Establishment of an Animal Model of Disk Degeneration by Intradiskal Injection of Monosodium Iodoacetate

Elmounedi, Najah; Bahloul, Walid; Guidara, Ahmed Racem; Aoui, Mourad; Trigui, Mœz; Keskes, Hassib

doi:10.1016/j.wneu.2023.02.089

Cited by 3 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Macroscopic and histologic examinations stand for classic methods to verify DD. The distorted and damaged area of AF, the decrease of cell number in the NP, and the disruption of the limit between NP and AF help assess the degree of disc degeneration [ 14 , 15 ]. Moreover, the increased expression of collagen I is another sign of DD [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Original animal model of lumbar disc degeneration

Elmounedi

Bahloul

Aoui

et al. 2023

Libyan Journal of Medicine

Self Cite

View full text Add to dashboard Cite

Purpose: Disc degeneration (DD) is a common cause of low back pain, which represents one of the most widespread public health problems in the world. Therefore, the establishment of a reproducible animal model is indispensable to understand the pathogenic mechanisms of DD and to test new therapeutic strategies. From this perspective, the fundamental objective of this study was to elucidate the effect of ovariectomy in establishing a new animal model of DD in rats. Methods: 36 female Sprague-Dawley rats were divided into four groups of 9 rats: Group 1: Negative control (Sham): Only an abdominal skin incision and sutures were performed. Group 2: Ovariectomy (OVX): Removal of two ovaries through a transverse incision in the middle of the abdomen. Group 3: Puncture (Punct): Puncture of lumbar intervertebral discs (L3/4, L4/5, and L5/6) by a 21 G needle. Group 4: Puncture+ovariectomy (Punct+OVX): Removal of two ovaries and puncture of L3/4, L4/5, and L5/6 discs. The rats were euthanized 1, 3, and 6 weeks post-surgery, and the discs were harvested. Validity was assessed by radiography, histology, and biochemistry (water content). Results: Disc height, water content, and histologic score decreased significantly in the last 3 groups and at all three-time points ( P < 0.05). DD progressed over time in the Punct and Punct+OVX groups ( P < 0.05). The changes were more severe in the Punct+OVX group compared to the Punct group and the OVX group. Conclusion: The combination of puncture and ovariectomy induced rapid and progressive DD in the lumbar discs of rats without spontaneous recovery.

show abstract

Section: Discussionmentioning

confidence: 99%