Establishment of angiotensin-converting enzyme 2 and cluster of differentiation 147 dual target cell membrane chromatography based on SNAP-tag technology for screening anti severe acute respiratory syndrome coronavirus 2 active components

Wang, Hong; Jia, Qi; Feng, Jingting; Miao, Chenyang; Ding, Yifan; Liu, Sihan; Feng, Chao; Lv, Yanni; Jing, Huang; Han, Shengli

doi:10.1016/j.chroma.2023.463903

Cited by 8 publications

(2 citation statements)

References 30 publications

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“…Tan, an active constituent of the Chinese medicine Chen Pi, exhibits a diverse range of pharmacological activities ( Cheng et al, 2023 ). Research has demonstrated its potential as an antiviral agent by hindering the entry of the SARS-CoV-2 virus into cells (H. Wang et al, 2023 ). In this study, we elucidated the protective mechanism of Tan against pulmonary fibrosis.…”

Section: Discussionmentioning

confidence: 99%

Tangeretin attenuates bleomycin-induced pulmonary fibrosis by inhibiting epithelial-mesenchymal transition via the PI3K/Akt pathway

Li,

Wei,

Song

et al. 2023

Front. Pharmacol.

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Background: Pulmonary fibrosis (PF) is a terminal pathological change in a variety of lung diseases characterized by excessive deposition of extracellular matrix, for which effective treatment is lacking. Tangeretin (Tan), a flavonoid derived from citrus, has been shown to have a wide range of pharmacological effects. This study aimed to investigate the role and potential mechanisms of Tan on pulmonary fibrosis.Methods: A model of pulmonary fibrosis was established by administering bleomycin through tracheal drip, followed by administering Tan or pirfenidone through gavage. HE and Masson staining were employed to assess the extent of pulmonary fibrosis. Subsequently, Western blot, enzyme-linked immunosorbent assay (ELISA), RNA sequencing, and immunohistochemistry techniques were employed to uncover the protective mechanism of Tan in PF mice. Furthermore, A549 cells were stimulated with TGF-β1 to induce epithelial-mesenchymal transition (EMT) and demonstrate the effectiveness of Tan in mitigating PF.Results: Tan significantly ameliorated bleomycin-induced pulmonary fibrosis, improved fibrotic pathological changes, and collagen deposition in the lungs, and reduced lung inflammation and oxidative stress. The KEGG pathway enrichment analysis revealed a higher number of enriched genes in the PI3K/Akt pathway. Additionally, Tan can inhibit the EMT process related to pulmonary fibrosis.Conclusion: Taken together, the above research results indicate that Tan suppresses inflammation, oxidative stress, and EMT in BLM-induced pulmonary fibrosis via the PI3K/Akt pathway and is a potential agent for the treatment of pulmonary fibrosis.

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Section: Discussionmentioning

confidence: 99%

Tangeretin attenuates bleomycin-induced pulmonary fibrosis by inhibiting epithelial-mesenchymal transition via the PI3K/Akt pathway

Li,

Wei,

Song

et al. 2023

Front. Pharmacol.

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“…By entering the nose and mouth through this protein, it may cause temporary damage to the smell and taste nerves. [5][6][7][8][9][10][11][12] New variants of SARS-CoV-2 began to circulate due to virus mutations. The Omicron variant emerged in November 2021, although new lineages continue to spread globally.…”

mentioning

confidence: 99%

It is necessary to assess olfactory and gustatory function in post covid‐19 patients, due to the omicron variant infection

Machado

Gugierrez‐Gil

González‐Quevedo

2023

Int Forum Allergy Rhinol

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To the Editor:Boscolo-Rizzo et al. 1 concluded that although a proportion of patients recovered from enduring smell/taste dysfunction more than 1 year after coronavirus disease 2019 (COVID-19), some cases still showed a noteworthy remaining olfactory dysfunction 2 years after severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) infection compared to matched normal subjects.Initial reports stated that the most frequent symptoms of COVID-19 infection were fever, cough, dyspnea, and myalgia or fatigue. 2,3 Several authors later described an association between COVID-19 and altered olfactory and taste functions. [4][5][6][7][8] SARS-CoV-2 enters the nasal and mouth tissues through the angiotensin-converting enzyme 2 (ACE2), which is copious in the nose. By entering the nose and mouth through this protein, it may cause temporary damage to the smell and taste nerves. [5][6][7][8][9][10][11][12] New variants of SARS-CoV-2 began to circulate due to virus mutations. The Omicron variant emerged in November 2021, although new lineages continue to spread globally. The SARS-CoV-2 omicron variant has significantly increased COVID-19 diagnoses with less severe clinical manifestations and decreased hospitalizations. The omicron wave has swelled faster than previous waves, completely displacing the delta variant within weeks and creating new worldwide concerns about the ability to achieve final pandemic control. 13,14 The symptoms associated with Omicron differ from "traditional" COVID-19 symptoms, more closely resembling those of the common cold. 15 One major COVID-19 symptom is rare in omicron patients: loss of taste and smell. 16,17 Some research suggested that 48% of people carrying the original mutation of the novel coronavirus lost smell, and 41% had a loss of taste; these numbers decreased to 23% for loss of taste and 12% for loss of smell among omicron-infected patients. 18 Consequently, many people may not realize they have contracted COVID-19 because fewer experience cough, fever, or loss of taste or smell. A virus in the higher respiratory tract is associated with increased transmissibility but with less severe disease, exhibiting similarities with flu strains. 19

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Development of target-based cell membrane affinity ultrafiltration technology for a simplified approach to discovering potential bioactive compounds in natural products

Chen,

Zhou,

et al. 2024

Anal Bioanal Chem

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Establishment of angiotensin-converting enzyme 2 and cluster of differentiation 147 dual target cell membrane chromatography based on SNAP-tag technology for screening anti severe acute respiratory syndrome coronavirus 2 active components

Cited by 8 publications

References 30 publications

Tangeretin attenuates bleomycin-induced pulmonary fibrosis by inhibiting epithelial-mesenchymal transition via the PI3K/Akt pathway

Tangeretin attenuates bleomycin-induced pulmonary fibrosis by inhibiting epithelial-mesenchymal transition via the PI3K/Akt pathway

It is necessary to assess olfactory and gustatory function in post covid‐19 patients, due to the omicron variant infection

Development of target-based cell membrane affinity ultrafiltration technology for a simplified approach to discovering potential bioactive compounds in natural products

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