Establishment of beet molasses as the fermentation substrate for industrial vitamin B₁₂ production by Pseudomonas denitrificans

Abstract

BACKGROUND: Fermentation of the industrial vitamin B 12 by Pseudomonas denitrificans usually utilizes sucrose or maltose as the sole carbon source, which results in increased medium costs. In order to decrease the fermentation cost, it is crucial and essential to employ a low-cost and convenient raw material as an alternative medium substrate for industrial vitamin B 12 production. RESULTS:The results obtained in chemically defined medium showed that glutamate and sucrose were favorable for cell growth and vitamin B 12 biosynthesis of P. denitrificans. Due to containing a mass of ingredients such as sucrose, glutamate and betaine, beet molasses was consequently chosen as the main medium substrate for industrial P. denitrificans fermentation in a 120 000 L fermenter. Vitamin B 12 production reached 181.75 mg L -1 . CONCLUSIONS: Beet molasses is a by-product of the sugar industry and is thus very inexpensive, and it was proved that beet molasses was an efficient and economical medium substrate for industiral vitamin B 12 fermentation by P. denitrificans.

Interactive performances of betaine on the metabolic processes of Pseudomonas denitrificans

“…According to the above results, it could be concluded that betaine metabolism would accompanied by large generation of glycine. Using the synthetic medium, our previous study had been clearly illustrated that glycine in fermentation broth would impose a severe restriction on the cell growth of P. denitrificans [11]. Thus, the highly vast amounts of generated extracellular glycine attributed to the actual reason why the cell growth rate under 10 g/l betaine was dramatically lower than that in the case of betaine absence (as shown in Fig.…”

Reactive Extraction of Betaine from Sugarbeet Processing Byproducts

“… 6 , 17 , 18 Molasses, the major and most valuable byproduct of beet sugar refining, has been the principal source of natural betaine. 19 − 21 Vinasse, a byproduct of ethanol fermentation of molasses, is another important source of betaine.…”

Biosynthesis of vitamin B12 by Propionibacterium freudenreichii subsp. shermanii ATCC 13673 using liquid acid protein residue of soybean as culture medium

“…These substrates are available in huge amounts, have very low added values, but are nutrient-rich for microbial growth (carbohydrates, proteins, and minerals). [13][14][15][16] One residue of particular interest in this case is the liquid acid protein residue of soybean (LAPRS), which is obtained as an effluent during the soybean-isolated protein process (SIP). For instance, one single industrial facility for SIP production generates approximately 50,000 m 3 of LAPRS monthly.…”

“…Another interesting factor regarding the use of this microorganism is the possibility of producing vitamin B12 using alternative culture media composed of agroindustry residues. These substrates are available in huge amounts, have very low added values, but are nutrient‐rich for microbial growth (carbohydrates, proteins, and minerals) 13‐16 . One residue of particular interest in this case is the liquid acid protein residue of soybean (LAPRS), which is obtained as an effluent during the soybean‐isolated protein process (SIP).…”