Establishment of an ascitic human melanoma cell line that metastasizes to lung and liver in nude mice

Abstract

UCLA-SO-M14, a human melanoma cell line, was cultured for ten passages in vitro. The line was converted to an ascitic form, M14-A, by transplanting M14 into CD-1 nude mice and back into tissue culture. The minimum number of M14-A cells that formed ascites in all mice (within 10-21 days) was 5 X 10(5), and over 80% of such mice developed macroscopic liver metastases. M14-A inoculated subcutaneously formed tumor at the site of injection, but rarely led to the development of metastases. However, when M14-A was inoculated subcutaneously in young mice (1-14 days old), more than 50% developed lung (but not liver) metastases. The reproducibility of the formation of ascites and metastases was confirmed by testing M14-A at various passages. M14-A may be useful as a model for the metastatic process in human melanoma.

Metastatic behavior of human melanoma cell lines in nude mice correlates with urokinase-type plasminogen activator, its type-1 inhibitor, and urokinase-mediated matrix degradation.

“…u-PA was in the MV3 and BLM cells partly found in a cell-associated form (Table I) . This portion of urokinase can either be located intracellularly or at the cell surface, specifically bound to the urokinase receptor (31,45,46,55,62) . The presence of a specific u-PA receptor in all the melanoma cell cultures was demonstrated by cross-linking experiments with 125 1 labeled DFP-treated urokinase.…”

“…The BLM cell line is a subline of BRO (35) and was isolated from lung metastases after subcutaneous inoculation of nude mice with BRO cells . The M14 (31) …”

Regression of cutaneous metastatic melanoma by intralesional injection with human monoclonal antibody to ganglioside GD2.

“…After testing the antibody titer by the immune adherence assay (1), spent medium from each culture was pooled and centrifuged at 10,000 x g for 20 min to remove cell debris. Ten liters of cell-free spent medium from the L72 culture was processed at one time for antibody purification as described (3). The purified human IgM was concentrated to 5 mg/ml, filtrated through a 0.…”

“…The antibody has a strong cytotoxic effect on human melanoma cells in the presence of complement (3). Its in vivo anti-tumor effects were demonstrated by using the athymic nude mouse model (4,5).…”

A novel three-dimensional model to quantify metastatic melanoma invasion

“…22) was also cultured within the system to contrast with the invasion displayed by the highly metastatic C8161.9. The sporadically metastatic M14 assumed a coordinated form of invasion (Fig.…”