Establishment of Azorhizobium caulinodans in the rhizosphere of wetland rice ( Oryza sativa L.)

Nieuwenhove, C Van; Holm, Lieven Van; Kulasooriya, S. A.; Vlassak, K.

doi:10.1007/s003740050637

Cited by 35 publications

(13 citation statements)

References 9 publications

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“…Yield responses to inoculation of rhizobacteria also depend on rice cultivars and fertilizer levels. These facts corroborate the findings of Dashti et al (1998), Muthukumarasamy et al (2002) and van Nieuwenhove et al (2000). Rice yield was positively affected by inoculation with Rhizobium strain KR181 and Pseudomonas strain RRj228, which have no detectible N 2 -fixation activity, but have strong plant growth promotion activity at low population concentrations.…”

Section: Discussionsupporting

confidence: 91%

“…Bacillus strains negatively contributed to yield of paddy rice even though they harbored the property of both phytohormon production like IAA (Kang, Unpublished data) and N 2 -fixing activity supposedly indicated that there was an influence of unique environment of submerged paddy soils (Bashan, 1999;van Nieuwenhove et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Especially in the case of rice, incorporation of the endophytic diazotrophs such as Herbaspirillum seropedicae and Burkolderia brasilensis etc., which are capable of inhabiting the stem and leaves of plant, into PGPR inoculum can augment nitrogen to the host plant in temperate as well as tropical regions (Baldani et al, 2001). Despite some successes with the inoculation of plant growth-promoting bacteria, there are still several problems associated with soil texture (Mahaffee, 1991), soil nitrogen (Caballero-Mellado et al, 1995;Muthukumarasamy et al, 2002), soil water (Mayak et al, 2004;Timmusk and Wagner, 1999), and plant growth stage (Ladha et al,1988;van Nieuwenhove et al, 2000;Sethunathan et al, 1983) need to be solved for the establishment of successful association between host plant and PGPR.…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Root Colonization and Evaluation of Some Plant Growth-promoting Rhizobacteria in Paddy Rice

Kang¹,

Park²,

Ko³

et al. 2017

Korean J. Soil. Sci. Fert.

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In order to obtain promising rice growth-promoting microbial strains that can be used as substitutes for chemical fertilizers, 172 bacterial strains were isolated from rice roots grown in Korean and Russian soils. Out of them, the strains KR076, KR083, KR181 and RRj228 showed plant growth-promoting activities on maize seedlings. Bacillus megaterium KR076 and Bacillus sp. KR083 showed both nitrogen-fixing and plant growth-promoting activities, while Rhizobium sp. KR181 and Pseudomonas sp. RRj228 appeared to support only plant growth-promotion, but not N 2 fixation. Especially, RRj228 showed high growth promoting activity at low concentrations. Inoculation studies with KR083 and RRj228 revealed a high affinity to the Japonica rice variety such as Junambyeo than the Korean Tongil type variety such as Arumbyeo. Both KR083 and RRj228 strains showed rhizoplane and/or endophytic colonization in Japonica and Tongil types rice when soaked with the bacterial suspension of 1.1 × 10 5 cfu ml -1 for six and twelve hours. However, the total bacterial cell numbers were higher in the roots of Japonica variety than in the Tongil type. In inoculation trials with Daesanbyeo rice variety, the seedlings inoculated with KR181 and RRj228 at the rate of 2.0 × 10 6 cfu ml -1 showed yield increment of 35% and 33% (p < 0.01), respectively, so that they contributed to the replacement of chemical fertilizer at half doses of N, P 2 O 5 , and K 2 O in pots. In Junambyeo rice seedlings, the strain RRj228, when inoculated with a cell suspension of 1.8 × 10 6 cfu ml -1, promoted 3.4% higher yield at 70% dose than at a full dose level of N 110 kg ha -1 in field. These results suggest that the rhizobacteria KR181 and RRj228 are prospective strains for enhancing rice performance.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation, Root Colonization and Evaluation of Some Plant Growth-promoting Rhizobacteria in Paddy Rice

Kang¹,

Park²,

Ko³

et al. 2017

Korean J. Soil. Sci. Fert.

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“…4b; note that the bacterium is very densely gold labeled compared to the intercellular bacteria in panel b, and also that the majority of the labeling is of extracellular material surrounding it (small arrow). dans in association with rice (46) and also for expression of dinitrogenase reductase by Klebsiella pneumoniae in association with maize (11). However, an additional factor causing the carbon limitation on rice-associated BNF in the present study could be that the plants analyzed were in the early stages of growth (20 DAI).…”

Section: Discussionmentioning

confidence: 99%

Endophytic Colonization of Rice by a Diazotrophic Strain of Serratia marcescens

et al. 2001

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Six closely related N 2 -fixing bacterial strains were isolated from surface-sterilized roots and stems of four different rice varieties. The strains were identified as Serratia marcescens by 16S rRNA gene analysis. One strain, IRBG500, chosen for further analysis showed acetylene reduction activity (ARA) only when inoculated into media containing low levels of fixed nitrogen (yeast extract). Diazotrophy of IRBG500 was confirmed by measurement of 15 N 2 incorporation and by sequence analysis of the PCR-amplified fragment of nifH. To examine its interaction with rice, strain IRBG500 was marked with gusA fused to a constitutive promoter, and the marked strain was inoculated onto rice seedlings under axenic conditions. At 3 days after inoculation, the roots showed blue staining, which was most intense at the points of lateral root emergence and at the root tip. At 6 days, the blue precipitate also appeared in the leaves and stems. More detailed studies using light and transmission electron microscopy combined with immunogold labeling confirmed that IRBG500 was endophytically established within roots, stems, and leaves. Large numbers of bacteria were observed within intercellular spaces, senescing root cortical cells, aerenchyma, and xylem vessels. They were not observed within intact host cells. Inoculation of IRBG500 resulted in a significant increase in root length and root dry weight but not in total N content of rice variety IR72. The inoculated plants showed ARA, but only when external carbon (e.g., malate, succinate, or sucrose) was added to the rooting medium.

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“…Plant growth promoting effects of bacterial inoculation have been demonstrated on the in-vitro (ScholzSeidel and Ruppel, 1992;Merbach et al, 1998), pot (Boddey andDöbereiner, 1988) and field experiment level (Höflich and Ruppel, 1994;Van Nieuwenhove et al, 2000). However, particularly in field experiments, effects are often unreliable and the exact beneficial mechanisms are generally poorly understood.…”

Section: Introductionmentioning

confidence: 95%

Bacterial inoculation of maize affects carbon allocation to roots and carbon turnover in the rhizosphere

Schulze

Pöschel

2004

Plant Soil

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To assess the influence of bacteria inoculation on carbon flow through maize plant and rhizosphere, 14 C allocation after 14 CO 2 application to shoots over a 5-day period was determined. Plants were grown on C-and N-free quartz sand in two-compartment pots, separating root and shoot space. While one treatment remained uninoculated, treatments two and three were inoculated with Pantoea agglomerans (D5/23) and Pseudomonas fluorescens (Ps I A12), respectively, five days after planting. Bacterial inoculation had profound impacts on carbon distribution within the system. Root/rhizosphere respiration was increased and more carbon was allocated to roots of plants being inoculated. After five days of 14 CO 2 application, more ethanol-soluble substances were found in roots of inoculated treatments and lower rhizodeposition indicated intensive C turnover in the rhizosphere. In both inoculated treatments the intensity of photosynthesis measured as net-CO 2 -assimilation rates were increased when compared to the uninoculated plants. However, high C turnover in the rhizosphere reduced shoot growth of D5/23 inoculated plants, with no effect on shoot growth of Ps I A12 inoculated plants. A separation of labeled compounds in roots and rhizodeposition revealed that neutral substances (sugars) constituted the largest fraction. The relative fractions of sugars, amino acids and organic acids in roots and rhizodeposition suggest that amino acid exudation was particularly stimulated by bacterial inoculation and that turnover of this substance group is high in the rhizosphere.

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Establishment of Azorhizobium caulinodans in the rhizosphere of wetland rice ( Oryza sativa L.)

Cited by 35 publications

References 9 publications

Isolation, Root Colonization and Evaluation of Some Plant Growth-promoting Rhizobacteria in Paddy Rice

Isolation, Root Colonization and Evaluation of Some Plant Growth-promoting Rhizobacteria in Paddy Rice

Endophytic Colonization of Rice by a Diazotrophic Strain of Serratia marcescens

Bacterial inoculation of maize affects carbon allocation to roots and carbon turnover in the rhizosphere

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