Establishment of national reference for bunyavirus nucleic acid detection kits for diagnosis of SFTS virus

Xu, Lixin; Dongting, Bai; Li, Yuhua

doi:10.1186/s12985-017-0682-z

Cited by 4 publications

(4 citation statements)

References 21 publications

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“…Serum samples from suspected SFTS patients and identified matched controls were collected for testing. RT‐PCR detection was used to detect SFTSV RNA, as previously described 15 . SFTSV‐specific IgM and IgG antibodies in sera of suspected patients were examined with a commercial GICA kit (Zhongshan Bio‐Tech Co., Ltd.) according to the manufacturer's instructions 16 …”

Section: Methodsmentioning

confidence: 99%

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Severe fever with thrombocytopenia syndrome in Hefei: Clinical features, risk factors, and ribavirin therapeutic efficacy

Zhang

Miao

et al. 2020

Journal of Medical Virology

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Objectives This study described the clinical features, risk factors, factors affecting the outcome of this disease, and ribavirin therapeutic efficacy for severe fever with thrombocytopenia syndrome (SFTS) patients in Hefei. Methods Between April 2020 and July 2020, 62 cases admitted to the First Affiliated Hospital of Anhui Medical University were included in this study. Serum samples were collected from all patients, after which diagnosis was made via reverse transcription‐polymerase chain reaction and via the use of a colloidal gold immunochromatography assay approach. Results In multivariate analysis, the following factors were determined as risk factors for SFTS: Being a farmer (odds ratio [OR], 3.033), working in areas with weeds and shrubs (OR, 2.807), and being bitten by a tick (OR, 6.64). The rates of confusion, neck stiffness, viral encephalopathy, and the presence of liver damage were higher in the patients who died than that in the surviving ones. Additionally, the median of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, creatine phosphokinase, activated partial thromboplastin time, D‐dimer, fibrinogen degradation products, creatinine, and urea was also higher in the patients who died. One of the 15 patients treated with ribavirin in the early stage could not survive (6.7%), whereas 11 of the 35 patients treated with ribavirin in the late stage could not survive (31.4%); this difference was statistically significant. However, there was no significant difference in mortality between the untreated group and the other two groups (i.e., patients who started antiviral treatment <5 days from the onset and those who started antiviral treatment ≥5 days from the onset). Moreover, there was no positive effect determined on clinical or laboratory parameters in SFTS patients treated with ribavirin. Also, it was observed that leukocyte levels and platelet levels took longer to return to normal. Conclusions In Hefei, clinical features, prognostic factors, and risk factors associated with SFTS are similar to those in other areas. Patients who were given ribavirin did not have better survival rates than patients who were not given ribavirin.

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Section: Methodsmentioning

confidence: 99%

“…The q‐PCR procedure was as follows: 50°C for 15 min, 94°C for 15 min; 45 cycles of 94°C for 15 s and 55°C for 45 s. The primers and probes located in the L, M, and S segments of SFTSV were obtained from a previous study. The cut‐off cycle threshold value was set at 35 cycles 15 …”

Section: Methodsmentioning

confidence: 99%

Severe fever with thrombocytopenia syndrome in Hefei: Clinical features, risk factors, and ribavirin therapeutic efficacy

Zhang

Miao

et al. 2020

Journal of Medical Virology

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“…У 5 штаммов -LN2012-14, LN2012-34, LN2012-41, LN2012-42 и AHL -обнаружена реассортация в S-сегменте. Таким образом, полученные данные свидетельствуют о том, что для ВД наличие реассортантных вариантов является частым событием [24].…”

Section: установление явления реассортации для вирусаunclassified

“…Скорость эволюции генома ВД оценена как 2,28 × 10 -4 , 2,42 × 10 -4 и 1,19 × 10 -4 нуклеотидных замен/ сайт/год для S-, M-и L-сегментов генома соответственно. Следовательно, скорость эволюции сегментов генома РНК ВД различна, будучи максимальной для сегмента M и минимальной -для L [24]. L. Liu и соавт.…”

Section: изучение особенностей эволюции вируса дабиunclassified

The molecular evolution of Dabie bandavirus (Phenuiviridae: Bandavirus: Dabie bandavirus), the agent of severe fever with thrombocytopenia syndrome

Сизикова¹,

Лебедев²,

Borisevich³

2021

Problems of Virology

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Since the Dabie bandavirus (DBV; former SFTS virus, SFTSV) was identified, the epidemics of severe fever with thrombocytopenic syndrome (SFTS) caused by this virus have occurred in several countries in East Asia. The rapid increase in incidence indicates that this infectious agent has a pandemic potential and poses an imminent global public health threat.The analysis of molecular evolution of SFTS agent that includes its variants isolated in China, Japan and South Korea was performed in this review. The evolution rate of DBV and the estimated dates of existence of the common ancestor were ascertained, and the possibility of reassortation was demonstrated.The evolutionary rates of DBV genome segments were estimated to be 2.28 × 10-4 nucleotides/site/year for S-segment, 2.42 × 10-4 for M-segment, and 1.19 × 10-4 for L-segment. The positions of positive selection were detected in the viral genome.Phylogenetic analyses showed that virus may be divided into two clades, containing six different genotypes. The structures of phylogenetic trees for S-, M- and L-segments showed that all genotypes originate from the common ancestor.Data of sequence analysis suggest that DBV use several mechanisms to maintain the high level of its genetic diversity. Understanding the phylogenetic factors that determine the virus transmission is important for assessing the epidemiological characteristics of the disease and predicting its possible outbreaks.

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Laboratory detection and molecular phylogenetic analysis of severe fever with thrombocytopenia syndrome virus in Hubei Province, central China

Cai

Liu

et al. 2018

Arch Virol

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Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by the SFTS virus (SFTSV). Hubei Province is a major epidemic area for SFTS in China. In this study, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and serological testing (IgM) were used simultaneously for laboratory detection of SFTS; however, testing results showed poor consistency between these two methods. Further analysis revealed that time post-onset was the main factor leading to inconsistent results. Thus, qRT-PCR is unable to detect all SFTS cases, and serological testing is essential. Here, 15 strains of SFTSV were successfully isolated from serum samples of acute SFTSV infection and their complete genomes were sequenced and submitted to GenBank. Phylogenetic analysis showed that the 15 SFTS virus strains clustered into four independent genotypes (A, B, D, and E), demonstrating that at least four genotypes of SFTSV have been co-circulating in Hubei Province. Furthermore, four strains of our isolates (HB2014-31, HB2014-35, HB2014-36, and HB2014-37) clustered in genotype E, which was the predominant genotype in Japan and South Korea. In this study, we identified multiple co-prevalent genotypes and confirmed the existence of genotype E viruses circulating in the Dabie Mountains of Hubei, central China. We concluded that SFTSV strains from Hubei exhibit most of the genetic diversity found in China.

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Establishment of national reference for bunyavirus nucleic acid detection kits for diagnosis of SFTS virus

Cited by 4 publications

References 21 publications

Severe fever with thrombocytopenia syndrome in Hefei: Clinical features, risk factors, and ribavirin therapeutic efficacy

Severe fever with thrombocytopenia syndrome in Hefei: Clinical features, risk factors, and ribavirin therapeutic efficacy

The molecular evolution of Dabie bandavirus (Phenuiviridae: Bandavirus: Dabie bandavirus), the agent of severe fever with thrombocytopenia syndrome

Laboratory detection and molecular phylogenetic analysis of severe fever with thrombocytopenia syndrome virus in Hubei Province, central China

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