Establishment of an R-banded rabbit karyotype nomenclature by FISH localization of 23 chromosome-specific genes on both G- and R-banded chromosomes

Hayes, Hélène; Rogel–Gaillard, Claire; Zijlstra, C.; Haan, N.A. de; Urien, Céline; Bourgéaux, Noëlle; Bertaud, Maud; Bosma, A. A.

doi:10.1159/000069807

Cited by 36 publications

(31 citation statements)

References 14 publications

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“…All rabbit bacterial artificial chromosomes (BACs) were isolated from a rabbit library maintained at the UR1313, Unité de Génétique Animale et Biologie Intégrative, Institut National de la Recherché Agronomique (INRA). Hprt1 and Jarid1c BACs had been previously published 35,36 . Xist-containing BACs (573D5, 614B8 and 717B2) were isolated by a PCR-based screening protocol (C. Rogel-Gaillard, unpublished results).…”

Section: Methods Summarymentioning

confidence: 99%

Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development

Okamoto

Patrat

Thépot

et al. 2011

Nature

423

458

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X-chromosome inactivation (XCI) in female mammals allows dosage compensation for X-linked gene products between the sexes1. The developmental regulation of this process has been extensively investigated in mice, where the X chromosome of paternal origin (Xp) is silenced during early embryogenesis owing to imprinted expression of the regulatory RNA, Xist (X-inactive specific transcript). Paternal XCI is reversed in the inner cell mass of the blastocyst and random XCI subsequently occurs in epiblast cells. Here we show that other eutherian mammals have very different strategies for initiating XCI. In rabbits and humans, the Xist homologue is not subject to imprinting and XCI begins later than in mice. Furthermore,Xist is upregulated on both X chromosomes in a high proportion of rabbit andhuman embryo cells, even in the inner cell mass. In rabbits, this triggers XCI on both X chromosomes in some cells. In humans, chromosome-wide XCI has not initiated even by the blastocyst stage, despite the upregulation of XIST. The choice of which X chromosome will finally become inactive thus occurs downstream of Xist upregulation in both rabbits and humans, unlike in mice. Our study demonstrates the remarkable diversity in XCI regulation and highlights differences between mammals in their requirement for dosage compensation during early embryogenesis

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Section: Methods Summarymentioning

confidence: 99%

Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development

Okamoto

Patrat

Thépot

et al. 2011

Nature

423

458

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“…Our data on the rabbit SRY suggests that this sex-determining gene is probably duplicated and evolving under concerted evolution in Lagomorphs. The recent identification of a clone that contains this gene [8] in a rabbit BAC library [13] will hopefully allow its complete sequencing in our laboratories and the examination of the hypotheses described above.…”

Section: Resultsmentioning

confidence: 99%

A 7-bp insertion in the 3' untranslated region suggests the duplication and concerted evolution of the rabbitSRYgene

Geraldes

Ferrand

2006

Genet. Sel. Evol.

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-In this work we report the genetic polymorphism of a 7-bp insertion in the 3' untranslated region of the rabbit SRY gene. The polymorphic GAATTAA motif was found exclusively in one of the two divergent rabbit Y-chromosomal lineages, suggesting that its origin is more recent than the separation of the O. c. algirus and O. c. cuniculus Y-chromosomes. In addition, the remarkable observation of haplotypes exhibiting 0, 1 and 2 7-bp inserts in essentially all algirus populations suggests that the rabbit SRY gene is duplicated and evolving under concerted evolution. rabbit / SRY gene / polymorphic insertion / duplication / concerted evolution

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“…To this end, we performed a FISH analysis using 2 BAC clones as specific probes, either for the rabbit X chromosome (530F8 at Xq26.1) or for the rabbit Y chromosome (828D7 at Yp12) [27]. BAC 530F8 was labeled with Spectrum Green, and BAC 828D7 with Spectrum Red (all from Vysis) by a standard nick translation reaction.…”

Section: Fluorescent In Situ Hibridizationmentioning

confidence: 99%

Fetal Liver-Derived Mesenchymal Stem Cell Engraftment After Allogeneic In Utero Transplantation into Rabbits

Moreno

Martínez-González²,

Rosal³

et al. 2012

Stem Cells and Development

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Prenatal transplantation of genetically engineered mesenchymal stem cells (MSCs) might benefit prevention or treatment of early-onset genetic disorders due to the cells' intrinsic regenerative potential plus the acquired advantage from therapeutic transgene expression. However, a thorough assessment of the safety, accessibility, and behavior of these MSCs in the fetal environment using appropriate animal models is required before we can advance toward a clinical application. We have recently shown that fetal rabbit liver MSCs (fl-MSCs) have superior growth rate, clonogenic capability, and in vitro adherence and differentiation abilities compared with adult rabbit bone marrow MSCs. In this follow-up study, we report safe and widespread distribution of recombinant pSF-EGFP retrovirus-transduced fl-MSCs (EGFP + -fl-MSCs) in neonatal rabbit tissues at 10 days after fetal allogeneic transplantation through both intrahepatic and intra-amniotic administration. Conversely, a more restricted biodistribution pattern according to the route of administration was apparent in the young rabbits intervened at 16 weeks after fetal EGFP + -fl-MSC transplantation. Furthermore, the presence of these cells in the recipients' tissues, tracked with the reporter provirus, was inversely related to the developmental stage of the fetuses at the time of intervention. Long-term engraftment was confirmed both by fluorescence in situ hybridization analysis on touch tissue imprints using a chromosome Y-specific BAC probe, and by immunohistochemical localization of EGFP expression. Finally, there was no evidence of immune responses against the transplanted EGFP + -fl-MSCs or the EGFP transgenic product in the treated young rabbits. Thus, cell transplantation approaches using genetically engineered fetal MSCs may prove particularly valuable to frontier medical treatments for congenital birth defects in perinatology.

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Establishment of an R-banded rabbit karyotype nomenclature by FISH localization of 23 chromosome-specific genes on both G- and R-banded chromosomes

Cited by 36 publications

References 14 publications

Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development

Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development

A 7-bp insertion in the 3' untranslated region suggests the duplication and concerted evolution of the rabbitSRYgene

Fetal Liver-Derived Mesenchymal Stem Cell Engraftment After Allogeneic In Utero Transplantation into Rabbits

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