Establishment of an animal model for delayed-onset muscle soreness after high-intensity eccentric exercise and its application for investigating the efficacy of low-load eccentric training

Munehiro, Teppei; Kitaoka, Katsuhiko; Ueda, Yoshimichi; Maruhashi, Yoshinobu; Tsuchiya, Hiroyuki

doi:10.1007/s00776-012-0212-1

Cited by 9 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, allopurinol, an inhibitor of xanthine oxidase, has been shown to have antinociceptive properties in mice [47]. Thus, these results support the hypothesis that xanthine oxidase may also be involved in the onset of DOMS [15,20,37].…”

Section: Discussionsupporting

confidence: 76%

“…In parallel, it is well known that increased levels of uric acid generated by the xanthine oxidase pathway play a pivotal role in the epidemiology of gout disease because of the deposition of uric acid crystals in the cartilage of the joints [29]. Considering that eccentric contractions are often associated with skeletal muscle ROS production and that xanthine oxidase enhances ROS production under hypoxic conditions, it is plausible to propose that ROS may be involved in eccentric exercise-related DOMS [2,21,37].…”

Section: Introductionmentioning

confidence: 97%

“…Regarding DOMS, a considerable body of evidence suggests that the generation of reactive oxygen species (ROS) after strenuous exercise results in muscle damage, which may be involved in postexercise DOMS [2,37]. In this context, it has been demonstrated that exercises requiring over 50% of the voluntary contraction of the muscle may cause a local blood flow reduction [57], which could lead to performance impairment [31].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Increased xanthine oxidase-related ROS production and TRPV1 synthesis preceding DOMS post-eccentric exercise in rats

et al. 2016

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Increased xanthine oxidase-related ROS production and TRPV1 synthesis preceding DOMS post-eccentric exercise in rats

et al. 2016

View full text Add to dashboard Cite

“…The exercise training protocol has been described previously (Ogura et al 2011;Munehiro et al 2012;Lewis et al 2013;Chavanelle et al 2014). In the first week of the experiment, rats were adapted to the treadmill (Omnipacer model LC-4; Omnitech, Columbus, OH, USA).…”

Section: Physical Training Protocolmentioning

confidence: 99%

Gradual downhill running improves age‐related skeletal muscle and bone weakness: implication of autophagy and bone morphogenetic proteins

Kim

Lee

2016

Experimental Physiology

View full text Add to dashboard Cite

Edited by: Philip Atherton New Findings r What is the central question of this study?Exercise training by running has an effect on age-related muscle and bone wasting that improves physical activity and quality of life in the elderly. However, the effect of downhill running on age-related muscle and bone wasting, and its mechanisms, are unclear. r What is the main finding and its importance?Gradual downhill running can improve skeletal muscle growth and bone formation by enhancing autophagy and bone morphogenetic protein signalling in aged rats. Therefore, downhill running exercise might be a practical intervention to improve skeletal muscle and bone protection in the elderly.Recent evidence suggests that autophagy and the bone morphogenetic protein (BMP) signalling pathway regulate skeletal muscle growth and bone formation in aged rats. However, the effect of downhill running on muscle growth and bone formation is not well understood. Thus, we investigated the effect of downhill and uphill running on age-related muscle and bone weakness. Young and late middle-aged rats were randomly assigned to control groups (young, YC; and late middle-aged, LMC) and two types of running training groups (late middle-aged downhill, LMD; and late middle-aged uphill, LMU). Training was progressively carried out on a treadmill at a speed of 21 m min −1 with a slope of +10 deg for uphill training versus 16 m min −1 with a slope of −16 deg for downhill training, both for 60 min day −1 , 5 days week −1 for 8 weeks. Downhill and uphill training increased autophagy-related protein 5, microtubule-associated protein light chain, Beclin-1 and p62 proteins in aged rats. In addition, superoxide dismutase, haem oxygenase-1 and the BMP signalling pathway were elevated. Phosphorylation of mammalian target of rapamycin and myogenic differentiation were increased significantly in the LMD and LMU groups. Consequently, in the femur, BMP-2, BMP-7 and autophagy molecules were highly expressed in the LMD and LMU groups. These results suggest that both downhill and uphill training appear to have a positive effect on expression of autophagy molecules and BMPs. In particular, these physiological adaptations from gradual downhill exercise have an effect on bone morphological changes and muscle quality similar to gradual uphill training interventions in ageing.

show abstract

“…Downhill (DH) running is often used as a model of eccentric exercise in rats to examine skeletal muscle damage/regeneration processes following eccentric‐induced mechanical stress . However, fewer studies have focused on eccentric training‐induced metabolic adaptations in rats, especially on skeletal muscle aerobic energy production in mitochondria .…”

mentioning

confidence: 99%

Mitochondrial function following downhill and/or uphill exercise training in rats

et al. 2016

View full text Add to dashboard Cite

show abstract

Establishment of an animal model for delayed-onset muscle soreness after high-intensity eccentric exercise and its application for investigating the efficacy of low-load eccentric training

Cited by 9 publications

References 23 publications

Increased xanthine oxidase-related ROS production and TRPV1 synthesis preceding DOMS post-eccentric exercise in rats

Increased xanthine oxidase-related ROS production and TRPV1 synthesis preceding DOMS post-eccentric exercise in rats

Gradual downhill running improves age‐related skeletal muscle and bone weakness: implication of autophagy and bone morphogenetic proteins

Mitochondrial function following downhill and/or uphill exercise training in rats

Contact Info

Product

Resources

About