Establishment of novel meniscal scaffold structures using polyglycolic and poly-<scp>l</scp>-lactic acids

Murakami, Tomohiko; Otsuki, Shuhei; Nakagawa, Kosuke; Okamoto, Yukiko; Inoue, Takato; Sakamoto, Yuki; Sato, Hideki; Neo, Masashi

doi:10.1177/0885328217713631

Cited by 24 publications

(22 citation statements)

References 45 publications

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“…10,32 We previously evaluated several types of PGA scaffolds for use in meniscal implants and found that PGA covered with polylactic acid/caprolactone, or P(LA/CL), exhibited the highest biomechanical strength and least inflammation in a rabbit model. 20 The purpose of this study was to evaluate meniscal regeneration and prevention of cartilage degeneration as well as the degree of inflammation in a porcine model of meniscal injury.…”

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Evaluation of Meniscal Regeneration in a Mini Pig Model Treated With a Novel Polyglycolic Acid Meniscal Scaffold

et al. 2019

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Background: Meniscal injury is a severe impediment to movement and results in accelerated deterioration of the knee joint. Purpose: To evaluate the effect of a novel meniscal scaffold prepared from polyglycolic acid coated with polylactic acid/caprolactone on the treatment of meniscal injury in a mini pig model. Study Design: Controlled laboratory study. Methods: The model was established with a 10-mm resection at the anterior medial meniscus on both knee joints. A scaffold was implanted in the right knee joint. The meniscal scaffold was inserted and sutured next to the native meniscus. The histological analysis was performed to determine meniscal regeneration with safranin O staining, cell proliferation with PCNA, inflammation with TNF, and collagen structure and production with picrosirius red and immunofluorescence. Cartilage degeneration was evaluated with Safranin O. Meniscal regeneration and joint fluid were evaluated with magnetic resonance imaging. Results: Although compressive stress and elastic modulus were significantly lower in the scaffold than in the native porcine menisci, ultimate tensile stress was similar. Implanted scaffolds were covered with tissue beginning at 4 weeks, with increased migration of proliferating cells to the implant area at 4 and 8 weeks. Scaffolds were absorbed with freshly produced collagen at 24 weeks. Cartilage degeneration was significantly lower in the meniscus-implanted group than in the meniscectomy group. Magnetic resonance imaging results did not show severe accumulation of joint fluids, suggesting negligible inflammation. Density of the implanted menisci was comparable with that of the native menisci. Conclusion: Meniscal scaffold prepared from polyglycolic acid has therapeutic potential for meniscal regeneration. Clinical Relevance: This meniscal scaffold can improve biological knee reconstruction and prevent the increase of total knee arthroplasty.

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Evaluation of Meniscal Regeneration in a Mini Pig Model Treated With a Novel Polyglycolic Acid Meniscal Scaffold

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“…Outro aspecto importante é que o arcabouço deve possuir funções biomecânicas que atenderão as necessidades do paciente (16,17,50). Isto deve ocorrer até o fim do processo de regeneração tissular(3).…”

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“…Por envolver testes em seres vivos, as análises in vivo devem estar de acordo com protocolos de ética estabelecidos pelas instituições de pesquisa. As publicações analisadas escolheram em sua grande maioria modelos de animais pequenos, como coelhos (1,17,49,50,58,59) e camundongo (19). DEPONTI et al (2013)(2) concluem que do ponto de vista anatômico o menisco caprino é o mais semelhante ao humano e que outros modelos interessantes são os suíno e os ovinos.…”

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“…Os principais testes empregados para a caracterização da performance mecânica são: testes de tração e de compressão(1,3,4,17,18,23,29,30,33,50,51,53,54,58,59).Outro fator importante para o desenvolvimento de um scaffold é a porosidade em sua estrutura, pois influencia na promoção da aderência e proliferação celular. Ela está ligada com a tendência de formação tecidual no interior do arcabouço(9,16,17,32,38).…”

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Engenharia no Século XXI - Volume 4

Tinô¹,

Batista²,

Fontes³

et al. 2019

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“…Although PLLA can be degraded and has certain supporting performance, its supporting performance is still poor than that of metal scaffolds due to its own limitations. Simultaneously, the lactic acid metabolites produced during the degradation of the PLLA composite will cause long-term chronic stimulation to the vascular tissues around the scaffold, which will induce and aggravate the inflammatory reaction and delay the healing of blood vessels [5,6]. In addition, increasingly more advanced thrombus formation and stent restenosis are more challenging for single PLLA scaffold in clinical trials.…”

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Evaluation of Long-Term Inflammatory Responses after Implantation of a Novel Fully Bioabsorbable Scaffold Composed of Poly-L-lactic Acid and Amorphous Calcium Phosphate Nanoparticles

Gao

Nguyen

et al. 2018

Journal of Nanomaterials

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Objectives. Our previous studies have confirmed the superior biocompatibility of the poly-L-lactic acid/amorphous calcium phosphate (PLLA/ACP) scaffolds compared to PLLA scaffolds at 1-month. In the present study, the long-term inflammatory responses of PLLA/ACP scaffolds in a porcine coronary model have been explored. Methods. The 24 PLLA scaffolds and 24 PLLA/ACP scaffolds were implanted into the coronary arteries of 24 miniature pigs. Serum levels of ALT, AST, and CRP were measured before operation, as well as 1-month, 6-months, 12-months, and 24-months. The vascular segments were taken for pathomorphological observation. HE staining was used for the inflammatory score and fibrosis score. Immunohistochemical staining detected positive expression indexes of MMP-9 and NF-κB. The expression of inflammation-related proteins of IL-1 and IL-6 was detected by Western Blot in surrounding tissues of scaffolds. Results. There was no significant difference between the two groups in ALT, AST, and UR at different time points (P < 0 05). The inflammation score in the PLLA/ACP group was lower than that in the PLLA group at 6-months, 12-months, and 24-months (P < 0 05), and the fibrosis score was reduced in the PLLA/ACP group than that in the PLLA group at 12-months and 24-months (P < 0 05). The expression of MMP-9 and NF-κB in the PLLA/ACP group was significantly less than that in the PLLA group at 6-months, 12-months, and 24-months (P < 0 05). The protein expression of IL-1 in the PLLA/ACP group was decreased than that in the PLLA group at 12-months and 24-months (P < 0 05). Furthermore, the protein expression of IL-1 was significantly lower than that in the PLLA group at 6-months, 12-months, and 24-months (P < 0 01). Conclusions. The supplement of ACP nanoparticles can effectively reduce the long-term inflammatory reaction caused by PLLA and has good safety and biocompatibility. The novel bioabsorbable PLLA/ACP scaffold provides reliable guidance for the development and clinical application of bioabsorbable scaffolds in the future.

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Establishment of novel meniscal scaffold structures using polyglycolic and poly-l-lactic acids

Cited by 24 publications

References 45 publications

Evaluation of Meniscal Regeneration in a Mini Pig Model Treated With a Novel Polyglycolic Acid Meniscal Scaffold

Evaluation of Meniscal Regeneration in a Mini Pig Model Treated With a Novel Polyglycolic Acid Meniscal Scaffold

Engenharia no Século XXI - Volume 4

Evaluation of Long-Term Inflammatory Responses after Implantation of a Novel Fully Bioabsorbable Scaffold Composed of Poly-L-lactic Acid and Amorphous Calcium Phosphate Nanoparticles

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