Establishment of monokaryotic and dikaryotic isolates of Hedgehog mushrooms (Hydnum repandum and related species) from basidiospores

Sugawara, Ryo; Yamada, Akiyoshi; Kawai, Masataka; Sotome, Kozue; Maekawa, Nitaro; Nakagiri, Akira; Endo, Naoki

doi:10.1016/j.myc.2019.02.007

Cited by 9 publications

(7 citation statements)

References 37 publications

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“…To obtain living culture strains monosporous isolations was carried out following to Sugawara et al (2019) . When enough basidiospores were not fallen, we directly isolated cultures from fallen spores on culture medium (multisporous isolation).…”

Section: Methodsmentioning

confidence: 99%

Two new species of Sistotrema s.l. (Cantharellales) from Japan with descriptions of their ectomycorrhizae

et al. 2022

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We describe two new species of resupinate Sistotrema sensu lato ( Cantharellales ) collected in Japan: S. flavorhizomorphae and S. chloroporum . Both species have urniform basidia with more than four sterigmata and monomitic hyphal system, oil-rich hyphae in subiculum, which is typical for this genus. Sistotrema chloroporum is characterized by poroid hymenophore partly yellowish-green, basidia 4-6-spored, medium-sized basidiospores (4.5-6.5 × 3.5-6 µm), and broadleaf forest habitat. Sistotrema flavorhizomorphae is characterized by hydnoid-irpicoid hymenophore, bright yellowish rhizomorphs, basidia 6-8-spored, small basidiospores (3-3.5 × 2.5-3 µm), and pine forest habitat. Phylogenetic trees inferred from the fungal nrDNA ITS and LSU and the rpb2 sequences supported that both species were distinct and grouped with other ectomycorrhizal Sistotrema and Hydnum species, but their generic boundary was unclear. Mycorrhizae underneath basidiomes of both species were identified and described via molecular techniques. Mycorrhizae of S. chloroporum have similar characteristics to those of other Sistotrema s.l. and Hydnum species, i.e., S. confluens and H. repandum , whereas S. flavorhizomorphae has a distinct morpho-anatomy, for example, a distinct pseudoparenchymatous mantle. Comprehensive characterizations of basidiomes and mycorrhizae improve the taxonomic analysis of mycorrhizal species of Sistotrema s.l.

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Section: Methodsmentioning

confidence: 99%

Two new species of Sistotrema s.l. (Cantharellales) from Japan with descriptions of their ectomycorrhizae

et al. 2022

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“…We examined 33 basidiomata specimens of H. boreorepandum and H. repando-orientale s. lat. (Table 1): 23 were collected in our studies in 2016-2020 (Sugawara et al 2019(Sugawara et al , 2022a); 4 were collected in this study; 2, including holotype of H. repando-orientale (TUMH 60745), were loaned from the Tottori University Mycological Herbarium (TUMH), Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University; 4 were loaned from the TNS herbarium of the National Museum of Nature and Sciences, Tokyo. These specimens were collected at 25 sites in Honshu, mainland Japan, at 10 to 2500 m a.s.l.…”

Section: Basidiomata Specimensmentioning

confidence: 99%

Section: Monospore Isolationmentioning

confidence: 99%

“…Fresh basidiomata materials were used for monospore isolation, in accordance with the method proposed by Sugawara et al (2019). Basidiospores from each hymenophore were collected on an axenic plastic Petri dish, suspended in sterile distilled water, and inoculated onto modi ed Norkrans' C medium (MNC; Yamada and Katsuya 1995) solidi ed with 1.5% gellan-gum (MNC1.5G; Sugawara et al 2019). Using a platinum loop, inoculated spores were streaked in zigzag to create a spore concentration gradient; they were then incubated at 15°C in the dark (MIR-254-PJ, Panasonic Healthcare, Tokyo, Japan).…”

Section: Monospore Isolationmentioning

confidence: 99%

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Ecological speciation of Japanese hedgehog mushroom: Hydnum subalpinum sp. nov. is distinguished from its sister species H. repando-orientale by means of integrative taxonomy

et al. 2022

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Hydnum repando-orientale is an East Asian species closely related to H. boreorepandum and H. repandum; all three species produce edible mushrooms. We identi ed two ecological groups of H. repando-orientale in Japan: a temperate group occurring in Fagaceae-dominated forest at < 1200 m a.s.l. (ROF), and a subalpine group occurring in coniferous forest in highland at > 1900 m a.s.l. (ROC). We re-examined the taxonomy of the two ecological groups of H. repando-orientale using integrative approaches. Phylogenies of the two ecological groups and other related species were inferred from the internal transcribed spacer (ITS) and gene portions encoding the large subunit of nc rRNA (LSU), translation elongation factor-1 alpha (TEF1), RNA polymerase II largest subunit (RPB1), and RNA polymerase II second-largest subunit (RPB2).

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“…Mycelial inoculum is usually obtained by propagating the flesh of EMF fruiting bodies ( Brundrett et al, 1996 ; Daza et al, 2006 ) and more rarely from spores ( Ohta, 1986 ; Murata et al, 2015 ). Recently, however, field-collected ectomycorrhizae ( Ogawa et al, 2019a ; Endo et al, 2020 ) and spores ( Yamada et al, 2019 ; Sugawara et al, 2019 ) have been shown to provide valuable alternative sources of pure mycelial cultures. Nutrient-rich media, having high concentrations of exogenous glucose (1–20 g/L), are often required to sustain mycelial growth ( Gibson & Deacon, 1990 ; Hutchison & Piché, 1994 ; Yamada, Ogura, & Ohmasa, 2001a ; Daza et al, 2006 ).…”

Section: Controlled Mycorrhizal Synthesismentioning

confidence: 99%

Successes and challenges in the sustainable cultivation of edible mycorrhizal fungi – furthering the dream

Guerin‐Laguette

2021

Mycoscience

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Edible mycorrhizal fungi (EMF) include truffles (Périgord black truffle, Italian white truffle etc.) and other highly prized forest mushrooms (porcini, chanterelles, matsutake etc.). Most edible EMF truffle species belong to Ascomycota, while EMF mushrooms belong to Basidiomycota. They are abundant in boreal and temperate forests, where they live in ectomycorrhizal symbiosis with trees, colonizing their roots and transforming them into well-defined ectomycorrhizae, mixed organs where fungal and plant tissues are merged. EMF are, however, also present in dry and desert areas, e.g. the Mediterranean Basin, where the so-called 'desert truffles' engage in a polyvalent mycorrhizal relationship with Cistaceae Juss. of the genus Helianthemum Mill. (Morte, Gutiérrez, & Navarro Ródenas, 2020). Mycorrhizae enable both partners to live together and exchange mutual services (Smith & Read, 2008). The fungus supplies the tree with water and nutrients that its extensive mycelium, made of microscopic hyphae, pumps or mobilizes from the soil. In exchange, the tree, or shrub, reallocates photosynthates to its fungal symbiont, which in most cases, are otherwise inaccessible to EMF, given their inability to decompose carbohydrate polymers such as starch, cellulose or lignin. From a practical point of view, this symbiotic relationship leads to (1) fast-growing and resilient mycorrhizal host trees (

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Establishment of monokaryotic and dikaryotic isolates of Hedgehog mushrooms (Hydnum repandum and related species) from basidiospores

Cited by 9 publications

References 37 publications

Two new species of <i>Sistotrema</i> s.l. (<i>Cantharellales</i>) from Japan with descriptions of their ectomycorrhizae

Two new species of <i>Sistotrema</i> s.l. (<i>Cantharellales</i>) from Japan with descriptions of their ectomycorrhizae

Ecological speciation of Japanese hedgehog mushroom: Hydnum subalpinum sp. nov. is distinguished from its sister species H. repando-orientale by means of integrative taxonomy

Successes and challenges in the sustainable cultivation of edible mycorrhizal fungi – furthering the dream

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