Establishment of pregnancies after serial dilution or direct transfer by vitrified equine embryos

Eldridge-Panuska, W.D.; Brienza, V. Caracciolo di; Seidel, G. E.; Squires, E.L.; Carnevale, E.M.

doi:10.1016/j.theriogenology.2004.06.015

Cited by 70 publications

(54 citation statements)

References 26 publications

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“…Flushing on Day 6 is supposed to reduce the embryo recovery rate (Squires et al 1985, Ferreira et al 1997, Castanheira et al 2004). However, there are also reports on high success rates for embryo collection on Day 6 (Hochi et al 1995, Carnevale et al 2004, Eldridge-Panuska et al 2005. Induction of ovulation was shown to improve the recovery of small embryos ).…”

Section: Discussionmentioning

confidence: 99%

“…Loaded straws are placed into nitrogen vapour for one minute and then plunged into liquid nitrogen. Loading the embryo into a straw allows to transfer the embryo directly after thawing to recipient mares (Carnevale et al 2004) providing a good method for field embryo transfer resulting in good pregnancy rates for morulae and blastocysts smaller than 300µm (Eldridge-Panuska et al 2005). …”

Section: Vitrificationmentioning

confidence: 99%

“…Vitrification of large embryos has not been successful (Carnevale et al 2004, Eldridge-Panuska et al 2005. Large equine embryos had the lowest viability rate in vitro and the highest fracture rate after thawing (Hochi et al 1995).…”

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confidence: 99%

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Cryopreservation of equine embryos

Handler¹,

Neuhauser²

2010

PHK

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Section: Discussionmentioning

confidence: 99%

Section: Vitrificationmentioning

confidence: 99%

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Cryopreservation of equine embryos

Handler¹,

Neuhauser²

2010

PHK

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“…In a practical context, the capsule is implicated in the low success of embryo splitting in the horse and in the failure of expanded blastocysts to be successfully cryopreserved, despite decades of work in this area (Slade et al 1985, Maclellan et al 2002, Eldridge-Panuska et al 2005, Barfield et al 2009). Notably, although embryo biopsy for preimplantation genetic diagnosis (PGD) has been performed in humans since the early 1990's (review, Ogilvie et al (2005)) and has an application for detection and elimination of inherited genetic errors in the horse, this procedure has not been established in equine embryos.…”

Section: Introductionmentioning

confidence: 99%

Viability of equine embryos after puncture of the capsule and biopsy for preimplantation genetic diagnosis

Choi¹,

Gustafson-Seabury²,

Velez³

et al. 2010

Reproduction

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The equine embryo possesses a capsule that is considered essential for its survival. We assessed viability after breaching the capsule of early (Day 6) and expanded (Day 7 and 8) equine blastocysts by micromanipulation. The capsule was penetrated using a Piezo drill, and trophoblast biopsy samples were obtained for genetic analysis. Pregnancy rates for Day-6 embryos, which had intact zonae pellucidae at the time of recovery, were 3/3 for those biopsied immediately after recovery and 2/3 for those biopsied after being shipped overnight under warm (w28 8C) conditions. The pregnancy rates for encapsulated Day-7 expanded blastocysts were 5/6 for those biopsied immediately and 5/6 for those biopsied after being shipped overnight warm. Two of four encapsulated Day-8 blastocysts, 790 and 1350 mm in diameter, established normal pregnancies after biopsy. Nine mares were allowed to maintain pregnancy, and they gave birth to nine normal foals. Biopsied cells from eight embryos that produced foals were subjected to whole-genome amplification. Sex was successfully determined from amplified DNA in 8/8 embryos. Identification of disease-causing mutations matched in the analyses of 6/6 samples for the sodium channel, voltage-gated, type IV, alpha subunit (SCN4A) gene and in 6/7 samples for the peptidylprolyl isomerase B (PPIB) gene, in embryo-foal pairs. Thus, the capsule of the equine embryo can be breached without impairing viability. Further work is needed to determine whether this breach is transient or permanent. These findings are relevant to the understanding of equine embryo development and to the establishment of methods for micromanipulation and embryo cryopreservation in this species.

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“…Meanwhile, to simplify the thawing and transferring procedure and make it more suitable for field use, some researchers have examined the feasibility of in-straw rehydration and direct transfer in the bovine [7], rabbit [8], ovine [9,10] and equine [11]. One feature of this thawing procedure is in-straw dilution, embryo thawing, and direct transfer of the thawed embryos.…”

mentioning

confidence: 99%

Stepwise In-straw Dilution and Direct Transfer Using Open Pulled Straws (OPS) in the Mouse: A Potential Model for Field Manipulation of Vitrified Embryos

Yang

Hou

Zhou

et al. 2007

Journal of Reproduction and Development

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Abstract. In the present study, mouse blastocysts were employed to investigate the feasibility and efficiency of stepwise in-straw dilution and direct transfer using the open pulled straw (OPS) method. In experiment I, the effects of various vitrification solutions (VS) on embryo survival were examined. After thawing, the expanded blastocyst rates (97.59 and 95.05%) and hatching rates (80. 48 In the experiment II, the effects of the volume of VS in the OPS on the survival of embryos after in-straw thawing were investigated. When the length of the VS in the column was less than 1 cm, the in vitro viability of embryos thawed by stepwise in-straw dilution was no different among the experimental and control groups. The embryos could be successfully thawed by immersing the OPS in 0.5 M sucrose for 3 min and then 0.25M sucrose for 2 min. In experiment III, the effect of immersion time of the OPS in diluent (PBS) on the viability of vitrified embryos was investigated. After in-straw thawing, OPSs were immersed immediately in 1 ml PBS for 0 to 30 min. When the immersion time of the OPSs in PBS was less than 12 min, in vitro development of the in-straw thawed embryos was no different from that of the controls. In experiment IV, in-straw thawed blastocysts were directly transferred to pseudopregnant mice to examine their in vivo developmental viability. The pregnancy (91.67%) and birth rates (42.42%) of embryos in-straw thawed and directly transferred were no different from those of the unvitrified controls (90.90 and 40%) and embryos thawed by the conventional method (84.61 and 46.94%). These results demonstrate that mouse embryos vitrified with OPS could be successfully thawed by stepwise in-straw dilution and transferred directly to a recipient and that this method might be a model for field manipulation of vitrified embryos in farm animals. Key words: Blastocyst, In-straw dilution, Mouse, Open pulled straw (OPS), Vitrification (J. Reprod. Dev. 53: [211][212][213][214][215][216][217][218] 2007) itrification, an efficient approach to cryopreservation, has been applied in laboratories to mammalian embryos [1], the oocytes and embryos of domestic animals [2], and the oocytes and

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Establishment of pregnancies after serial dilution or direct transfer by vitrified equine embryos

Cited by 70 publications

References 26 publications

Cryopreservation of equine embryos

Cryopreservation of equine embryos

Viability of equine embryos after puncture of the capsule and biopsy for preimplantation genetic diagnosis

Stepwise In-straw Dilution and Direct Transfer Using Open Pulled Straws (OPS) in the Mouse: A Potential Model for Field Manipulation of Vitrified Embryos

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