Establishment of an Ex Vivo Lung Perfusion Model Using Non-Heart-Beating Large Pigs

Okamoto, Toshihiro; Chen, F.; Zhang, J.; Yamada, Tetsu; Nakayama, Eiichiro; Morikawa, Hiromasa; Bando, Toru; Date, Hiroshi

doi:10.1016/j.transproceed.2010.03.140

Cited by 11 publications

(5 citation statements)

References 16 publications

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“…Other groups have successfully conducted perfusion experiments with slaughterhouse organs including kidneys, 14,49 livers, 50,51 hearts, 15,52 and lungs. 12,13,53 …”

Section: Discussionmentioning

confidence: 99%

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Comparison of normothermic and hypothermic perfusion in porcine kidneys donated after cardiac death

Blum

Liu

Soliman

et al. 2017

Journal of Surgical Research

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Background Normothermic machine perfusion (NMP) is an alternative strategy for preserving kidneys donated after cardiac death (DCD). The relative efficacy of prolonged NMP compared to hypothermic machine perfusion (HMP) in DCD kidneys with moderate ischemic injury is undetermined. This study compares NMP and HMP kidney preservation in a porcine DCD model. Methods Ten porcine kidneys underwent NMP or HMP preservation following 45 minutes of warm ischemia and 5 hours of cold ischemia. After 8 hours of machine preservation, hemodynamic stability, renal function, perfusate biomarkers, and histologic integrity were assessed in a simulated reperfusion model. Results Upon simulated reperfusion, no differences were observed in oxygen consumption, urine production, creatinine clearance, fractional excretion of sodium, proteinuria, and perfusate levels of lactate dehydrogenase and aspartate aminotransferase. Resistance was no different after 30 minutes of simulated reperfusion. NMP kidneys demonstrated increased histologic vacuolization after preservation and greater loss of tubular integrity after simulated reperfusion. Perfusate levels of alkaline phosphatase (AP) and gamma glutamyltransferase (GGT) were higher in NMP kidneys during preservation, but upon simulated reperfusion, AP and GGT levels were higher in HMP-preserved kidneys. Peak AP and GGT during HMP simulated reperfusion were over 14 times higher than peak AP and GGT during NMP preservation. Conclusions NMP provided comparable preservation of renal function as HMP and minimized AP and GGT release upon reperfusion.

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“…Other groups have successfully conducted perfusion experiments with slaughterhouse organs including kidneys, 14,49 livers, 50,51 hearts, 15,52 and lungs. 12,13,53 …”

Section: Discussionmentioning

confidence: 99%

“…11 As previously described in translational experiments, 12–15 animals were rendered unconscious and killed by exsanguination through a carotid artery and jugular vein incision, thus initiating the warm ischemic period. 2 L of blood were collected in a heparinized container (60,000 IU), transferred to citrated blood bags, and stored on ice.…”

Section: Methodsmentioning

confidence: 99%

Comparison of normothermic and hypothermic perfusion in porcine kidneys donated after cardiac death

Blum

Liu

Soliman

et al. 2017

Journal of Surgical Research

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“…Okamoto et al first reported on using porcine abattoir lungs for EVLP. 12 They were successful in establishing perfusion for 2–4 h. We think a crucial difference that enabled them to use pig lungs, is that their supplier did not scald the pigs, which is usually impossible to prevent in the western hemisphere and most of Europe. Unfortunately, they did not present perfusate analyses, go in‐depth regarding ventilatory lung outcomes, nor provided detailed descriptions, making it difficult to compare.…”

Section: Discussionmentioning

confidence: 99%

“…Okamoto et al first reported on using porcine abattoir lungs for EVLP 12 . They were successful in establishing perfusion for 2–4 h. We think a crucial difference that enabled them to use pig lungs, is that their supplier did not scald the pigs, which is usually impossible to prevent in the western hemisphere and most of Europe.…”

Section: Discussionmentioning

confidence: 99%

Establishing an economical and widely accessible donation after circulatory death animal abattoir model for lung research using ex vivo lung perfusion

Zhang

Moeslund

et al. 2022

Artificial Organs

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Background Ex vivo lung perfusion (EVLP), is a platform that allows simultaneous testing and treatment of the lungs. However, use of EVLP is costly and requires access to lab animals and accompanying facilities. To increase the use of EVLP for research, we developed a method to perform EVLP using abattoir procured lungs. Furthermore, we were also able to significantly decrease costs. Methods Six pair of lungs were procured from abattoir sheep. The lungs were then flushed and stored in ice for 3 h. A low‐flow (20% of cardiac output) approach, a tidal volume of 6 ml/kg bodyweight and total perfusion time of 3 h were chosen. Perfusion fluids and circuits were self‐made. Lung biopsies, perfusate collection, respiratory values, circulatory pressures were recorded and hourly blood gas analyses were performed. Results Mean pO2 remained stable from 60 min (49.3 ± 7.1 kPa) to 180 min (51.5 kPa ± 8.0), p = 0.66. Pulmonary artery pressure remained ≤15 mm Hg and the left atrial pressure remained between 3 and 5 mm Hg and peak respiratory pressures ≤20 cmH2O. Lactate dehydrogenase increased from start (96.3 ± 56.4 U/L) to the end of perfusion (315.8 ± 85.0 U/L), p < 0.05. No difference was observed in ATP between procurement and post‐EVLP, 129.7 ± 37.4 μmol/g protein to 132.0 ± 23.4 μmol/g, p = 0.92. Conclusions Sheep lungs, acquired from an abattoir, can be ex vivo perfused under similar conditions as lab animal lungs with similar results regarding e.g., oxygenation and ATP restoration. Furthermore, costs can be significantly reduced by making use of this abattoir model. By increasing accessibility and lowering costs for experiments using lung perfusion, more results may be achieved in the field of lung diseases.

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“…Systems of this type are object of concurrent research in the field of live organs transplantation (Aigner et al, 2012) Organs from cadaver donor, especially lungs, must be carefully treated before transplantation to prevent irreversible functional changes which can occur during transport from the donor to the recipient. The same is valid when we want to perform some ex-vivo experiments (Wallinder, 2014), (Okamoto et al, 2010). MCM is in fact designed to provide sterile and reliable ex-vivo perfusion of lungs.…”

Section: Introductionmentioning

confidence: 88%

Mathematical model of Mobile Circulatory Module for ex-vivo lungs perfusion

2015

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The article deals with mathematical modelling of a system for ex-vivo lung perfusion. The system which is modelled here is named Mobile Circulatory Module and is designed for transport of live lungs namely porcine or human lungs. Transported lungs should be used for various research or even for transplantation. The article describes mathematical model of thermal circuit and hydraulic circuit which are vital parts of the MCM to secure living conditions of the transported organ. Mathematical model is simulated with MATLAB Simulink and shows good qualitative correspondence with real behavior of the system. Based on math model there is designed control algorithm for temperature control and there is discussed control of perfusion liquid as well.

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Establishment of an Ex Vivo Lung Perfusion Model Using Non-Heart-Beating Large Pigs

Cited by 11 publications

References 16 publications

Comparison of normothermic and hypothermic perfusion in porcine kidneys donated after cardiac death

Comparison of normothermic and hypothermic perfusion in porcine kidneys donated after cardiac death

Establishing an economical and widely accessible donation after circulatory death animal abattoir model for lung research using ex vivo lung perfusion

Mathematical model of Mobile Circulatory Module for ex-vivo lungs perfusion

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