Establishment of hydrogen-utilizing bacteria in the rumen of the newborn lamb

Morvan, B.; Doré, J.; Rieu-Lesme, F.; Foucat, LoÃ ̄c; Fonty, Gérard; Gouet, Philippe

doi:10.1016/0378-1097(94)90567-3

Cited by 59 publications

(53 citation statements)

References 31 publications

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“…This suggested that consumption of the produced hydrogen is already occurring during the primary stages of development. However, this consumption is not necessarily linked to methanogenesis, as a previous study revealed the existence of a large population of hydrogenotrophic bacteria such as acetogens and sulfate‐reducing bacteria, which may compete for the hydrogen present, during early colonization (Morvan et al ., ; Fonty et al ., ).…”

Section: Resultsmentioning

confidence: 98%

Compositional and functional dynamics of the bovine rumen methanogenic community across different developmental stages

Friedman

Jami

Mizrahi

2017

Environmental Microbiology

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Methanogenic archaea in the bovine rumen are responsible for the reduction of carbon molecules to methane, using various electron donors and driving the electron flow across the microbial food webs. Thus, methanogens play a key role in sustaining rumen metabolism and function. Research of rumen methanogenic archaea typically focuses on their composition and function in mature animals, while studies of early colonization and functional establishment remain scarce. Here, we investigated the metabolic potential and taxonomic composition of the methanogenic communities across different rumen developmental stages. We discovered that the methanogenesis process changes with age and that the early methanogenic community is characterized by a high activity of methylotrophic methanogenesis, likely performed by members of the order Methanosarcinales, exclusively found in young rumen. In contrast, higher hydrogenotrophic activity was observed in the mature rumen, where a higher proportion of exclusively hydrogenotrophic taxa are found. These findings suggest that environmental filtering acts on the archaeal communities and select for different methanogenic lineages during different growth stages, affecting the functionality of this ecosystem. This study provides a better understanding of the compositional and metabolic changes that occur in the rumen microbiome from its initial stages of colonization and throughout the animals' life.

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Section: Resultsmentioning

confidence: 98%

Compositional and functional dynamics of the bovine rumen methanogenic community across different developmental stages

Friedman

Jami

Mizrahi

2017

Environmental Microbiology

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“…Hungate's method (1969) was used to prepare media and cultivate methanogens. Media were essentially the same as previously described (Morvan et al . 1994).…”

Section: Methodsmentioning

confidence: 94%

Effect of α‐cyclodextrin‐allyl isothiocyanate on ruminal microbial methane production in vitro

Lila¹,

Mohammed²,

Kanda³

et al. 2003

Animal Science Journal

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The objective of the present study was to investigate the effects of α‐cyclodextrin‐allyl isothiocyanate (CD‐AI) on ruminal microbial methane production and rumen fermentation of corn starch, soluble potato starch or hay plus concentrate (1.5:1) by mixed rumen microorganisms. Diluted rumen fluid (30 mL) was incubated anaerobically at 38°C for 6 and 24 h with or without CD‐AI (0, 0.4, 0.8, 1.6 and 3.2 g/L). The pH of the medium was unchanged by CD‐AI in all substrates. The molar proportion of acetate was decreased and propionate was increased with a corresponding decrease in acetate : propionate ratio (P < 0.05). Total volatile fatty acids and butyrate were increased (P < 0.05). Ammonia‐N was decreased (P < 0.05). Except with soluble potato starch, numbers of protozoa were unchanged after 6 h. As concentration of CD‐AI increased from 0 to 3.2 g/L, fermentation of corn starch, soluble potato starch and hay plus concentrate resulted in decreased (P < 0.05) methane production of 49–100% (6 h) and 14–100% (24 h); 39–100% (6 h) and 16–100% (24 h); and 45–100% (6 h) and 17–100% (24 h), respectively. When hay plus concentrate was used as substrate, methanogenic bacteria were decreased (P < 0.05) with 0.8 g/L of CD‐AI after 6 h. Excluding the lower dose level (0.4 g/L) of CD‐AI, digestibility of neutral detergent fiber of hay plus concentrate was decreased (P < 0.05) after 24 h. A suitable level of CD‐AI could therefore be used as a supplement to inhibit methane production and improve rumen fermentation without detrimental effects on fiber digestion.

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“…The established rumen microbiome in the young animal lays a solid foundation for the transition from the pre- to the ruminant state, a critical stage for later life. Many major functional groups of ruminal microorganisms—including cellulolytic and sulfate-reducing bacteria, as well as other hydrogen-utilizing species, such as acetogenic bacteria—become established in the rumen within the first week of life [8, 36]. The role played in this experiment by the acetogenic bacteria might partly explain the different response observed in does and kids treated with BCM.…”

Section: Discussionmentioning

confidence: 99%

“…The early establishment of acetogenic bacteria underlines the competition that exists between different H 2 utilizing species within the rumen. Morvan et al [8] observed that acetogens did colonize the rumen of lambs before methanogens did. Also Faichney et al [37] reported that lambs reared in isolation from their dams produced 30–40% less methane than conventional animals and H 2 appeared to be channeled via reductive acetogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…The developing rumen provides an opportunity to explore means of microbial manipulation. Methanogenic archaea have been found in the undeveloped rumen of lambs well before the ingestion of solid feed begins (2–4 days) and reach levels similar to those in adult animals around 10 days after birth [7, 8]. Skillman et al [9] suggested that the ewe might be the most important source of microbial inoculation in the young animal.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Antimethanogenic Nutritional Intervention in Early Life of Ruminants Modifies Ruminal Colonization by Archaea

Abecia

Waddams

Fernández

et al. 2014

Archaea

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The aim of this work was to study whether feeding a methanogen inhibitor from birth of goat kids and their does has an impact on the archaeal population colonizing the rumen and to what extent the impact persists later in life. Sixteen goats giving birth to two kids were used. Eight does were treated (D+) with bromochloromethane after giving birth and over 2 months. The other 8 goats were not treated (D−). One kid per doe in both groups was treated with bromochloromethane (k+) for 3 months while the other was untreated (k−), resulting in four experimental groups: D+/k+, D+/k−, D−/k+, and D−/k−. Rumen samples were collected from kids at weaning and 1 and 4 months after (3 and 6 months after birth) and from does at the end of the treating period (2 months). Pyrosequencing analyses showed a modified archaeal community composition colonizing the rumen of kids, although such effect did not persist entirely 4 months after; however, some less abundant groups remained different in treated and control animals. The different response on the archaeal community composition observed between offspring and adult goats suggests that the competition occurring in the developing rumen to occupy different niches offer potential for intervention.

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Establishment of hydrogen-utilizing bacteria in the rumen of the newborn lamb

Cited by 59 publications

References 31 publications

Compositional and functional dynamics of the bovine rumen methanogenic community across different developmental stages

Compositional and functional dynamics of the bovine rumen methanogenic community across different developmental stages

Effect of α‐cyclodextrin‐allyl isothiocyanate on ruminal microbial methane production in vitro

An Antimethanogenic Nutritional Intervention in Early Life of Ruminants Modifies Ruminal Colonization by Archaea

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