Establishment of rapidly proliferating rice cell suspension culture and its characterization by fluorescence-activated cell sorting analysis

Lee, Tae-Jin; Shultz, Randall W.; Hanley‐Bowdoin, Linda; Thompson, William F.

doi:10.1007/bf02773136

Cited by 22 publications

(14 citation statements)

References 30 publications

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“…The flasks were placed on a rotary shaker set at 120 rpm at 25 ± 2°C in the dark. Sub-culturing was undertaken every 3-4 days and the volume of culture adjusted to 75 ml until a homogeneous cell suspension was obtained (Modified from Lee et al (2004)). …”

Section: Establishment Of Callus and Cell Suspension Culturesmentioning

confidence: 99%

Proline biosynthetic gene expression in tissue cultures of rice (Oryza sativa L.) in response to saline treatment

Somboonwatthanaku

Dorling

Leung

et al. 2010

Plant Cell Tiss Organ Cult

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The regulation of proline biosynthesis has been examined in callus and cell cultures of the indica-type rice cultivar Khao Dawk Mali 105 (KDML105) in response to a saline treatment (250 mM NaCl) in terms of the expression of D 1 -pyrroline-5-carboxylate reductase (OsP5CR) and members of the gene family encoding the rate-determining enzyme, D 1 -pyrroline-5-carboxylate synthase (designated OsP5CS1 and OsP5CS2). Using friable callus, growth was retarded by treatment with 250 mM NaCl within 4 days, with a significant increase in the expression of OsP5CS2 by 24 h, and a less marked induction in OsP5CS1 and OsP5CR over the same time-course. Cell suspension cultures derived from the friable callus were also treated with 250 mM NaCl and an induction in OsP5CS2 was again observed, although this was not as marked as in the friable callus, and there was no significant change in OsP5CS1 and OsP5CR expression. This is the first report that details the expression of OsP5CS1 and OsP5CS2 in tissue culture and the results show that, in common with whole plants, OsP5CS2 displays a primacy of response to saline treatment. However, this response may require a community of communicating cells, as occurs in callus tissue, rather than cell suspension cultures. This difference has implications both in terms of the biology of signaling in response to increased salinity and in the use of tissue culture to screen for saline-tolerant germplasm.

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Section: Establishment Of Callus and Cell Suspension Culturesmentioning

confidence: 99%

Proline biosynthetic gene expression in tissue cultures of rice (Oryza sativa L.) in response to saline treatment

Somboonwatthanaku

Dorling

Leung

et al. 2010

Plant Cell Tiss Organ Cult

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“…Nipponbare (Lee et al 2004) were used for isolation of protoplasts. The suspension culture was maintained in the dark and weekly sub-cultured at a dilution of 1:5 (cells: fresh medium).…”

Section: Plant Materials and Culture Conditionsmentioning

confidence: 99%

Transcriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice

et al. 2011

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TitleTranscriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice. Abstract Efficient and cost-effective conversion of plant biomass to usable forms of energy requires a thorough understanding of cell wall biosynthesis, modification and degradation. To elucidate these processes, we assessed the expression dynamics during enzymatic removal and regeneration of rice cell walls in suspension cells over time. In total, 928 genes exhibited significant up-regulation during cell wall removal, whereas, 79 genes were up-regulated during cell wall regeneration. Both gene sets are enriched for kinases, transcription factors and genes predicted to be involved in cell wall-related functions. Integration of the gene expression datasets with a catalog of known and/or predicted biochemical pathways from rice, revealed metabolic and hormonal pathways involved in cell wall degradation and regeneration. Rice lines carrying Tos17 mutations in genes up-regulated during cell wall removal exhibit dwarf phenotypes. Many of the genes up-regulated during cell wall development are also up-regulated in response to infection and environmental perturbations indicating a coordinated response to diverse types of stress.

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“…A number of plant species have been used for generation and propagation of cell-suspension cultures, ranging from model systems like Taxus (Jha et al 1998), Arabidopsis (Richard et al 2001) and Catharanthus (Kim et al 2004) to important monocotyledon or dicotyledon crop plants like soybean (Chu & Lark 1976), alfalfa (Atanassov & Brown 1984), rice (Lee et al 2004), and tobacco (Hellwig et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Detection of anthraquinones and identification of 1,4-naphthohydroquinone in cell suspension cultures of Rudgea jasminoides (Cham.) Müll. Arg. (Rubiaceae)

et al. 2007

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-(Detection of anthraquinones and identification of 1,4-naphthohydroquinone in cell suspension cultures of Rudgea jasminoides (Cham.) Müll. Arg. (Rubiaceae)). In Rubiaceae, anthraquinones and naphthoquinones are secondary metabolites characteristic of the subfamily Rubioideae, in which Rudgea jasminoides is included. Thin-layer chromatography using specific solvent systems and spray reagents indicated the presence of anthraquinones constitutively produced by cell suspension cultures of R. jasminoides. GC/MS analysis detected 1,4-naphthohydroquinone as a product of biosynthesis only after elicitation of the cells with yeast extract (Saccharomyces cerevisiae). The latter compound is probably a phytoalexin produced by suspension cultures of R. jasminoides.

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Establishment of rapidly proliferating rice cell suspension culture and its characterization by fluorescence-activated cell sorting analysis

Cited by 22 publications

References 30 publications

Proline biosynthetic gene expression in tissue cultures of rice (Oryza sativa L.) in response to saline treatment

Proline biosynthetic gene expression in tissue cultures of rice (Oryza sativa L.) in response to saline treatment

Transcriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice

Detection of anthraquinones and identification of 1,4-naphthohydroquinone in cell suspension cultures of Rudgea jasminoides (Cham.) Müll. Arg. (Rubiaceae)

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