Establishment of odontoblastic cells, which indicate odontoblast features both in vivo and in vitro

Abstract

Tooth tissue engineering offers very attractive perspectives for elaboration of regenerative treatments, which enables to cure tooth loss and restore quality of life of the patients. To elaborate such treatment, isolation and culture of dental pulp cell must be achieved as a key element. In this article, we report the establishment of a stable cell line from GFP transgenic rat dental pulp, named TGC (Tooth Matrix-forming, GFP Rat-derived Cell). TGCs have exhibited odontoblastic feature both in vitro and in vivo. In vitro, TGC exposed to osteogenic medium demonstrated collagen fiber synthesis with matrix vesicle and mineralization and formed a sheet-like substrate on the cell culture dish. Increased ALP activity and elevated transcription level of various genes involved in calcification and dentin formation were also observed. In vivo, transplanted TGC in SCID mice with β-TCP particles formed dentin-like and pulp-like structure with lining odontoblast. Notably, even after up to 80 passages, TGCs retain their morphological features and differentiation ability. To our knowledge, this is the first report of a dental pulp-derived cell with such stable odontoblastic characteristics. TGC could be a very useful model for further study on dental pulp cell.

The effect of calcium phosphate composite scaffolds on the osteogenic differentiation of rabbit dental pulp stem cells

“…[27][28][29] Tsujigiwa et al 30 reported that transplanted rat dental pulp cell in SCID mice with b-TCP particles formed dentin-like and pulp-like structure with lining odontoblast. Beta-tricalcium phosphate (b-TCP), produced by Shanghai Bio-lu Biomaterials Co, Ltd., have received great attention as grafts for the reparation and reconstruction of bone, cartilage and other tissues, as well as for filling bone defects due to its excellent biocompatibility and osteoconduction.…”

“…This kind of β‐TCP bioceramics using as bone regeneration scaffolds since fully interoperability globular pore structure allows tissue infiltration and exhibits good osteoconductive capability . Tsujigiwa et al reported that transplanted rat dental pulp cell in SCID mice with β‐TCP particles formed dentin‐like and pulp‐like structure with lining odontoblast. Zheng et al proved that porcine deciduous pulp stem/progenitor cells mixed with β‐TCP significantly regenerated the dentin‐like structures and nearly completely restored the pulp chamber roof defects.…”

Today Prospects for Tissue Engineering Therapeutic Approach in Dentistry

“…Animal DPSCs have also been tested in several in vivo experiments for regenerative endodontics [ 41 – 46 ]. DPSCs might have possible application in bone regenerative procedures.…”

Preparation of Absorption-Resistant Hard Tissue Using Dental Pulp-Derived Cells and Honeycomb Tricalcium Phosphate

“…Many bone marrow-derived cells are also present in the dental pulp, and dental pulp stem cells have been confirmed to also differentiate into hard tissue-forming cells. These cells also have the potential for use in bone tissue repair [ 13 ].…”