Establishment of Hertwig’s Epithelial Root Sheath/Epithelial Rests of Malassez Cell Line from Human Periodontium

Nam, Hyunwoo; Kim, Jihye; Kim, Jae-Won; Park, Joo-Cheol; Kim, Jung-Wook; Lee, Gene

doi:10.14348/molcells.2014.0161

Cited by 24 publications

(27 citation statements)

References 21 publications

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“…All experiments were conducted at passage 6. Human Hertwig’s Epithelial Rest Sheath/Epithelial rest of Malassez (HERS/ERM) cells were primarily isolated and cultured according to previous report (Nam et al, 2014). …”

Section: Methodsmentioning

confidence: 99%

In Vivo Angiogenic Capacity of Stem Cells from Human Exfoliated Deciduous Teeth with Human Umbilical Vein Endothelial Cells

Kim¹,

Kim²,

Kim³

et al. 2016

Molecules and Cells

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Dental pulp is a highly vascularized tissue requiring adequate blood supply for successful regeneration. In this study, we investigated the functional role of stem cells from human exfoliated deciduous teeth (SHEDs) as a perivascular source for in vivo formation of vessel-like structures. Primarily isolated SHEDs showed mesenchymal stem cell (MSC)-like characteristics including the expression of surface antigens and in vitro osteogenic and adipogenic differentiation potentials. Moreover, SHEDs were positive for NG2, α-smooth muscle actin (SMA), platelet-derived growth factor receptor beta (PDGFRβ), and CD146 as pericyte markers. To prove feasibility of SHEDs as perivascular source, SHEDs were transplanted into immunodeficient mouse using Matrigel with or without human umbilical vein endothelial cells (HUVECs). Transplantation of SHEDs alone or HUVECs alone resulted in no formation of vessel-like structures with enough red blood cells. However, when SHEDs and HUVECs were transplanted together, extensive vessel-like structures were formed. The presence of murine erythrocytes within lumens suggested the formation of anastomoses between newly formed vessel-like structures in Matrigel plug and the host circulatory system. To understand underlying mechanisms of in vivo angiogenesis, the expression of angiogenic cytokine and chemokine, their receptors, and MMPs was compared between SHEDs and HUVECs. SHEDs showed higher expression of VEGF, SDF-1α, and PDGFRβ than HUVECs. On the contrary, HUVECs showed higher expression of VEGF receptors, CXCR4, and PDGF-BB than SHEDs. This differential expression pattern suggested reciprocal interactions between SHEDs and HUVECs and their involvement during in vivo angiogenesis. In conclusion, SHEDs could be a feasible source of perivascular cells for in vivo angiogenesis.

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Section: Methodsmentioning

confidence: 99%

In Vivo Angiogenic Capacity of Stem Cells from Human Exfoliated Deciduous Teeth with Human Umbilical Vein Endothelial Cells

Kim¹,

Kim²,

Kim³

et al. 2016

Molecules and Cells

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“…Ex vivo-expanded ERM exhibited both bone marrow mesenchymal stem/progenitor cell-(heat shock protein-90b, CD44 and CD29) and epithelial cell-markers (epithelial membrane protein-1, cytokeratin-8, and E-cadherin) proving their stem/progenitor cell-like properties [66]. An ERM cell line was further successfully generated from human periodontium to be used for future research [67]. ERM cells when cocultured with dental pulp cells were differentiated into ameloblast-like cells and produced enamel-like tissues [68].…”

Section: Enamel Regenerationmentioning

confidence: 99%

Tissue Engineering Approaches for Enamel, Dentin, and Pulp Regeneration: An Update

Ahmed

Abouauf

AbuBakr

et al. 2020

Stem Cells International

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Stem/progenitor cells are undifferentiated cells characterized by their exclusive ability for self-renewal and multilineage differentiation potential. In recent years, researchers and investigations explored the prospect of employing stem/progenitor cell therapy in regenerative medicine, especially stem/progenitor cells originating from the oral tissues. In this context, the regeneration of the lost dental tissues including enamel, dentin, and the dental pulp are pivotal targets for stem/progenitor cell therapy. The present review elaborates on the different sources of stem/progenitor cells and their potential clinical applications to regenerate enamel, dentin, and the dental pulpal tissues.

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“…Although current studies use murine, rat and porcine tooth germ tissues, animal-derived cells are not suitable for clinical applications in humans. Epithelial Rests of Malassez (REM), present in mature human periodontal tissues, may be a suitable source for human dental epithelium [130]. In addition, current attempts to reprogram cell sources include the development of spontaneously derived immortalized murine dental epithelial cell lines, which when grown in low calcium-supplemented media, can express enamel markers such as amelogenin [131,132], and the use of single-cell suspensions generated from tooth germ tissue [133].…”

Section: Unmet Needsmentioning

confidence: 99%

Whole-organ engineering with natural extracellular materials

Sanaan¹,

Walboomers²,

Yelick³

2016

Nanomaterials and Regenerative Medicine

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Establishment of Hertwig’s Epithelial Root Sheath/Epithelial Rests of Malassez Cell Line from Human Periodontium

Cited by 24 publications

References 21 publications

In Vivo Angiogenic Capacity of Stem Cells from Human Exfoliated Deciduous Teeth with Human Umbilical Vein Endothelial Cells

In Vivo Angiogenic Capacity of Stem Cells from Human Exfoliated Deciduous Teeth with Human Umbilical Vein Endothelial Cells

Tissue Engineering Approaches for Enamel, Dentin, and Pulp Regeneration: An Update

Whole-organ engineering with natural extracellular materials

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