Establishment of High and Low Metastasis Cell Lines Derived from a Human Tongue Squamous Cell Carcinoma

Nakayama, Shuko; Sasaki, Akira; Mese, Hiroshi; Alcalde, Rafael; Matsumura, Tomohiro

doi:10.1159/000024515

Cited by 44 publications

(50 citation statements)

References 18 publications

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“…17 Decreased levels of E-cadherin have also been associated with a metastatic phenotype in SCCHN cell lines. 18 This relationship has also been shown in tumor specimens, 19 indicating that loss of E-cadherin may be an important step in the development of metastasis in SCCHN.…”

Section: Fig 3 (A)mentioning

confidence: 62%

Change of E-Cadherin by Hepatocyte Growth Factor and Effects on the Prognosis of Hypopharyngeal Carcinoma

Kim

Kahng

et al. 2007

Ann Surg Oncol

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Background: Hepatocyte growth factor (HGF) is known to induce scattering in various epithelial cells, and E-cadherin plays important roles in the maintenance of cell-cell adhesion. However, the mechanisms surrounding these actions are not fully understood. Therefore, we examined how HGF affects the expression and distribution of E-cadherin. In addition, we observed the relationship between prognosis and modulation of E-cadherin by HGF in hypopharyngeal carcinoma.Methods: Tumor tissues from 66 patients with hypopharyngeal squamous cell carcinoma were evaluated for the expression of HGF, its receptor (c-Met), and E-cadherin. Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot test were performed on hypopharyngeal cancer tissues. The association and changes of E-cadherin with HGF treatment in a hypopharyngeal cancer cell line were investigated by RT-PCR, Western blot analysis, inhibition assay, immunofluorescence staining, and invasion assay.Results: E-cadherin expression was found in 87.9% of squamous cell carcinomas; these could be further classified as membranous type (46.9%) or nonmembranous type (53.1%). The expression of HGF in tumors with nonmembranous type E-cadherin expression was far higher than in tumors with membranous expression. Nonmembranous type E-cadherin expression correlated significantly with lymph node metastasis, distant metastasis, and recurrence (P < .05). HGF decreased the expression of E-cadherin and induced the translocation of Ecadherin to the cytoplasm. HGF and E-cadherin neutralizing antibody stimulated dispersion, and HGF significantly enhanced the invasion of hypopharyngeal cancer cells in a dosedependent manner (P < .05).Conclusions: These results suggest that HGF can modulate the expression and intracellular localization of E-cadherin in hypopharyngeal cancer cells. In addition, these results indicate that changes in E-cadherin by HGF can affect the prognosis of hypopharyngeal carcinoma.

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Section: Fig 3 (A)mentioning

confidence: 62%

Change of E-Cadherin by Hepatocyte Growth Factor and Effects on the Prognosis of Hypopharyngeal Carcinoma

Kim

Kahng

et al. 2007

Ann Surg Oncol

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“…In this research, we used the UM1 and UM2 cells. These cells are paired cell lines from a single lingual squamous cell carcinoma patient with different metastatic potentials (13). Specifically, UM1 is more aggressive than UM2 in terms of cell invasion (14).…”

Section: Discussionmentioning

confidence: 99%

“…The UM1 and UM2 cells used in this study are paired cell lines with different metastatic potential that were generated from a single patient with lingual squamous cell carcinoma (13). These cell lines were maintained in Dulbecco's modified Eagle's medium/F12 (DMEM/F12) supplemented with 10% FBS, 100 U/ml penicillin and 100 µg/ ml streptomycin.…”

Section: Methodsmentioning

confidence: 99%

Notch1 signaling pathway participates in cancer invasion by regulating MMPs in lingual squamous cell carcinoma

Wei²,

Qian³

et al. 2011

Oncol Rep

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Abstract. Some studies show the Notch signaling pathway may participate in carcinoma invasion and metastasis. However, the mechanisms by which Notch1 mediates cell invasion and migration, especially in lingual squamous cell carcinoma, are not yet known. In the current study, we demonstrated for the first time the anti-invasion and anti-metastasis effect of down-regulation of Notch1 in lingual squamous cell carcinoma. Down-regulation of Notch1 could be an effective approach for inhibition of the expression of matrix metalloproteinase (MMP)-2 and MMP-9 resulting in the inhibition of invasion and metastasis, which could be useful for devising novel preventive and therapeutic strategies for lingual squamous cell carcinoma.

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“…Human oral cancer cell line, UM1 [52] and the zinc-inducible OAZ transfectant (pMT/CB6 + HuFSAZ-wt) and the mock vector transfectant (UM1-pMT/CB6 + ) were cultured as previously described [25]. The expression of OAZ gene from the pMT/CB6 + HuFSAZ-wt was induced by 100 µM ZnSO 4 treatment and the protein samples were harvested after one week ZnSO 4 treatment.…”

Section: Methodsmentioning

confidence: 99%

Ornithine Decarboxylase Antizyme Induces Hypomethylation of Genome DNA and Histone H3 Lysine 9 Dimethylation (H3K9me2) in Human Oral Cancer Cell Line

et al. 2010

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BackgroundMethylation of CpG islands of genome DNA and lysine residues of histone H3 and H4 tails regulates gene transcription. Inhibition of polyamine synthesis by ornithine decarboxylase antizyme-1 (OAZ) in human oral cancer cell line resulted in accumulation of decarboxylated S-adenosylmethionine (dcSAM), which acts as a competitive inhibitor of methylation reactions. We anticipated that accumulation of dcSAM impaired methylation reactions and resulted in hypomethylation of genome DNA and histone tails.Methodology/Principal FindingsGlobal methylation state of genome DNA and lysine residues of histone H3 and H4 tails were assayed by Methylation by Isoschizomers (MIAMI) method and western blotting, respectively, in the presence or absence of OAZ expression. Ectopic expression of OAZ mediated hypomethylation of CpG islands of genome DNA and histone H3 lysine 9 dimethylation (H3K9me2). Protein level of DNA methyltransferase 3B (DNMT3B) and histone H3K9me specific methyltransferase G9a were down-regulated in OAZ transfectant.Conclusions/SignificanceOAZ induced hypomethylation of CpG islands of global genome DNA and H3K9me2 by down-regulating DNMT3B and G9a protein level. Hypomethylation of CpG islands of genome DNA and histone H3K9me2 is a potent mechanism of induction of the genes related to tumor suppression and DNA double strand break repair.

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Establishment of High and Low Metastasis Cell Lines Derived from a Human Tongue Squamous Cell Carcinoma

Cited by 44 publications

References 18 publications

Change of E-Cadherin by Hepatocyte Growth Factor and Effects on the Prognosis of Hypopharyngeal Carcinoma

Change of E-Cadherin by Hepatocyte Growth Factor and Effects on the Prognosis of Hypopharyngeal Carcinoma

Notch1 signaling pathway participates in cancer invasion by regulating MMPs in lingual squamous cell carcinoma

Ornithine Decarboxylase Antizyme Induces Hypomethylation of Genome DNA and Histone H3 Lysine 9 Dimethylation (H3K9me2) in Human Oral Cancer Cell Line

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