Establishment of an Open, Sugar-Free Tissue Culture System for Sugarcane Micropropagation

Lü, Jiaju; Ali, Ahmad; He, Er‐Qi; Arak, Tira-umphon; Gao, San‐Ji

doi:10.1007/s12355-019-00758-1

Cited by 5 publications

(2 citation statements)

References 21 publications

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“…When shoots reached 30 ± 5 mm in length, they were then passaged and cultured for 20 days. Subsequently, 25–30 mm long seedlings were transferred to the rooting medium for 25 d and then subsequently colonized in fields [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Can endophytic microbial compositions in cane roots be shaped by different propagation methods

et al. 2023

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In practical production, cane stems with buds are generally used as seed for propagation. However, long-terms cane stems only easily lead to some problems such as disease sensitivity, quality loss, etc. Recently, cane seedings, which are produced by tissue culture were used in sugarcane production, but few studies on cane health related to tissue culture seedings. Therefore, to evaluate the immunity and health of sugarcanes growing from different reproduction modes, the endophytic microbial compositions in cane roots between stem and tissue culture seedlings were analyzed using high-throughput techniques. The results showed that the endophytic microbial compositions in cane roots were significant differences between stem and tissue culture seedlings. At the genus level, Pantoea, Bacillus, Streptomyces, Lechevalieria, Pseudomonas, Nocardioides, unclassified_f__Comamonadaceae enriched as the dominant endophytic bacterial genera, and Rhizoctonia, Sarocladium, Scytalidium, Wongia, Fusarium, unclassified_f__Phaeosphaer, unclassified_c__Sordariom, unclassified_f__Stachybot, Poaceascoma, Microdochium, Arnium, Echria, Mycena and Exophiala enriched as the dominant endophytic fungal genera in cane roots growing from the tissue culture seedlings. In contrast, Mycobacterium, Massilia, Ralstonia, unclassified_f__Pseudonocardiacea, norank_f__Micropepsaceae, Leptothrix and Bryobacter were the dominant endophytic bacterial genera, and unclassified_k__Fungi, unclassified_f__Marasmiaceae, Talaromyces, unclassified_c__Sordariomycetes and Trichocladium were the dominant endophytic fungal genera in cane roots growing from stem seedlings. Additionally, the numbers of bacterial and fungal operational taxonomic units (OTUs) in cane roots growing from tissue culture seedlings were significantly higher than those of stem seedlings. It indicates that not only the endophytic microbial compositions in cane roots can be shaped by different propagation methods, but also the stress resistance of sugarcanes can be improved by the tissue culture propagation method.

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Section: Methodsmentioning

confidence: 99%

Can endophytic microbial compositions in cane roots be shaped by different propagation methods

et al. 2023

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show abstract

“…In addition, various research have examined salt-tolerant plants derived from calluses, such as wheat ( Barakat and Abdel-Latif, 1996 ), rice ( Revathi and Pillai, 2015 ), pomegranate ( El Mahdy et al, 2022 ) and sugarcane ( Gandonou et al, 2005 , Shomeili et al, 2011 ). This technique is simple, easy to use, and highly reproducible ( Lal et al, 2015 , Lu et al, 2020 , El–Mahdy et al, 2022 ). However, the molecular, biochemical, and physiological characteristics of the selected clones have not been extensively assessed.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo screening for the identification of salt-tolerant sugarcane (Saccharum officinarum L.) clones: molecular, biochemical, and physiological responses to salt stress

Laksana

Sophiphun

Chanprame

2023

Saudi Journal of Biological Sciences

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Commercial Micropropagation of Some Economically Important Crops

Bhatt

2021

Agricultural Biotechnology: Latest Research and Trends

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Establishment of an Open, Sugar-Free Tissue Culture System for Sugarcane Micropropagation

Cited by 5 publications

References 21 publications

Can endophytic microbial compositions in cane roots be shaped by different propagation methods

Can endophytic microbial compositions in cane roots be shaped by different propagation methods

In vitro and in vivo screening for the identification of salt-tolerant sugarcane (Saccharum officinarum L.) clones: molecular, biochemical, and physiological responses to salt stress

Commercial Micropropagation of Some Economically Important Crops

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