Establishment of Bovine-Induced Pluripotent Stem Cells

Su, Yue; Wang, Ling; Fan, Zhiqiang; Liu, Ying; Zhu, Jiaqi; Kaback, Deborah; Oudiz, J.; Patrick, Tayler; Yee, Siu Pok; Tian, X. Cindy; Polejaeva, Irina A.; Tang, Yaning

doi:10.3390/ijms221910489

Cited by 23 publications

(16 citation statements)

References 73 publications

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“…Additionally, EPZ004777 increased blastocyst rate of porcine cell somatic cell nuclear transfer (SCNT) ( Tao et al, 2017 ). EPZ004777 in combination with a WNT inhibitor was insufficient to reprogram notoriously refractory bovine mesenchymal stem cells to iPSCs, but could induce morphological changes ( Su et al, 2021 ). Thus overwhelming evidence indicates that H3K79me is a barrier for pluripotency acquisition across cell types and species ( Figure 2 ).…”

Section: Introductionmentioning

confidence: 99%

Connecting the DOTs on Cell Identity

Wille

Sridharan

2022

Front. Cell Dev. Biol.

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DOT1-Like (DOT1L) is the sole methyltransferase of histone H3K79, a modification enriched mainly on the bodies of actively transcribing genes. DOT1L has been extensively studied in leukemia were some of the most frequent onco-fusion proteins contain portions of DOT1L associated factors that mislocalize H3K79 methylation and drive oncogenesis. However, the role of DOT1L in non-transformed, developmental contexts is less clear. Here we assess the known functional roles of DOT1L both in vitro cell culture and in vivo models of mammalian development. DOT1L is evicted during the 2-cell stage when cells are totipotent and massive epigenetic and transcriptional alterations occur. Embryonic stem cell lines that are derived from the blastocyst tolerate the loss of DOT1L, while the reduction of DOT1L protein levels or its catalytic activity greatly enhances somatic cell reprogramming to induced pluripotent stem cells. DOT1L knockout mice are embryonically lethal when organogenesis commences. We catalog the rapidly increasing studies of total and lineage specific knockout model systems that show that DOT1L is broadly required for differentiation. Reduced DOT1L activity is concomitant with increased developmental potential. Contrary to what would be expected of a modification that is associated with active transcription, loss of DOT1L activity results in more upregulated than downregulated genes. DOT1L also participates in various epigenetic networks that are both cell type and developmental stage specific. Taken together, the functions of DOT1L during development are pleiotropic and involve gene regulation at the locus specific and global levels.

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Section: Introductionmentioning

confidence: 99%

Connecting the DOTs on Cell Identity

Wille

Sridharan

2022

Front. Cell Dev. Biol.

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“…Although the process of establishing pluripotent stem cell lines from relevant species such as cow [75], pig [55,76], goat [77], horse [78], and sheep [66,79] has progressed, it is still not at the large-scale level that is needed for the CM production bioprocess. However, there are promising studies such as those by Amilon et al in which they generated functional skeletal myotubes from equine iPSCs [80,81].…”

Section: Mammalian Ipscsmentioning

confidence: 99%

Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives

et al. 2022

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Global food systems are under significant pressure to provide enough food, particularly protein-rich foods whose demand is on the rise in times of crisis and inflation, as presently existing due to post-COVID-19 pandemic effects and ongoing conflict in Ukraine and resulting in looming food insecurity, according to FAO. Cultivated meat (CM) and cultivated seafood (CS) are protein-rich alternatives for traditional meat and fish that are obtained via cellular agriculture (CA) i.e., tissue engineering for food applications. Stem and progenitor cells are the building blocks and starting point for any CA bioprocess. This review presents CA-relevant vertebrate cell types and procedures needed for their myogenic and adipogenic differentiation since muscle and fat tissue are the primary target tissues for CM/CS production. The review also describes existing challenges, such as a need for immortalized cell lines, or physical and biochemical parameters needed for enhanced meat/fat culture efficiency and ways to address them.

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“…Bovine adult fibroblasts were only produced when nanog, SV40largeT, and KDM4A were expressed in combination with the other reprogramming ingredients [ 102 ]. By utilizing a specific component such as SV 40 large T, Lin28, and hTERT sheep somatic cells can be directly transformed to become induced pluripotent stem cell [ 103 , 104 ]. Another study reported the creation of live piglets using the iPSC technology in 2013 [ 13 ].…”

Section: Clean Meat Productionmentioning

confidence: 99%

iPSC Technology: An Innovative Tool for Developing Clean Meat, Livestock, and Frozen Ark

Verma¹,

Lee

Salamone

2022

Animals

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Induced pluripotent stem cell (iPSC) technology is an emerging technique to reprogram somatic cells into iPSCs that have revolutionary benefits in the fields of drug discovery, cellular therapy, and personalized medicine. However, these applications are just the tip of an iceberg. Recently, iPSC technology has been shown to be useful in not only conserving the endangered species, but also the revival of extinct species. With increasing consumer reliance on animal products, combined with an ever-growing population, there is a necessity to develop alternative approaches to conventional farming practices. One such approach involves the development of domestic farm animal iPSCs. This approach provides several benefits in the form of reduced animal death, pasture degradation, water consumption, and greenhouse gas emissions. Hence, it is essentially an environmentally-friendly alternative to conventional farming. Additionally, this approach ensures decreased zoonotic outbreaks and a constant food supply. Here, we discuss the iPSC technology in the form of a “Frozen Ark”, along with its potential impact on spreading awareness of factory farming, foodborne disease, and the ecological footprint of the meat industry.

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Establishment of Bovine-Induced Pluripotent Stem Cells

Cited by 23 publications

References 73 publications

Connecting the DOTs on Cell Identity

Connecting the DOTs on Cell Identity

Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives

iPSC Technology: An Innovative Tool for Developing Clean Meat, Livestock, and Frozen Ark

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