Establishment of fibroblast and myofibroblast phenotypes for use in in vitro co-culture models

Ramklowan, D.S.H.; Snyman, Celia; Vyver, Marí van de; Niesler, Carola U.

doi:10.1016/j.biochi.2022.10.017

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…Initially, we explored reducing FBS concentration in the cell culture media from 10% to two or 0%. The effect of reducing FBS achieved to reduce cellular activation through the decrease of αSMA stress fibers, in agreement with other studies (Supplementary Figure S1; (Baranyi et al, 2019;Zhang et al, 2019;Pesce et al, 2023;Ramklowan et al, 2023)). However, as reported by other studies, this reduction compromises cellular viability (Rashid and Coombs, 2019;van Vijven et al, 2021).…”

Section: Discussionsupporting

confidence: 92%

GelMA hydrogel dual photo-crosslinking to dynamically modulate ECM stiffness

Smits,

van der Pol,

Goumans

et al. 2024

Front. Bioeng. Biotechnol.

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The dynamic nature of the extracellular matrix (ECM), particularly its stiffness, plays a pivotal role in cellular behavior, especially after myocardial infarction (MI), where cardiac fibroblasts (cFbs) are key in ECM remodeling. This study explores the effects of dynamic stiffness changes on cFb activation and ECM production, addressing a gap in understanding the dynamics of ECM stiffness and their impact on cellular behavior. Utilizing gelatin methacrylate (GelMA) hydrogels, we developed a model to dynamically alter the stiffness of cFb environment through a two-step photocrosslinking process. By inducing a quiescent state in cFbs with a TGF-β inhibitor, we ensured the direct observation of cFbs-responses to the engineered mechanical environment. Our findings demonstrate that the mechanical history of substrates significantly influences cFb activation and ECM-related gene expression. Cells that were initially cultured for 24 h on the soft substrate remained more quiescent when the hydrogel was stiffened compared to cells cultured directly to a stiff static substrate. This underscores the importance of past mechanical history in cellular behavior. The present study offers new insights into the role of ECM stiffness changes in regulating cellular behavior, with significant implications for understanding tissue remodeling processes, such as in post-MI scenarios.

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Section: Discussionsupporting

confidence: 92%

GelMA hydrogel dual photo-crosslinking to dynamically modulate ECM stiffness

Smits,

van der Pol,

Goumans

et al. 2024

Front. Bioeng. Biotechnol.

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show abstract

Section: Discussionsupporting

confidence: 92%

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“…The activation of fibroblasts to myofibroblasts is a critical step during the wound healing process. Compared with fibroblasts, myofibroblasts are characterized by the expression of high levels of α-SMA that form contractile bundles and generate large cellular tractions to support wound closure. , It has been reported that the myofibroblast transition is promoted by soluble factors such as TGF-β and by mechanical forces as well. , Taking the NIR light and thermal responsive properties of PIC/CM@NPs hydrogel composites into consideration, we characterized the temperature and stiffness changes after NIR irradiation. CM@NPs slightly exhibit a photothermal effect that partially converts light into heat, leading to an approximate 4 °C increases from 37 to 41 °C under NIR for 150 s (Figure S7).…”

mentioning

confidence: 99%

“…Compared with fibroblasts, myofibroblasts are characterized by the expression of high levels of α-SMA that form contractile bundles and generate large cellular tractions to support wound closure. 36,37 It has been reported that the myofibroblast transition is promoted by soluble factors such as TGF-β and by mechanical forces as well. 36,38 Taking the NIR light and thermal responsive properties of PIC/CM@NPs hydrogel composites into consideration, we characterized the temperature and stiffness changes after NIR irradiation.…”

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confidence: 99%

Microenvironment with NIR-Controlled ROS and Mechanical Tensions for Manipulating Cell Activities in Wound Healing

Sun,

Jin,

Bao

et al. 2024

Nano Lett.

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The extracellular matrix (ECM) orchestrates cell behavior and tissue regeneration by modulating biochemical and mechanical signals. Manipulating cell–material interactions is crucial for leveraging biomaterials to regulate cell functions. Yet, integrating multiple cues in a single material remains a challenge. Here, near-infrared (NIR)-controlled multifunctional hydrogel platforms, named PIC/CM@NPs, are introduced to dictate fibroblast behavior during wound healing by tuning the matrix oxidative stress and mechanical tensions. PIC/CM@NPs are prepared through cell adhesion-medicated assembly of collagen-like polyisocyanide (PIC) polymers and cell-membrane-coated conjugated polymer nanoparticles (CM@NPs), which closely mimic the fibrous structure and nonlinear mechanics of ECM. Upon NIR stimulation, PIC/CM@NPs composites enhance fibroblast cell proliferation, migration, cytokine production, and myofibroblast activation, crucial for wound closure. Moreover, they exhibit effective and toxin removal antibacterial properties, reducing inflammation. This multifunctional approach accelerates healing by 95%, highlighting the importance of integrating biochemical and biophysical cues in the biomaterial design for advanced tissue regeneration.

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Establishment of fibroblast and myofibroblast phenotypes for use in in vitro co-culture models

Cited by 6 publications

References 29 publications

GelMA hydrogel dual photo-crosslinking to dynamically modulate ECM stiffness

GelMA hydrogel dual photo-crosslinking to dynamically modulate ECM stiffness

Table1.DOCX

Microenvironment with NIR-Controlled ROS and Mechanical Tensions for Manipulating Cell Activities in Wound Healing

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