Establishment of suspension culture condition for embryogenic callus proliferation and somatic embryo development of Kalopanax septemlobus

Kyu, Moon Heung; 김선자,

doi:10.5010/jpb.2009.36.1.007

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…Callus of K. septemlobus was induced as described by Kim and Moon (2009) using a callus induction medium containing Murashige and Skoog (MS) solid media supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 3% sucrose, and 0.7% plant agar. All cultures were kept in a dark condition at 24 °C.…”

Section: Plant Materials and Culture Conditionsmentioning

confidence: 99%

Enhanced biosynthesis of saponins by coronatine in cell suspension culture of Kalopanax septemlobus

2018

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is a medicinal woody species of the family Araliaceae, and the pharmaceutical properties of saponins obtained from . suggest that . has the potential to be a crude drug and dietary health supplement. In this study, we established cell suspension culture of . to develop a sustainable source of natura-ceuticals. Friable calli were used for establishing cell suspension culture. The maximum amount of total saponins (1.56 mg/60 ml suspension) was obtained during the 15th day of incubation, whereas the maximum capacity of saponin production was reached after day 6 (0.42 μg/mg of fresh weight). The total saponin production in the cell suspension of . was significantly increased by coronatine (COR) at 160% at a dose of 1 μM compared with the mock-treated control, whereas methyl jasmonate treated cells exhibited less increase in total saponin level as compared to the COR-treated cells. In addition, the elicitation of COR strongly induced the expression of beta-amyrin synthase, thus resulting in the accumulation of oleanolic acid (2.369 ± 0.98 μg/mg of extract), a precursor for oleanane-type triterpene saponins. These results indicate that COR is an efficient elicitor for inducing phytochemicals in cell suspension culture and that it provides the possibility for producing saponins of . using cell suspension culture.

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Section: Plant Materials and Culture Conditionsmentioning

confidence: 99%

Enhanced biosynthesis of saponins by coronatine in cell suspension culture of Kalopanax septemlobus

2018

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“…대개 습기가 많고 부엽이 많은 사 면 (斜面)에서 자라며 작은 군락을 이루지만 그 개체수는 적 다 (Lee and Lee, 1997). 체세포배 발생 (somatic embyogenesis)을 이용한 식물체생 산기술은 식물체의 대량생산에 있어 다른 배양방법보다 효율 적인 번식법으로 인식되고 있고, 두릅나무과의 수종 중 가시 오갈피와 음나무의 조직배양 연구가 보고된 바 있으며 (Choi et al, 1999;Moon et al, 2008), 대량배양에 대한 연구 또 한 활발히 이루어져 음나무와 두릅나무에서 배발생 캘러스의 현탁배양을 통한 체세포배 발달 및 식물체 재분화에 대한 연 구가 시도된바 있다 (Kim and Moon, 2009;Jhang et al, 및 체세포배 배양기술이 어느 정도 확립되었으나 (Moon et al, 2006) 엽기배로의 발달이 억제된 상태로 동조화된 것을 확인할 수 있었다 (Table 5)…”

Section: 서 언unclassified

“…. 두릅나무 (Aralia elata S.)의 경우, 고체배지에서의 2,4-D와 thidiazuron를 조합하여 캘러스를 유기하는 단계, 1/4 MS 배지 에서 두릅나무의 체세포배를 유도와 발아를 유도하는 단계로 나누어진 연구가 이루어졌으며(Jhang et al, 1994), 두릅나무 과에 속하는 음나무 (Kalopanax septemlobus K.)의 연구에서 는 액체배양을 통한 배발생 캘러스의 증식 및 체세포배를 효 과적으로 유도하고 체세포배 생산을 위한 배양 기간의 단축이 가능함을 보여주었다(Kim and Moon, 2009). 이전 연구에서는 땃두릅나무의 체세포배 형성과 유식물체 유도를 위하여 0.3% gelrite를 교질물질로 사용한 고체배지 상 에서 2,4-D와 thidiazuron를 조합 처리하여 캘러스를 유기하는 단계, 이를 ABA와 활성탄을 함유한 배지에 옮겨 체세포배를 유기하는 단계, gibberellic acid를 처리하여 체세포배의 발아를 유도하는 단계로 3단계로 나누어 진행하였다 (Moon et al, 2006).…”

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Establishment of Suspension Culture System to Induce Somatic Embryo in Oplopanax elatus Nakai

Kim¹,

Seong²,

Lee³

et al. 2012

Korean Journal of Medicinal Crop Science

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: This study was carried out to select the appropriate medium (especially, carbon and nitrogen source, potassium phosphate and pH) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of Oplopanax elatus Nakai. Direct somatic embryos were obtained from root explants in the hormone free suspension culture (MS). Combination of NH 4 NO 3 and KNO 3 at the ratio of 1650 (㎎/ℓ ) : 1900 (㎎/ℓ ) obtained the better result to produce somatic embryo in suspension culture. MS medium supplemented with 170 ㎎/ℓ KH 2 PO 4 . The addition of 1 and 3% sucrose was effective for formation of embryogenic callus. Therefore, this report will be helped to improve the establishment for suspension culture in Oplopanax elatus Nakai.

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“…이 같은 결과는 Park et al (1993)이 Mentha viridis의 캘러스를 저온저장 시 갈변화 정도가 저 장 기간에 비례하고 온도에 있어서도 5°C보다 10°C에서 갈변화의 진행정도가 더 빠르게 진행되었다는 결과와 유 사하였다. Kim et al (2009)은 세포의 갈변화는 세포의 활 력감소를 의미한다고 하였는데 본 실험에서 저온저장 기 간이 길어지면서 세포의 갈변화가 진행되고 이로 인해 SE 유도빈도가 저조해진 것으로 생각된다 (Fig. 5B-1, C-1…”

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Somatic embryo induction and plant regeneration from cold-stored embryogenic callus ofK. septemlobus

2015

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Somatic embryogenesis is as an excellent technology for potential use in plant mass production, germplasm conservation, or genetic engineering. We examined the effect of cold storage using 3 embryogenic callus lines with different levels of embryogenesis competence derived from immature zygotic embryo cultures of Kalopanax setemlobus. Somatic embryo induction, germination and plant conversion were evaluated after 1, 3 and 6 months storage at 4°C in the dark. Most cold-stored embryogenic calli formed somatic embryos normally even after 6 months; however, the induction rate was gradually decreased by increasing the storage period. The most competent line tended to show a slight decline in somatic embryo induction rate, as compared with other lines after cold storage. In general, cold storage resulted in reduced somatic embryo germination and plant regeneration, although 93% somatic embryo germination and 91% plant conversion were achieved regardless of the storage period. Cold storage led to cell browning and degradation. Additionally, the cell structures were confirmed by the aceto-carmine and evans blue dye evaluation. Collectively, our results showed that embryogenic callus of K. septemlobus could be preserved at 4°C without subculture for 6 months, and suggested the need for storage of relatively more competent embryogenic calli lines to support somatic embryo induction.

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Establishment of suspension culture condition for embryogenic callus proliferation and somatic embryo development of Kalopanax septemlobus

Cited by 5 publications

References 31 publications

Enhanced biosynthesis of saponins by coronatine in cell suspension culture of Kalopanax septemlobus

Enhanced biosynthesis of saponins by coronatine in cell suspension culture of Kalopanax septemlobus

Establishment of Suspension Culture System to Induce Somatic Embryo in Oplopanax elatus Nakai

Somatic embryo induction and plant regeneration from cold-stored embryogenic callus ofK. septemlobus

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