Establishment of new human prostatic cancer cell line (JCA-I)

Evidence That Arachidonate 15-Lipoxygenase 2 Is a Negative Cell Cycle Regulator in Normal Prostate Epithelial Cells

“…MDA PCa 2a and 2b cell lines (abbreviated as 2a and 2b, respectively) (28) were derived from primary cultures of bone marrow metastases from a single patient. LNCaP (29), an androgen-responsive cell line and its androgen-independent sublines (C4-2 and C5 (30), PC3 (31), Du145 (32), JCA-1 (33), and Tsu-Pr (34) are all established metastatic cell lines. All cancer cell lines were cultured in RPMI 1640 supplemented with 5% heat-inactivated fetal bovine serum (FBS) and antibiotics.…”

Expression of Estrogen Receptor (ER–α and ER–β) mRNA in Human Prostate Cancer

“…Four different cell lines derived from human prostate cancer were used: LNCap as androgen–dependent cells [9], JCA–1 as untreated (before therapy) prostate cancer cells [10], and DU–145 [11]and PC–3 [12]as androgen–independent cells. As ER–positive control cells we used MCF–7, derived from human breast cancer [13], and HOS–TE 85, derived from human osteosarcoma cells [13].…”

Prevention of Cancer Cachexia by a Novel Nuclear Factor κB Inhibitor in Prostate Cancer

“…Cell line. JCA-1 cells derived from human prostate cancer (17) were grown in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum, 100 Ag/mL streptomycin (Life Technologies, Inc., Grand Island, NY), and 100 IU/mL penicillin (Life Technologies).…”