Esterase-17 (ES-17): Characterization and genetic location on chromosome 9 of a bis-p-nitrophenyl phosphate-resistant esterase of the house mouse (Mus musculus)

Abstract

Genetic variation of a new codominantly inherited esterase, designated ES-17, has been discovered in the house mouse using isoelectric focusing in polyacrylamide gels. The ES-17 A phenotype (three bands; isoelectric points, between pH 5.55 and pH 5.90) was found in C57BL/10Sn. LP/J possessed the Es-17B phenotype (three bands; isoelectric points, pH 5.05-5.55). ES-17 was present in all tissues examined, except for hemolysate and serum, and was most clearly expressed in the small intestine. Because of its reaction toward various substrates and inhibitors, ES-17 has tentatively been classified as acetyl esterase (EC 3.1.1.6). ES-17 was shown to be controlled by the structural locus Es-17, located on chromosome 9. From test-cross data, a gene order of Es-17-8.7 +/- 2.5 map units-Mpi-1-10.2 +/- 2.7 map units-Mod-1 was established.

Purification and properties of esterase-4 from Drosophila mojavensis

“…The preference of esterase-4 for naphthyl propionate does not define the class of enzyme it belongs to, because of the usually very broad substrate specificities of esterases. But esterases may be classified using inhibitors like those used in this study (Table III) [16,22]. Inhibition experiments on esterases run on non-denaturing gels are often used to classify esterases [22][23][24].…”

“…But esterases may be classified using inhibitors like those used in this study (Table III) [16,22]. Inhibition experiments on esterases run on non-denaturing gels are often used to classify esterases [22][23][24]. The low sensitivity of esterase-4 to diisopropyl fluorophosphate, eserine and p-chloromercuribenzoate (Table III), suggests that esterase-4 is an acetyl esterase (EC 3.1.1.6).…”

Συμβολη Στη Μελετη Του Αμφιβιου Mertensiella Luschani (Steindachner 1891) (Urodela Salamandridae). Πληθυσμιακα Και Βιοχημικα Στοιχεια

“…Οι εστεράσες θεωρούνται σαν ένα από τα συνθετώτερα και πλέον εύπλαστα ισοενζυμικά συστήματα. Κατά τους Otto and von Deimling (1983), von , von , Radler (1986), Coates and Cortner (1986) (Augustinsson .1960, Holmes et al 1968…”