Establishment of in vitro culture systems for breeding new types of kiwifruit and for<i>Actinidia</i>genomics studies

Jiantao, Wu

doi:10.17660/actahortic.2018.1224.26

Cited by 4 publications

(4 citation statements)

References 33 publications

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“…Therefore, opposed to diploid plants, the decreased expression of proteolytic enzyme-related genes could contribute to more robust leaves in tetraploid plants. The transcriptomes would be determined by the full message RNA expression in which the transcription varies depending on many factors; the tetraploids with high transcriptions could be a response to the genomics interaction inside of the new high levels of genomics of the new organize, which could be a natural response [13].…”

Section: Discussionmentioning

confidence: 99%

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Induction, identification and genetics analysis of tetraploid Actinidia chinensis

Liu

et al. 2019

R. Soc. open sci.

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Actinidia chinensis is a commercially important fruit, and tetraploid breeding of A. chinensis is of great significance for economic benefit. In order to obtain elite tetraploid cultivars, tetraploid plants were induced by colchicine treatment with leaves of diploid A. chinensis ‘SWFU03’. The results showed that the best treatment was dipping leaves 30 h in 60 mg l−1 colchicine solutions, with induction rate reaching 26%. Four methods, including external morphology comparison, stomatal guard cell observation, chromosome number observation and flow cytometry analysis were used to identify the tetraploid of A. chinensis. Using the induction system and flow cytometry analysis methods, 187 tetraploid plants were identified. Three randomly selected tetraploid plants and their starting diploid plants were further subjected to transcriptome analysis, real-time quantitative polymerase chain reaction (RT-qPCR) and methylation-sensitive amplification polymorphism (MSAP) analysis. The transcriptome analysis results showed that there were a total of 2230 differentially expressed genes (DEG) between the diploid and tetraploid plants, of which 660 were downregulated and 1570 upregulated. The DEGs were mainly the genes involved in growth and development, stress resistance and antibacterial ability in plants. RT-qPCR results showed that the gene expression levels of the growth and stress resistance of tetraploid plants were higher than those of diploid ones at the transcriptome level. MSAP analysis of DNA methylation results showed that tetraploid plants had lower methylation ratio than diploid ones. The present results were valuable to further explore the epigenetics of diploid and tetraploid kiwifruit plants.

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Section: Discussionmentioning

confidence: 99%

“…In practice, colchicine induction of tetraploidy was commonly used with immersion or dropping method [11,12]. In vitro induction method has a lot of advantages, not only experimental conditions can be artificially controlled, easy operation, low cost, but also the results of the experiment can be replicated [13].…”

Section: Introductionmentioning

confidence: 99%

Induction, identification and genetics analysis of tetraploid Actinidia chinensis

Liu

et al. 2019

R. Soc. open sci.

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“…In our study, we observed that the age of the mother plant and size of the explant can determine the success of cryopreservation; use of younger donor plants and smaller explants significantly increasing regeneration. The response of kiwifruit plants to in vitro culture is not just species-specific, but also genotype-specific [ 32 ]. Hence, significant variation in post-cryopreservation regeneration between different genotypes was expected, and was observed to range from 59% to 88% [ 33 , 34 ].…”

Section: Use Of the In Vitro Kiwifruit Collection For Germplasm Conse...mentioning

confidence: 99%

Development, Management and Utilization of a Kiwifruit (Actinidia spp.) In Vitro Collection: A New Zealand Perspective

Nadarajan,

Esfandiari,

Mathew

et al. 2023

Plants

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The New Zealand Institute for Plant and Food Research Limited (PFR) supports a large kiwifruit breeding program that includes more than twenty Actinidia species. Almost all the kiwifruit accessions are held as field collections across a range of locations, though not all plants are at multiple locations. An in vitro collection of kiwifruit in New Zealand was established upon the arrival of Pseudomonas syringae pv. Actinadiae-biovar 3 in 2010. The value of an in vitro collection has been emphasized by restrictions on importation of new plants into New Zealand and increasing awareness of the array of biotic and abiotic threats to field collections. The PFR in vitro collection currently holds about 450 genotypes from various species, mostly A. chinensis var. chinensis and A. chinensis var. deliciosa. These collections and the in vitro facilities are used for germplasm conservation, identification of disease-free plants, reference collections and making plants available to users. Management of such a diverse collection requires appropriate protocols, excellent documentation, training, sample tracking and databasing and true-to-type testing, as well as specialized facilities and resources. This review also discusses the New Zealand biosecurity and compliance regime governing kiwifruit plant movement, and how protocols employed by the facility aid the movement of pathogen-free plants within and from New Zealand.

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“…Recently, more and more research studies on the establishment of kiwifruit rapid propagation systems have been performed [6][7][8][9]. However, in the process of in vitro rapid propagation of kiwifruit, serious browning and diseases of explants in primary culture have become a major obstacle [10,11]. In recent years, some literature has reported that many viruses, including apple stem grooving virus (ASGV), cucumber mosaic virus (CMV), Actinidia virus X (AVX), cucumber necrosis virus (CNV), ribgrass mosaic virus (RMV), Actinidia virus B (AcVB), Actinidia virus A (AcVA), Actinidia virus 1 (AcV-1), alfalfa mosaic virus (AMV), cherry leaf roll virus (CLRV), tomato necrotic spot associated virus (TNSaV), tomato zonate spot virus (TZSV), Pelargonium zonate spot virus (PZSV), citrus leaf blotch virus (CLBV), Actinidia chlorotic ringspot-associated virus (AcCRaV), turnip vein clearing virus (TVCV), and Actinidia seed-borne latent virus (ASbLV), can infect kiwifruit plants through pollen, grafting, or artificial inoculation [12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang”

Zhong

Zhou

Tang

et al. 2021

Journal of Chemistry

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In order to breed virus-free plantlets of the kiwifruit cultivar “Guichang,” which belongs to Actinidia deliciosa, in this study, stem segments with buds were used as explants, the establishment of a tissue culture rapid propagation system was carried out, and then the virus status of tissue culture plantlets was detected via the real-time reverse transcription-polymerase chain reaction (RT-qPCR) method. The tissue culture rapid propagation system proved that the contamination and browning rates could be controlled below 20% and the survival rate could be exceeded by 70% when the single bud stem segment of “Guichang” kiwifruit was sterilized with 70% alcohol for 30–60 s and 15% NaClO for 15 min, respectively. Meanwhile, we screened the hormone concentration to get better results, and the appropriate medium for adventitious bud induction was MS + 6-BA (1.0 mg/L) + IBA (0.2 mg/L); for proliferation, it was MS + 6-BA (1.0 mg/L) + IBA (0.1 mg/L); and for rooting, it was 1/2 MS + IBA (0.3 mg/L), and the efficiency of induction, proliferation, and rooting could reach 74.07%, 79.63%, and 85.18%, respectively. In addition, the RT-qPCR results demonstrated that the infection rate of 9 viruses: apple stem grooving virus (ASGV), cucumber mosaic virus (CMV), Actinidia virus X (AVX), cucumber necrosis virus (CNV), ribgrass mosaic virus (RMV), citrus leaf blotch virus (CLBV), Actinidia virus B (AcVB), Pelargonium zonate spot virus (PZSV), and cherry leaf roll virus (CLRV) in the “Guichang” kiwifruit tissue culture plantlets was 0. This study could lay a foundation for the production of “Guichang” kiwifruit tissue culture seedlings, and the medium formula provided in this study was useful for the industrial rapid propagation of “Guichang” plantlets.

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Establishment of in vitro culture systems for breeding new types of kiwifruit and forActinidiagenomics studies

Cited by 4 publications

References 33 publications

Induction, identification and genetics analysis of tetraploid Actinidia chinensis

Induction, identification and genetics analysis of tetraploid Actinidia chinensis

Development, Management and Utilization of a Kiwifruit (Actinidia spp.) In Vitro Collection: A New Zealand Perspective

Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang”

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