Establishment of functional human pituitary tumor cell cultures

Abstract

Five primary human pituitary tumor cell cultures were initiated from adenoma fragments obtained from patients with prolactin-secreting adenomas and acromegaly. Functional cell cultures were maintained and propagated in monolayer or suspension culture for up to 9 months. Optimal cell viability and growth were achieved using Ham's F10 medium enriched with 20% fetal bovine serum, although cells from a patient with acromegaly also grew in serum-free, defined, hormone-containing medium. Bromocriptine (100 ng/ml) did not alter the growth curve of replicating cells derived from a patient with acromegaly. These cells initially secreted 5.5 micrograms human growth hormone/10(6) cells, and hormone production diminished after 6 wk. Prolactin secretion by cells derived from prolactinomas (0.5 to 1.3 micrograms/10(6) cells/24 h) was stimulated by thyrotropin-releasing hormone (10 ng/ml) in two of the cultures. Both dopamine (10 ng/ml) and nickel chloride (1 mM) suppressed PRL secretion. These studies demonstrate that responsive human pituitary tumor cell cultures can be initiated and maintained.

Three Dimensional Models of Endocrine Organs and Target Tissues Regulated by the Endocrine System

“…Four immortalized cell lines originating from human pituitary adenomas exist to date: HPA [ 49 ], GX [ 50 ], HP75 [ 51 ] and PDFS [ 52 ]. Human primary cultures can be maintained as 2D cultures in vitro for a few passages and are mostly used to decipher the mechanisms of hormone secretion [ 53 , 54 , 55 , 56 ]. Although PitNET organoid literature is sparse, a few publications have emerged in the last few years.…”

Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells

“…However, this culture of secretory human pituitary cell tumors is not an efficient development; dispersed tumor pituitary cells grow poorly in culture, the cells exhibit limited attachment and spreading, and the cells typically form floating aggregates in the culture dish 9,10 . Several attempts have been made to obtain a tumor pituitary cell suspension, including an enzymatic and mechanical dispersion approach 11 , a solely mechanical dispersion approach 12 and the culturing of tumor explants 10 . With these approaches, different authors have obtained viable adherent cultures for different periods of time.…”

In vitro διεύρυνση της εκκριτικής δραστηριότητας των αδενωμάτων υπόφυσης

“…Έχει επίσης εφαρμοστεί όπως και ο προηγούμενος τύπος σε εμβρυολογικές μελέτες της ανθρώπινης υπόφυσης (Siler-Khodr et al, 1974, Goodyeretal, 1977). Γ) Η καλλιέργεια διάσπαρτων κυττάρων (dispersed cell culture) παρουσιάζει περισσότερα πλεονεκτήματα γιατί παρέχει ισότιμες συνθήκες ζωής για όλα τα καλλιεργούμενα κύτταρα και επιτρέπει την ακριβή μέτρη ση της ζωτικότητας και του πολλαπλασιασμού τους (Vale et al, 1972, Melmed et al, 1982. Οι διακυτταρικές σχέσεις, σε αυτόν τον τύπο κυτταροκαλλιέργειας χάνονται.…”