Establishment of caudal fin cell lines from tropical ornamental fishes Puntius fasciatus and Pristolepis fasciata endemic to the Western Ghats of India

Swaminathan, T. Raja; Basheer, V. S.; Gopalakrishnan, A; Rathore, Gaurav; Chaudhary, Dharmendra Kumar; Kumar, Raj; Jena, J. K.

doi:10.1016/j.actatropica.2013.07.020

Cited by 23 publications

(20 citation statements)

References 25 publications

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“…The HBF cell line has been established using the explant technique instead of enzymatic digestion methods as it has many advantages such as speed, ease, lesser damage to the cell surface and maintenance of cell interactions when compared to cell suspensions (Avella et al 1994). The explant culture technique was found to be the suitable method for developing primary cell cultures from H. brachysoma as observed in previous works on development of cell lines from L. calcarifer, Cyprinus carpio, Etroplus suratensis, Puntius denisonii and Puntius fasciatus, (Lakra et al 2010a, b;Swaminathan et al 2010Swaminathan et al , 2012Swaminathan et al , 2013Chaudhary et al 2013). The HBF cell line maintained stable growth for more than 62 passages, over a period of 2 years.…”

Section: Discussionmentioning

confidence: 80%

A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864)

et al. 2014

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A new epithelial cell line, Horabagrus brachysoma fin (HBF), was established from the caudal fin tissue of yellow catfish, H. brachysoma and characterized. This HBF cell line was maintained in Leibovitz's-15 medium supplemented with 15 % fetal bovine serum (FBS) and subcultured more than 62 times over a period of 20 months. The HBF cell line consists predominantly of epithelial cells and is able to grow at temperatures between 20 and 35°C with an optimum temperature of 28°C. The growth rate of these cells increased as the proportion of FBS increased from 5 to 20 % at 28°C with optimum growth at the concentrations of 15 % FBS. Partial amplification and sequencing of fragments of two mitochondrial genes 16S rRNA and COI confirmed that HBF cell line originated from yellow catfish. The HBF cells showed strong positive reaction to the cytokeratin marker, indicating that it was epithelial in nature. HBF cell line was inoculated with tissue homogenate from juveniles of Sea bass, Lates calcarifer infected with viral nervous necrosis virus (VNNV) and found not susceptible to VNNV. The extracellular products of Vibrio cholerae MTCC 3904 were toxic to the HBF cells. These cells were confirmed for the absence of Mycoplasma sp by PCR.

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Section: Discussionmentioning

confidence: 80%

A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864)

et al. 2014

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“…Cells died within a few hours when the culture temperature was shifted to 45°C (data not shown). A number of cell lines developed from freshwater fishes are known to grow best at 28-30°C (Tong et al 1997;Luc Rougee et al 2007;Ahmed et al 2009;Ku et al 2009;Swaminathan et al 2013). Ossum et al (2004) also reported that cells from warm water fishes can grow at 15-37°C incubation temperature.…”

Section: Discussionmentioning

confidence: 93%

“…The cells showed good growth in L-15 culture medium containing 15% FBS at 28°C. L-15 has been used as a common cell culture medium for growth of cells derived from various fish (Qin et al 2006;Lakra et al 2010;Swaminathan et al 2013). The growth of CCKF cells was influenced by the concentration of FBS, and we chose 15% FBS as optimum for CCKF culture.…”

Section: Discussionmentioning

confidence: 99%

“…CyHV-3 has become a globally distributed pathogen and affecting koi and common carps from at least 26 different countries of the world (Michel et al 2010;Maya et al 2011). Incidence of KHV disease has so far not been reported from India (Rathore et al 2012;Swaminathan et al 2013), in spite of KHV disease outbreaks happening in neighbouring Southeast Asian countries (Lio-Po et al 2009). However, unregulated and illegal international trade of ornamental fish, particularly koi carp can lead to introduction of KHV into India.…”

Section: Introductionmentioning

confidence: 95%

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Establishment and characterization of fin-derived cell line from ornamental carp, Cyprinus carpio koi, for virus isolation in India

Swaminathan

Basheer

Kumar

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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Cyprinus carpio koi fin (CCKF) cell line was established and characterized from the caudal fin tissue of ornamental common carp, C. carpio koi. This cell line has been maintained in L-15 medium supplemented with 15% foetal bovine serum (FBS) and subcultured more than 52 times over a period of 24 mo. The CCKF cell line consisted of epithelial cells and was able to grow at temperatures between 22 and 35°C with an optimum temperature of 28°C. The growth rate of these cells increased as the proportion of FBS increased from 2 to 20% with optimum growth at the concentrations of 15% FBS. Karyotype analysis revealed that the modal chromosome number of CCKF cells was 2n = 100. Partial amplification and sequencing of fragments of two mitochondrial genes 16S rRNA and COI confirmed that CCKF cell line originated from ornamental common carp. The CCKF cells showed strong reaction to the cytokeratin marker, indicating that it was epithelial in nature. The extracellular products of Vibrio cholerae MTCC 3904 and Aeromonas hydrophila were toxic to the CCKF cells and not susceptible to viral nervous necrosis virus (VNNV). These CCKF cells were confirmed for the absence of Mycoplasma sp. by polymerase chain reaction. Furthermore, 90% of viable cells could be effectively revived 4 mo after cryopreservation from CCKF cell population suggesting the possibility of long-term storage of the cells.

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“…They have been cultured in China for several 100 years and are still the most commonly kept fish in home aquariums. In recent years, they have been known to play an important role in the international fish trade (Swaminathan et al 2013); altogether, approximately 1800 strains of goldfish are traded (Silas et al 2011). With the development of goldfish aquaculture, several viruses in goldfish have been reported (Becker et al 2014;Stephens et al 2004;Xu et al 2013), including goldfish virus type 1 (GFV 1) (Berry et al 1983), goldfish virus type 2 (GFV 2) (Shea 1983;Shea and Berry 1984), herpes-type viruses (Jung and Miyazaki 1995), and it is likely that more exist.…”

Section: Introductionmentioning

confidence: 99%

Establishment from the snout and kidney of goldfish, Carassius auratus, of two new cell lines and their susceptibility to infectious pancreatic necrosis virus

Jing

Gao

Zhang

et al. 2015

Fish Physiol Biochem

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Goldfish Carassius auratus are commonly used in scientific research and have a significant economic value in the pet trade. In this study, two cell lines were established from the snout and kidney tissue of goldfish, in order to create a biological monitoring tool for viral diseases. Cell lines were optimally maintained at 25 °C in M199 medium supplemented with 15-20 % fetal bovine serum. Chromosomal analysis indicated that both cell lines remained diploid, with a mean chromosomal count of 100. Results of viral inoculation assays revealed that both cell lines shared similar patterns of viral susceptibility and production to infectious pancreatic necrosis virus (IPNV). The viral titers of IPNV in goldfish snout cell line (GFSe) and goldfish kidney cell line (GFKf) reached 10(6.8) and 10(5.9) TCID50/0.1 mL, respectively, within 7 days. The cytopathic effect could be observed when Cyprinid herpesvirus 2 was inoculated into these cells; however, the CPE disappeared after four passages. No CPE was observed in the cells infected by spring viremia carp virus or grass carp reovirus. These newly established cell lines will be a useful diagnostic tool for viral diseases in this fish species and also for the isolation and study of goldfish viruses in future.

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Establishment of caudal fin cell lines from tropical ornamental fishes Puntius fasciatus and Pristolepis fasciata endemic to the Western Ghats of India

Cited by 23 publications

References 25 publications

A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864)

A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864)

Establishment and characterization of fin-derived cell line from ornamental carp, Cyprinus carpio koi, for virus isolation in India

Establishment from the snout and kidney of goldfish, Carassius auratus, of two new cell lines and their susceptibility to infectious pancreatic necrosis virus

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