metabolites by copper and gold nanoparticles in submerge adventitious root cultures of Stevia rebaudiana (Bert.)

Ghazal

Saif

Farid

et al. 2018

IET nanobiotechnol.

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Nanotechnology is one of the advance technologies that almost found implications in every field of science. The importance is due to the unique properties of nanoparticles. In this study, bimetallic alloys of copper (Cu) and gold (Au) were tested in submerge root cultures of for production of biomass and secondary metabolites. A known amount of inoculum roots were submerged in liquid Murashige and Skoog medium containing combination of naphthalene acetic acid (NAA; 0.5 mg l) and different ratios of nanoparticles (NPs). NAA augmented medium was used as control. The addition of nanoparticles (30 µg l) stimulated biomass accumulation (1.447 g/flask) on 27th day of log phases. The maximum total phenolics content (TPC; 16.17 mg/g-DW) and total flavonoids content (TFC; 4.20 mg/g-DW) were displayed using AuCu-NPs (1:3) and NAA. The same combinations enhanced total phenolic production (TPP; 116 mg/L) and total flavonoid production (TFP; 29.5 mg/L) in submerged cultures. A strong correlation was observed between phenolics, flavonoids and dry biomass. Moreover, maximum 1, 1-diphenyl-2-picrylhydrazyl (DPPH) activity of 79% was displayed by addition of AuCu (1:3) nanoparticles. In conclusion, nanoparticles application has shown a positive effect in enhancing biomass and secondary metabolites production in adventitious root cultures of .

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Establishment of adventitious root cultures from leaf explants of Plumbago rosea and enhanced plumbagin production through elicitation

Cited by 35 publications

References 49 publications

Elicitation-Based Method for Increasing the Production of Antioxidant and Bactericidal Phenolic Compounds in Dionaea muscipula J. Ellis Tissue

Elicitation-Based Method for Increasing the Production of Antioxidant and Bactericidal Phenolic Compounds in Dionaea muscipula J. Ellis Tissue

Induction of signal molecules and expression of functional genes after Pichia pastoris stimulation in Glycyrrhiza uralensis Fisch adventitious roots

Stimulation of secondary metabolites by copper and gold nanoparticles in submerge adventitious root cultures of Stevia rebaudiana (Bert.)

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