Establishment of mice model with human viral hepatitis B

Abstract

AIM:To establish a mice model of hepatitis B by using HBV-transgenic mice, and to transfer HBV-specific cytotoxic T lymphocytes (CTL) induced from syngeneic BALB/c mice immunized by a eukaryotic expression vector containing HBV complete genome DNA.

Preserved Function of Circulating Invariant Natural Killer T Cells in Patients With Chronic Hepatitis B Virus Infection

“…458 Hospital (China), which expresses high level of HBsAg and have detectable HBV DNA in the serum. 20 The same weeks of BALB/c mice were purchased as control mice. The full-HBV-Tg mice were fed in the laboratory in accordance with Biological Safe Level 2.…”

Impairment of liver regeneration correlates with activated hepatic NKT cells in HBV transgenic mice

“…The transgene in these mice consists of 1.3 copies of the HBV adr complete genome. The full‐HBV‐tg mice express high level of HBsAg in their serum and have detectable HBV DNA in their serum 15. B6 mice or BALB/c mice were purchased from Shanghai Experimental Animal Center, Chinese Academy of Science (Shanghai, China) as respective control mice.…”

Cell and Animal Models for Studying Hepatitis B Virus Infection and Drug Development

“…64 However, infected mice can be used to study features of the innate immune response, such as HBV inhibition of interferon and tumor necrosis factor. 85,86 Adoptive transfer of immune cells from syngeneic mice immunized against HBV can be used to study the adaptive immune response. These mice do not develop liver fibrosis or cirrhosis, and only a few strains of transgenic mice, such as those that express large HBsAg or overexpress HBx protein, develop HCC.…”