Establishment of Plantlet Regeneration System from Nodal, Internodal and Leaf Explants of Sauropus androgynus (Sweet Shoot)

Abstract

Propagation of Sauropus androgynus (SA) by tissue culture technology provides a better alternative than conventional propagation, as it ensures uniformity in production of sustainable plantlets with desirable traits. The objective was to establish an efficient plant regeneration protocol using leaf, nodal and internodal explants of SA, starting from the establishment of aseptic cultures to the acclimatization stage of plantlets. The effects of plant growth regulators on the growth performance of SA were studied. Results showed in vitro mass multiplication of SA was achieved via indirect organogenesis and shoot regeneration pathway. After 30 days of culture inoculation, high percentage of aseptic cultures were established from nodal (85%), internodal (85%) and leaf (91%) explants sterilized with 70% (v/v) ethanol for 1 min followed by 20% (v/v) Clorox for 20 min. MS medium supplemented with 2.0 mg LG 1 BAP (6-benzylaminopurine) and 0.5 mg LG 1 IAA (indole-3-acetic acid) proved to be effective in inducing adventitious shoots from internodal (95%), nodal (96%) and leaf (90%) explants, after 60 days of culture. High frequency of callus formation (38%) was obtained from leaf explants cultured on MS medium containing 2.0 mg LG 1 IAA and 0.5 mg LG 1 BAP. Rooting response of 90% was achieved from cultured shoots using half-strength MS medium enriched with 1.0 mg LG 1 IAA and from these, 85% survived acclimatization and grew vigorously after 4 weeks. This optimized protocol established a complete in vitro plant regeneration of SA and has never been reported previously. This may form the basis for future research in germplasm conservation and genetic transformation for sustainable production of high quality SA.

Influence of growth regulators on the development, quality, and physiological state of in vitro-propagated Lamprocapnos spectabilis (L.) Fukuhara

“…The present research confirmed the applicability of nodal segments in the in vitro studies with bleeding heart, as all of them demonstrated some type of morphogenetic response, such as caulogenesis, rhizogenesis, callogenesis, and/or embryogenesis. The suitability of this explant type was also reported by We et al (2015), with Sauropus androgynus (L.) Merr. In their research, nodal segments produced a comparable number of adventitious shoots (7.8) as internodes (5.5) and leaf segments (10.0), in the same culture conditions.…”

Characterization of Green Pigment from Sauropus androgynus Shoot Cultures

“…Mean comparison showed an average growth index of shoots significantly varied with BA 1 Kn 0.1 (M3) (Table 1). The higher concentration of BA promotes the development of shoot cultures and increases the number of shoots [25,26]. Green pigment estimation was done in the fresh green leaves samples and shoot cultures of S. androgynus in BA 1 Kn 0.1 medium extracted with methanol.…”