Establishment of male-specific epigenetic information

Rousseaux, Sophie; Caron, Cécile; Govin, Jérôme; Lestrat, Cécile; Faure, Anne‐Karen; Khochbin, Saadi

doi:10.1016/j.gene.2004.12.004

Cited by 148 publications

(129 citation statements)

References 187 publications

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“…The chromatin in mature sperm nuclei is highly condensed, often as a consequence of association with sperm-specific packaging proteins such as protamines (reviewed in Poccia and Collas, 1996; Jayaramaiah Raja and Renkawitz-Pohl, 2005) or sperm-specific histones (reviewed in Imschenetzky et al, 2003). This tight packaging has been postulated to streamline sperm for motility, protect sperm DNA from physical and chemical damage, and reset somatic histone epigenetic modifications (reviewed in Poccia and Collas, 1996;Rousseaux et al, 2005). Such tight packaging also results in cessation of gene expression and DNA replication (Poccia and Collas, 1996).…”

Section: Conversion Of the Sperm Nucleus Into A Male Pronucleusmentioning

confidence: 99%

Transitioning from egg to embryo: Triggers and mechanisms of egg activation

Horner

Wolfner

2008

Developmental Dynamics

183

177

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The transition from mature oocyte to developing embryo requires a coordinated series of events, collectively known as egg activation. Egg activation includes changes to egg coverings to prevent polyspermy, release of oocyte meiotic arrest, generation of haploid female and male pronuclei, changes in maternal mRNAs and protein populations, and cytoskeletal rearrangements. In many animals, egg activation is triggered by fertilization, which increases intracellular calcium within the oocyte and thereby regulates molecular events of egg activation. In other animals, fertilization-independent external signals, including mechanical stimulation of eggs and/or changes in ionic milieu, trigger activation. Recent studies have clarified the upstream portion of pathways leading to eggshell changes and cell cycle resumption and have identified activation-induced changes in maternal mRNA and protein profiles that can identify molecular players in the downstream events of egg activation. We review signals that trigger activation and how they link to subsequent molecular events of egg activation. Developmental Dynamics 237:527-544, 2008.

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Section: Conversion Of the Sperm Nucleus Into A Male Pronucleusmentioning

confidence: 99%

Transitioning from egg to embryo: Triggers and mechanisms of egg activation

Horner

Wolfner

2008

Developmental Dynamics

183

177

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“…Differentiation of spermatogonia cells during spermatogenesis within testis is particularly controlled by posttranscriptional modifications [41].…”

Section: Introductionmentioning

confidence: 99%

TBHP-induced oxidative stress alters microRNAs expression in mouse testis

Fatemi

Sanati

Shamsara

et al. 2014

J Assist Reprod Genet

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Purpose Reactive oxygen species (ROS) and oxidative stress is one of the main reasons of male infertility. MicroRNAs (miRNAs) regulate multiple intracellular processes. Alterations in miRNAs expression may occur in different conditions and diseases. In this study, the effect of oxidative stress induced by tertiary-butyl hydroperoxide (TBHP) on the expression of candidate miRNAs in mouse testis was investigated.Methods After determining median lethal dose (LD 50 ), TBHP was intraperitoneally (ip) injected at the dilution of 1:10 LD 50 into the adult male mice for 2weeks, and then testis tissues were removed in order to assay the ROS level. Total RNA was extracted and the expression of five miRNAs was quantified by reverse transcription-real time polymerase chain reaction (RT-qPCR).Results The flow cytometry analysis showed a significant increase in ROS level in testis. The expression of three out of five selected miRNAs, including miR-34a, miR-181b and miR-122a, showed some degrees of changes following exposure to oxidative stress. These miRNAs are involved in antioxidant responses, inflammation pathway and spermatogenesis arrest. Conclusions In conclusion, TBHP alters the miRNA expression profile of testis which might play a potential role in oxidative and antioxidative responses and spermatogenesis.

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“…The human spermatozoon is a highly specialized cell with extremely marked compositional, morphological and functional differences, as compared to other somatic or germinal cells [1][2][3][4]. For example, the sperm nucleus is condensed by protamines into a highly compact and hydrodynamic sperm head, and the cell is provided with a large flagellum to allow its motility [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

“…For example, the sperm nucleus is condensed by protamines into a highly compact and hydrodynamic sperm head, and the cell is provided with a large flagellum to allow its motility [1][2][3][4]. In addition to the importance of the study of the sperm proteins to understand the fundamental aspects of reproduction, it is also relevant towards the identification of the causes of human infertility.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic identification of human sperm proteins

Martínez-Heredia¹,

Estanyol²,

Ballescà³

et al. 2006

Proteomics

233

177

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Conventional 1-DE has in the past provided a wealth of information concerning the major sperm proteins. However, so far there are relatively few reports exploiting the potential of the present proteomic tools to identify and to study additional yet-unidentified important proteins present in human spermatozoa. In the present work, 2-DE of proteins extracted from human normozoospermic spermatozoa led to the resolution of over 1000 spots. Subsequent excision from the gels of 145 spots and MALDI-TOF MS analysis allowed the identification of 98 different proteins. The function of these proteins turned out to be energy production (23%), transcription, protein synthesis, transport, folding and turnover (23%), cell cycle, apoptosis and oxidative stress (10%), signal transduction (8%), cytoskeleton, flagella and cell movement (10%), cell recognition (7%), metabolism (6%) and unknown function (11%). As many as 23% of the proteins identified have not been previously described as being expressed in human spermatozoa. The present data provide an important clue towards determining the function of these proteins and opens up the possibility to perform additional experiments.

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Establishment of male-specific epigenetic information

Cited by 148 publications

References 187 publications

Transitioning from egg to embryo: Triggers and mechanisms of egg activation

Transitioning from egg to embryo: Triggers and mechanisms of egg activation

TBHP-induced oxidative stress alters microRNAs expression in mouse testis

Proteomic identification of human sperm proteins

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