Establishment of human tubal epithelial cells for coculture in an IVF program

Walker, D. J.; Vlad, Marcela; Kennedy, C R

doi:10.1007/bf02765775

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…ICI 182,780 (10 -6 M) completely blocked the estrogen's effect, suggesting that the stimulation is mediated mainly through ERcL (Chae et al, 1998) and is estrogen-specific, as observed in primary cultures (Goldenberg and Friedman, 1995;Joshi, 1995;Walter, 1995;Abe and Hoshi, 1997;Walker et al, 1997). Expression of estrogen receptor 13 was examined by immunoblot analysis, but it was not detectable (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…Secretory epithelial cells show functional changes in secretion during the estrous cycle; the activity is greatest during proestrus and estrus (Buhi et al, 1989). Estrogen is shown to promote secretion in primary culture of oviductal epithelial cells (Goldberg and Friedman, 1995;Joshi, 1995;Walter, 1995;Abe and Hoshi, 1997;Walker et al, 1997). In contrast, progesterone antagonizes the effect of estrogen on feline oviducts when estrogen and progesterone are administered sequentially (West et al, 1976).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of newly established clonal oviductal cell lines and differential hormonal reculation of gene expression

Umezu

Hanazono

Aizawa

et al. 2003

In Vitro Cell.Dev.Biol.-Animal

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Oviductal functions have been studied mainly in primary epithelial cell culture and organ culture. However, secretory cells and ciliated cells coexist in the epithelium, and the small size of the oviduct limits the sources of both epithelial and stromal cells. To circumvent the limits, we attempted to establish clonal cell lines from an oviduct of a p53-deficient mouse. An oviduct was enzymatically digested and cultured in medium containing 10% fetal calf serum supplemented with estradiol-17beta. Morphologically distinct clones (10 epithelial and 4 fibroblastic clones) were established, and all clones expressed estrogen receptor alpha and progesterone receptor. Expression of a mouse oviduct-specific glycoprotein gene as a marker of secretory cells was limited in one clone and was stimulated by estrogens and suppressed by progesterone. Expression of helix factor hepatocyte nuclear factor/forkhead homologue-4 gene as a marker of ciliated cells was limited in two clones and was suppressed by estrogens. The two genes were never coexpressed in any clones. The results strongly suggest that the oviductal epithelium consists of two functionally determined populations. To our knowledge, this is the first establishment of functional clonal cell lines of the oviduct and makes it possible to study independently two oviductal functions, secretion and ciliogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of newly established clonal oviductal cell lines and differential hormonal reculation of gene expression

Umezu

Hanazono

Aizawa

et al. 2003

In Vitro Cell.Dev.Biol.-Animal

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“…Eine Optimierung der Zellkulturen wird unter anderem von Co-Kulturen erwartet. Die Möglichkeit, relativ einfach tubare Epithelzellen zu gewinnen und zu kultivieren, rechtfertigt ihre Anwendung und weitere Untersuchungen über ihre Wirksamkeit in Co-Kulturen menschlicher Embryonen [126].…”

Section: Kulturunclassified

Möglichkeiten zur Vereinfachung und Rationalisierung der IVF-Behandlung - Entwicklungen und aktueller Stand

Nawroth¹,

Sudik²

1999

Geburtsh u Frauenheilk

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Die Vereinfachung und Rationalisierung der In-vitro-Fertilisation (IVF) sind Voraussetzungen, um mehr Patienten zur Durchführung der Behandlung zu ermutigen, die Abbruchrate nach erfolgloser IVF zu senken und durch wiederholte IVF-Versuche die kumulative Schwangerschaftsrate zu erhöhen. Sie führen zu einer Kostensenkung und könnten dadurch zu einer Sicherung der IVF als Leistung des öffentlichen Gesundheitswesens in Deutschland beitragen. Diese Arbeit gibt anhand der Literatur eine Übersicht über die wichtigsten Fortschritte zur Vereinfachung und Rationalisierung der IVF. AbstractSimplification and rationalisation of in-vitro fertilisation (IVF) are essential for encouraging patients for IVF, to decrease the "drop-out" rate after unsuccessful therapy and to increase the cumulative pregnancy rate by repeated IVF trials. This can decrease the cost and could make a contribution to secure IVF as a performance of public health in Germany. The paper surveys the most important advances to simplify and rationalise the IVF procedere in terms of the literature.

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“…Tubal cells have been reported to be harvested from the fallopian tubes of postpartum tubal ligations and hysterectomies. 30,31 To mimic the in vivo environment of the fallopian tube, especially that portion of the tube that is associated with fertilization and early embryo development, tubal cells from the ampulla portion of the fallopian tube have been used in co-culture ( Fig. 1).…”

Section: Tubalmentioning

confidence: 99%

Co-Culture Update: Creating an Embryotrophic Environment In Vitro

Conway-Myers

1998

Semin Reprod Med

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As infertility treatments evolve and techniques are developed to improve the fertilization and pregnancy rates, it is clear that the in vitro environment in which the gametes/embryos are cultured is less than perfect when compared to the in vivo counterpart. This becomes a serious problem for couples who seek to have children but are limited by unsuccessful attempts to become pregnant and a technology that is behind in mimicking the in vivo environment that would enhance gamete interaction and embryo development. In an attempt to begin to correlate the in vivo and in vitro microenvironment of the gametes/embryos and to recognize the potential of the fallopian tube as more then a highway for oocytes to transverse in their pathway to the uterus, coculturing of tubal cells along with gametes and embryos was introduced to the in vitro fertilization community. This report is an attempt to compile the research and results of those attempting to improve pregnancy rates by improving in vitro culture conditions via co-culture of cells with gametes and/or embryos.

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Establishment of human tubal epithelial cells for coculture in an IVF program

Cited by 7 publications

References 14 publications

Characterization of newly established clonal oviductal cell lines and differential hormonal reculation of gene expression

Characterization of newly established clonal oviductal cell lines and differential hormonal reculation of gene expression

Möglichkeiten zur Vereinfachung und Rationalisierung der IVF-Behandlung - Entwicklungen und aktueller Stand

Co-Culture Update: Creating an Embryotrophic Environment In Vitro

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