Establishment of an experimental model of normal dog bladder organoid using a three-dimensional culture method

Elbadawy, Mohamed; Fujisaka, Kodai; Yamamoto, Hajime; Tsunedomi, Ryouichi; Nagano, Hiroaki; Ayame, Hiromi; Ishihara, Yusuke; Mori, Takashi; Azakami, Daigo; Uchide, Tsuyoshi; Fukushima, Ryuji; Abugomaa, Amira; Kaneda, Masahiro; Yamawaki, Hideyuki; Shinohara, Yuta; Omatsu, Tsutomu; Mizutani, Tetsuya; Usui, Tatsuya; Sasaki, Kenji

doi:10.1016/j.biopha.2022.113105

Cited by 18 publications

(15 citation statements)

References 81 publications

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“…Uroplakin (UPK) proteins are specific to urothelial cells (Lu et al, 2022), and both Uroplakin 2 (UPK2, FWER = 1.70 × 10 -2 ) and Uroplakin 3A (UPK3A, FWER = 4.74 × 10 -3 ) were upregulated in organoids ( Figure 2B ). UPK3A is an umbrella cell marker, while Keratin 5 (KRT5) is indicative of basal cells and was bladder-specific and upregulated in the canine bladder organoids (Elbadawy et al, 2022; Kim et al, 2020a). The bladder-specific markers PFN1 and EIF4A1 were expressed in both organoids and tissues.…”

Section: Resultsmentioning

confidence: 99%

“…Since canine bladder cancer is a well-established model for human muscle-invasive bladder cancer (Knapp et al, 2020), canine bladder cancer organoids represent a valuable model for translational preclinical research (Minkler et al, 2021). In addition, Elbadawy et al has recently described healthy canine bladder organoids (Elbadawy et al, 2022). This report expands the knowledge and accessibility of healthy canine bladder organoids, with those described here displaying a similar morphological phenotype to those previously described ( Figure 1 ) (Elbadawy et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, Elbadawy et al has recently described healthy canine bladder organoids (Elbadawy et al, 2022). This report expands the knowledge and accessibility of healthy canine bladder organoids, with those described here displaying a similar morphological phenotype to those previously described ( Figure 1 ) (Elbadawy et al, 2022). Using 3D patient-derived tumor organoids to predict the response to chemotherapeutic protocols has great potential in oncological precision medicine.…”

Section: Discussionmentioning

confidence: 99%

“…This report describes the successful cultivation of three novel canine organoid lines of which none are published in a peer-reviewed journal, including the endometrium, lung, and pancreas, in addition to previously described kidney (Chen et al, 2019), bladder (Elbadawy et al, 2022), and liver (Nantasanti et al, 2015) organoids from two related dogs. By comparing six tissue-specific organoid lines obtained from two genetically related donors, this study aims to acquire insight into gene expression in different organoids and their corresponding tissues.…”

Section: Introductionmentioning

confidence: 99%

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A Multi-Tissue Comparison and Molecular Characterization of Canine Organoids

Zdyrski

Gabriel

Ospina

et al. 2022

Preprint

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Organoids are 3-dimensional (3D) stem cell-derived cell culture lines that offer a variety of technical advantages compared to traditional 2-dimensional (2D) cell cultures. Although murine models have proved useful in biomedical research, rodent models often fail to adequately mimic human physiology and disease progression, resulting in poor preclinical prediction of therapeutic drug efficacy and toxicity. With the advent of organoid technology, many of these challenges can be overcome. Previously, the use of canine organoids in drug testing and disease modeling was limited to organoids originating from the intestine, liver, kidney, and urinary bladder. Here, we report the cultivation, maintenance, and molecular characterization of three novel adult-stem cell-derived canine organoid cell lines, including the endometrium, lung, and pancreas, in addition to previously reported kidney, bladder, and liver organoids from two genetically related canines. Six tissues and organoid lines from each donor were characterized using bulk RNASeq, allowing for a unique, multi-organ comparison between these two individuals and identification of specific cell types such as glandular epithelial cells in endometrial organoids.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Multi-Tissue Comparison and Molecular Characterization of Canine Organoids

Zdyrski

Gabriel

Ospina

et al. 2022

Preprint

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“…Currently, very few organoid biobanks of bladder cancer are established. Researchers have successfully generated normal and neoplastic organoids from dog urine samples 94 , 95 .…”

Section: Overview Of Pdto Biobanksmentioning

confidence: 99%

Tumor organoid biobank-new platform for medical research

Song

2023

Sci Rep

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Organoids are a new type of 3D model for tumor research, which makes up for the shortcomings of cell lines and xenograft models, and promotes the development of personalized precision medicine. Long-term culture, expansion and storage of organoids provide the necessary conditions for the establishment of biobanks. Biobanks standardize the collection and preservation of normal or pathological specimens, as well as related clinical information. The tumor organoid biobank has a good quality control system, which is conducive to the clinical transformation and large-scale application of tumor organoids, such as disease modeling, new drug development and high-throughput drug screening. This article summarized the common tumor types of patient-derived organoid (PDO) biobanks and the necessary information for biobank construction, such as the number of organoids, morphology, success rate of culture and resuscitation, pathological types. In our results, we found that patient-derived tumor organoid (PDTO) biobanks were being established more and more, with the Netherlands, the United States, and China establishing the most. Biobanks of colorectal, pancreas, breast, glioma, and bladder cancers were established more, which reflected the relative maturity of culture techniques for these tumors. In addition, we provided insights on the precautions and future development direction of PDTO biobank building.

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A systematic review on the culture methods and applications of 3D tumoroids for cancer research and personalized medicine

Kalla,

Pfneissl,

Mair

et al. 2024

Cell Oncol.

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Cancer is a highly heterogeneous disease, and thus treatment responses vary greatly between patients. To improve therapy efficacy and outcome for cancer patients, more representative and patient-specific preclinical models are needed. Organoids and tumoroids are 3D cell culture models that typically retain the genetic and epigenetic characteristics, as well as the morphology, of their tissue of origin. Thus, they can be used to understand the underlying mechanisms of cancer initiation, progression, and metastasis in a more physiological setting. Additionally, co-culture methods of tumoroids and cancer-associated cells can help to understand the interplay between a tumor and its tumor microenvironment. In recent years, tumoroids have already helped to refine treatments and to identify new targets for cancer therapy. Advanced culturing systems such as chip-based fluidic devices and bioprinting methods in combination with tumoroids have been used for high-throughput applications for personalized medicine. Even though organoid and tumoroid models are complex in vitro systems, validation of results in vivo is still the common practice. Here, we describe how both animal- and human-derived tumoroids have helped to identify novel vulnerabilities for cancer treatment in recent years, and how they are currently used for precision medicine.

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Establishment of an experimental model of normal dog bladder organoid using a three-dimensional culture method

Cited by 18 publications

References 81 publications

A Multi-Tissue Comparison and Molecular Characterization of Canine Organoids

A Multi-Tissue Comparison and Molecular Characterization of Canine Organoids

Tumor organoid biobank-new platform for medical research

A systematic review on the culture methods and applications of 3D tumoroids for cancer research and personalized medicine

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