Establishment of hairy root cultures of Salvia bulleyana Diels for production of polyphenolic compounds

Wojciechowska, Marta; Owczarek, Aleksandra; Kiss, Anna K.; Grąbkowska, Renata; Olszewska, Monika A.; Grzegorczyk-Karolak, Izabela

doi:10.1016/j.jbiotec.2020.05.002

Cited by 37 publications

(38 citation statements)

References 52 publications

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“…The chromatographic behavior and fragmentation pattern of peak 10 was in agreement with the literature data for yunnaneic acid E [12]. Peak 21 was identified as salvianolic acid K, as described previously for S. bulleyana [13], and peak 16 as its isomer, found previously in S. cadmica Boiss. [20].…”

Section: Identification Of Phenolic Acid Derivativessupporting

confidence: 89%

“…This is the first report of the presence of phenylethanoids in the organs of S. bulleyana. The compound, with data consistent with that of compound 38, had been detected earlier in hydromethanolic extract from S. bulleyana transformed roots, but in that paper it was not identified [13]. Phenylethanoids are not common metabolites of the Salvia genus, but they have been detected in the shoots of some species, for example of S. viridis [7].…”

Section: Identification Of Phenolic Acid Derivativessupporting

confidence: 60%

“…The identified compounds can be classified into two structural groups: phenolic acids and their derivatives (28 compounds) and flavonoids (nine compounds); however, flavonoids were detected only in the shoot extract. The compounds were identified by comparing the obtained spectra data with those of the authentic standards, and/or those available in the literature [7][8][9][10][11][12][13].…”

Section: Quantitative Analysis Of Analyzed Hydromethanolic Extractsmentioning

confidence: 99%

“…UV spectra were recorded in the range of 200-400 nm, and chromatograms were acquired at 325 nm. The compounds were analyzed in negative ion mode, and identified by comparing the data on their retention time, UV-vis, and mass spectra with the data obtained for standards, or the data reported in the literature [7][8][9][10][11][12][13].…”

Section: Qualitative Uhplc-pda-esi-ms Analysismentioning

confidence: 99%

“…Before the analysis, extracts were dissolved in 80% methanol and filtered through a PTFE syringe filter (25 mm, 0.22 µm). Compounds were analyzed using an Elite LaChrom Hitachi system according to the method described by Wojciechowska et al [13]. The tentatively identified peaks were quantified relatively, using calibration curves of closely related authentic standards: rosmarinic acid for quantification of rosmarinic acid hexoside and methylrosmarinate; salvianolic acid B and salvianolic acid F for quantification of salvianolic acid B isomer and salvianolic F isomer, respectively; salvianolic acid A for quantification of salvianolic acid K and lithospermic acid isomer.…”

Section: Quantitative Hplc-pda Analysis Of Polyphenolic Acidsmentioning

confidence: 99%

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Phytochemical Profile and Antioxidant Activity of Aerial and Underground Parts of Salvia bulleyana Diels. Plants

et al. 2020

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Plants have been used for medical purposes since ancient times. However, a detailed analysis of their biological properties and their associated active compounds is needed to justify their therapeutic use in modern medicine. The aim of the study was to identify and quantify the phenolics present in hydromethanolic extracts of the roots and shoots of the Chinese Salvia species, Salvia bulleyana. The qualitative and quantitative analyses were carried out by ultrahigh-performance liquid chromatography with electrospray ionization mass spectrometry detection (UHPLC-PDA-ESI-MS), and high-performance liquid chromatography with photodiode array (HPLC-PDA) detection. The extracts of S. bulleyana were also screened for their antioxidant activity using ferric ion (Fe3+) reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) cation (ABTS), superoxide radical anion (O2•–), and inhibition of lipid peroxidation assays. The S. bulleyana extracts were found to contain 38 substances, of which 36 were phenols, with a total level of 14.4 mg/g DW (dry weight) in shoots, and 23.1 mg/g DW in roots. Twenty-eight phenols were polyphenolic acids or their derivatives, the most abundant in shoots being rosmarinic acid, and in roots, salvianolic acid K followed by rosmarinic acid. The other major phenolic acids were caffeic acid, caffeoyl-threonic acids, isomers of lithospermic acid, salvianolic acid F, salvianolic acid B, and yunnaneic acid E. In addition to polyphenolic acids, nine flavonoids were detected in the shoot extract. While both extracts showed significant antioxidant activity, the shoot extract, containing both polyphenolic acids and flavonoids, demonstrated a slightly greater antioxidant potential in some of the anti-radical tests than the roots. However, the root extract proved to be slightly more effective in the lipid peroxidation inhibition test. Thus, S. bulleyana was demonstrated as a promising source of antioxidants, and worthy of further more detailed studies.

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Section: Identification Of Phenolic Acid Derivativessupporting

confidence: 89%

Section: Identification Of Phenolic Acid Derivativessupporting

confidence: 60%