Establishment of six human pancreatic cancer cell lines and their sensitivities to anti-tumor drugs.

Abstract

and -16, were established. They originated from either primary pancreatic cancer biopsy or liver metastasis biopsy, or xenografts of these biopsy specimens in athymic nude mice. The primary tumors were all well differentiated adenocarcinoma of pancreatic duct origin. The six established PK cell lines were all CEA positive and had tumorigenicity in athymic nude mice. Morphology of the xenografted tumors was closely similar to that of the original tumor. PK cells grew slowly with the doubling time of 41.3 to 82 hr and showed aneuploid chromosome pattern. High levels of glucose-6-phosphate dehydrogenase (G6PDH) and lactic dehydrogenase (LDH) were found in each cell extract. Trypsin was not detected in cell extracts except PK-8 and PK-9. In chemosensitivity test, all of PK cell lines were sensitive to aclacinomycin A (ACM), and PK-1 and PK-8 were sensitive to 5-Fluorouracil (5-Fu) at concentrations of 0.02 pg/ml, ACM and l;ug/ml, 5-Fu, when the drugs were used for over 48 hr. At higher concentrations, they showed time independent sensitivity to mitomycin C (MMC). PK-9 was resistant to 5-Fu and MMC. pancreatic cancer cell lines ; xenografted tumors ; chemosensitivity testIn spite of recent advances of diagnostic techniques, it is still difficult to diagnose pancreatic cancer in its early stage. Many chemotherapeutic and radiotherapeutic trials have been tested, but no good results have been obtained yet (Zimmerman et al.

RNA Interference Targeting Aurora Kinase A Suppresses Tumor Growth and Enhances the Taxane Chemosensitivity in Human Pancreatic Cancer Cells

“…Three human pancreatic cancer cell lines, Panc-1, MIA PaCa-2, and SU.86.86, were purchased from American Type Culture Collection (Manassas, VA), and PK-1 was obtained from the original developer (17). All cells were maintained in RPMI 1640 containing 10% fetal bovine serum under atmosphere of 5% CO 2 with humidity at 37jC.…”

Oncolytic replication-competent adenovirus suppresses tumor angiogenesis through preserved E1A region

“…The other cell lines, PK-1 and PK-8, were established in our department. 35 All of these cell lines have mutations in the TP53 gene 36 and were maintained in RPMI 1640 medium containing 10% fetal bovine serum, 2 mmol/l L-glutamine, 50 U/ml penicillin, and 50 mg/ml streptomycin in a humidified 5% CO 2 atmosphere at 371C.…”

Overexpression of the p53-inducible brain-specific angiogenesis inhibitor 1 suppresses efficiently tumour angiogenesis

“…In order to check the expression of BAI1 we used six well-known human pancreatic adenocarcinoma cell lines (Panc-1, MiaPaCa2, AsPC-1, BxPC3, PCI35 and Su.Su.86), three cell lines established in our department (PK-1, PK-8, and PK-9, Kobari et al, 1986), and two widely-known colon cancer cell lines (T-84 and HT-29). Panc-1, MiaPaCa2, and T-84 cells were maintained in DMEM (Gibco, Tokyo, Japan) with glucose, the others in RPMI 1640 (Gibco, Tokyo, Japan), except for HT-29 cells that were cultured in McCoy's 5 A medium (Gibco, Tokyo, Japan).…”