Establishment of Glucocorticoid-Mediated Transcriptional Induction of the Rice XA21 Pattern Recognition Receptor

Park, Chang-Jin; Canlas, Patrick E.; Ronald, Pamela C.

doi:10.1007/s12374-011-9188-1

Cited by 13 publications

(16 citation statements)

References 34 publications

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“…However, due to the fact that in many cases non-pathogenic microbes might also possess such molecules, the strict term "pathogen-associated" is limiting and will be replaced in the following by the term MAMP. MAMPs are perceived by corresponding plant pattern recognition receptors (PRR) 23 at low concentrations [24][25][26][27] and display many diverse structures, such as carbohydrates, peptidoglycans, peptides, proteins, lipids, and sterols. [26][27][28][29] MAMPs can be constituents of the outer layers of fungal pathogens, such as chitin (1) fragments, 30,31 of oomycete (Phytophthora) pathogens in the case of b-(1,3)-(1,6)-glucan (2) fragments 32 and Pep-13 (3), a peptide of the cell wall transglutaminase of Phytophthora.…”

Section: Definition Of Elicitors Effectors and Modulatorsmentioning

confidence: 99%

Natural elicitors, effectors and modulators of plant responses

2012

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In plants, successful defense relies on fast and specific response to biotic attack. In plant-microbial and plant-plant interactions several elicitors, effectors and modulators are recognized by specific and unspecific receptors that trigger signal cascades eventually leading to gene expression and plant responses. Here we review the chemical nature and signaling pathways of natural products released by microorganisms, herbivores, and plants during pathogenic infections, herbivory, symbioses and allelopathic interactions.

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Section: Definition Of Elicitors Effectors and Modulatorsmentioning

confidence: 99%

Natural elicitors, effectors and modulators of plant responses

2012

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“…The growth perturbations were caused probably by the GVG activator that was binding to cis-regulatory elements in the plant genome with sequence homology to GAL4 upon Dex activation (Kang et al, 1999). Nevertheless, the system was used successfully in a number of studies in rice (Chern et al, 2013;Nakayama et al, 2013;Onodera et al, 2018, Park et al, 2012Qu et al, 2009) in which the negative effects were reduced by shortening the time of Dex exposure or selecting lines with low GVG expression levels and mild phenotype that could be used as activator lines.…”

Section: Discussionmentioning

confidence: 99%

The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does NOT cause plant growth defects in rice (Oryza sativa)

Samalova

Moore

2021

Preprint

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SummaryInducible systems for transgene expression activated by a chemical inducer or an inducer of non-plant origin are desirable tools for both basic plant research and biotechnology. Although, the technology has been widely exploited in model plants, it has not been optimised for use with the major monocotyledonous crop species, namely rice. We have adapted the dexamethasone-inducible pOp6/LhGR system for rice and shown that it is fast, sensitive and tightly regulated, with high levels of induction that remain stable over several generations. Most importantly, we have shown that the system does not cause negative growth defects in vitro or in soil grown plants. Interestingly in the process of testing, we found that another steroid, triamcinolone acetonide, is a more potent inducer in rice than dexamethasone. We present serious considerations for the construct design to avoid undesirable effects caused by the system in plants, leakiness and possible silencing, as well as simple steps how to maximize translation efficiency of a gene of interest. Finally, we compare the performance of the pOp6/LhGR system with other chemically inducible systems tested in rice in terms of the properties of an ideal inducible system.Significance statementThe non-monocot codon-optimized version of the dexamethasone inducible pOp6/LhGR system does not cause severe developmental perturbations in rice plants.

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“…In our system, about half of the independently transformed rice lines displayed weak or moderate leaky expression (Park et al , 2012). Therefore, it is critical to identify the transgenic lines that display the least leaky expression while maintaining highly inducible expression.…”

Section: Notesmentioning

confidence: 97%

“…To confirm non-leaky expression of Xa21 , selected lines should be fully susceptible to Xoo in the absence of DEX and resistant to Xoo after DEX is applied. Additionally, Xa21 gene expression and protein abundance should be low or not detectable by quantitative RT-PCR and western blot analysis before DEX treatment and should be induced by DEX application (Park et al , 2012). …”

Section: Notesmentioning

confidence: 99%

The Use of a Dexamethasone-inducible System to Synchronize Xa21 Expression to Study Rice Immunity

Caddell¹,

Wei²,

Park³

et al. 2015

BIO-PROTOCOL

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Inducible gene expression systems offer researchers the opportunity to synchronize target gene expression at particular developmental stages and in particular tissues. The glucocorticoid receptor (GR), a vertebrate steroid receptor, has been well adopted for this purpose in plants. To generate steroid-inducible plants, a construct of GAL4-binding domain-VP16 activation domain-GR fusion (GVG) with the target gene under the control of upstream activation sequence (UAS) has been developed and extensively used in plant research. Immune receptors perceive conserved molecular patterns secreted by pathogens and initiate robust immune responses. The rice immune receptor, XA21, recognizes a molecular pattern highly conserved in all sequenced genomes of Xanthomonas, and confers robust resistance to X. oryzae pv. oryzae (Xoo). However, identifying genes downstream of XA21 has been hindered because of the restrained lesion and thus limited defense response region in the plants expressing Xa21. Inducible expression allows for a synchronized immune response across a large amount of rice tissue, well suited for studying XA21-mediated immunity by genome-wide approaches such as transcriptomics and proteomics. In this protocol, we describe the use of this GVG system to synchronize Xa21 expression.

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Establishment of Glucocorticoid-Mediated Transcriptional Induction of the Rice XA21 Pattern Recognition Receptor

Cited by 13 publications

References 34 publications

Natural elicitors, effectors and modulators of plant responses

Natural elicitors, effectors and modulators of plant responses

The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does NOT cause plant growth defects in rice (Oryza sativa)

The Use of a Dexamethasone-inducible System to Synchronize Xa21 Expression to Study Rice Immunity

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