Establishment of Specific Pathogen-Free (SPF) Rat Colonies using Gnotobiotic Techniques.

Yanabe, Makoto; Shibuya, Masaharu; Gonda, Toshihiko; Asai, Hidekazu; Tanaka, Toshio; Sudou, Katuko; Narita, Teruo; Itoh, Kazuyoshi

doi:10.1538/expanim.50.293

Cited by 12 publications

(11 citation statements)

References 14 publications

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“…The rat and mouse intestinal microbiota consists of complex bacterial communities, which has been confirmed by culture methods and 16S rRNA gene clone library analyses (24,37,46). In our FISH analysis, we focused on Bacteroides, Bifidobacterium, Clostridium, Eubacterium, Lactobacillus, and Streptococcus, which have been reported to be the members of the rat and mouse intestinal microbiota (37,38,46).…”

Section: Discussionmentioning

confidence: 70%

“…In our FISH analysis, we focused on Bacteroides, Bifidobacterium, Clostridium, Eubacterium, Lactobacillus, and Streptococcus, which have been reported to be the members of the rat and mouse intestinal microbiota (37,38,46). Six sets of genus-or group-specific oligonucleotide probes identified about 29 to 55% of the DAPI-stained cells and about 32 to 64% of the active bacterial cells (Eub338) in the rat cecal communities ( Table 3).…”

Section: Discussionmentioning

confidence: 99%

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Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve

Dinoto

Suksomcheep

Ishizuka

et al. 2006

Appl Environ Microbiol

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To investigate the effects of administration of raffinose and encapsulated Bifidobacterium breve JCM 1192 T cells on the rat cecal microbiota, in a preclinical synbiotic study groups of male WKAH/Hkm Slc rats were fed for 3 weeks with four different test diets: basal diet (group BD), basal diet supplemented with raffinose (group RAF), basal diet supplemented with encapsulated B. breve (group CB), and basal diet supplemented with both raffinose and encapsulated B. breve (group RCB). The bacterial populations in cecal samples were determined by fluorescence in situ hybridization (FISH) and terminal restriction fragment length polymorphism (T-RFLP). B. breve cells were detected only in the RCB group and accounted for about 6.3% of the total cells as determined by FISH analysis. B. breve was also detected only in the RCB group by T-RFLP analysis. This was in contrast to the CB group, in which no B. breve signals were detected by either FISH or T-RFLP. Increases in the sizes of the populations of Bifidobacterium animalis, a Bifidobacterium indigenous to the rat, were observed in the RAF and RCB groups. Principalcomponent analysis of T-RFLP results revealed significant alterations in the bacterial populations of rats in the RAF and RCB groups; the population in the CB group was similar to that in the control group (group BD). To the best of our knowledge, these results provide the first clear picture of the changes in the rat cecal microbiota in response to synbiotic administration.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve

Dinoto

Suksomcheep

Ishizuka

et al. 2006

Appl Environ Microbiol

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“…Experimental animals are generally maintained under specific pathogen-free (SPF) conditions. 15) However, SPF animals are raised from gnotobiotic animals, which harbor defined (or semi-defined) intestinal microflora. 15) Each breeder might have his own bacterial cocktail for the creation of gnotobiotic animals.…”

Section: Clostridiiformes (M59089)mentioning

confidence: 99%

“…15) However, SPF animals are raised from gnotobiotic animals, which harbor defined (or semi-defined) intestinal microflora. 15) Each breeder might have his own bacterial cocktail for the creation of gnotobiotic animals. Therefore, the composition of intestinal bacteria produced by different breeders is expected to vary.…”

Section: Clostridiiformes (M59089)mentioning

confidence: 99%

The Composition of Intestinal Bacteria Affects the Level of Luminal IgA

Ohashi

Hiraguchi

Ushida

2006

Bioscience, Biotechnology, and Biochemistry

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“…The hindgut microbiota of commercially available rats are artificially constructed and are so simple that they often cause abnormal fer mentation, which is characterized by hyperlactate pro duction in the hindgut when indigestible oligosaccha rides are fed (24,26). They usually lack the predomi nant acid-utilizing groups of bacteria (24,27,28). Experiments with pigs may have advantages over those with rats in this context because typical commercial pigs do not have artificially controlled simple intestinal microbiota; therefore, an examination of indigestible oligosaccharides can be conducted under conventional conditions.…”

mentioning

confidence: 99%

Stimulation of Butyrate Production in the Large Intestine of Weaning Piglets by Dietary Fructooligosaccharides and Its Influence on the Histological Variables of the Large Intestinal Mucosa

Tsukahara

Iwasaki²,

Nakayama³

et al. 2003

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryFructooligosaccharides (FOS) reach the large intestine and are fermented into short-chain fatty acids (SCFA), lactate, and carbon dioxide. As the major energy source for the epithelial cells of the large intestine, n-butyrate stimulates the proliferation of cells as well as mineral and water absorption from the lumen. We examined the effect of dietary FOS supplementation on luminal SCFA production and its influence on the morphometrical vari ables of mucosa of the large intestine in commercially available pigs. Six weaning piglets were used. After 7 d of adaptation, three pigs were given a test diet containing FOS (10%) ad libitum for 10 d. The other three remained on the basal diet and were used as controls . At the end of the experiment, their large intestines were removed, and the cecum, gyri centrip etales, gyri centrifugales, and rectum were separated. The contents of each portion were col lected and measured for SCFA concentration, pH, and moisture. A micrometer was used to measure the crypt depth. The numbers of epithelial and mitotic cells in the crypt columns were also counted. The concentration of SCFA was significantly higher in piglets fed FOS than in the controls. The concentration of n-butyrate was markedly stimulated by POS . The number of epithelial, mitotic, and mucin-containing cells was higher in piglets fed FOS than in the controls. Accordingly, the crypt depth was larger in the FOS-fed piglets. The luminal n-butyrate concentration showed a significantly positive correlation with the crypt depth and the number of epithelial, mitotic, and mucin-containing cells.

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Establishment of Specific Pathogen-Free (SPF) Rat Colonies using Gnotobiotic Techniques.

Cited by 12 publications

References 14 publications

Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve

Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve

The Composition of Intestinal Bacteria Affects the Level of Luminal IgA

Stimulation of Butyrate Production in the Large Intestine of Weaning Piglets by Dietary Fructooligosaccharides and Its Influence on the Histological Variables of the Large Intestinal Mucosa

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