Establishment of Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA)-positive nasopharyngeal carcinoma hybrid cell line (NPC-KT)

Abstract

Primary nasopharyngeal carcinoma (NPC) cells were fused to hypoxanthine-guanine phosphoribosyltransferase (HGPRT)-defective cells derived from adenoid tissues using Sendai virus. Some of the fused cells developed into epithelial-like hybrid cells in a selective HAT medium. The hybrid cells (NPC-KT) were Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA)-positive cells. There have been no reports on the establishment of EBNA-positive epithelial cell lines derived from NPC. Thus, the epithelial-like hybrid cells might serve as an in vitro model for studying the biologic activity of NPC-associated EBV.

A Positive Autoregulatory Loop of LMP1 Expression and STAT Activation in Epithelial Cells Latently Infected with Epstein-Barr Virus

“…C-666-1, an EBV-positive NPC cell line (9), was maintained in RPMI 1640 plus 10% fetal bovine serum. NPC-KT, an EBV-positive epithelial cell line (74), was cultured in Dulbecco's modified Eagle's medium plus 10% fetal bovine serum. Daudi and B95-8 (EBV positive) and CA46 (EBV negative) B-cell lines were cultured in RPMI 1640 plus 10% fetal bovine serum.…”

Use of Adenovirus Vectors Expressing Epstein-Barr Virus (EBV) Immediate-Early Protein BZLF1 or BRLF1 To Treat EBV-Positive Tumors

“…NPC-KT is an EBV genome-positive NPC epithelial hybrid cell line, which was established by fusion of primary EBV genome-positive NPC epithelial cells with an epithelial cell line derived from human adenoid tissue (50). NPC-KT cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and penicillin-streptomycin.…”

The major immunogenic epitopes of Epstein-Barr virus (EBV) nuclear antigen 1 are encoded by sequence domains which vary among nasopharyngeal carcinoma biopsies and EBV-associated cell lines.

“…NPC-KT was derived by fusion of primary cells from a Japanese NPC biopsy with GHPRT-AD-AH cells and selected with HAT medium. The epithelial-like, hybrid NPC-KT cells were EBNA-positive (Takimoto et al, 1984). DNA samples of NPC biopsies were collected from the Hospital of National Taiwan University.…”