Esterase 2-oligodeoxynucleotide conjugates as sensitive reporter for electrochemical detection of nucleic acid hybridization

Positive and Negative Regulation of Vertebrate Separase by Cdk1-Cyclin B1 May Explain Why Securin Is Dispensable

“…The enzyme substrate p-aminophenyl butyrate was delivered through the flow chamber at a flow rate of 250 l/min. After the flow was stopped, the esterase 2 activity was measured as a change in current intensity (dI) per time (dt) within the first 5 s (22,23).…”

“…Electrochemical Biochip Analysis of mRNA Levels-Electrochemical biochip analysis of mRNA levels was performed as described previously (22,23). Capture oligonucleotides were as follows: securin, ACGGTTCCCGAAGGCACATTCTCATTTT-TTTTT (3Ј-thiol); and separase, TCTGCCCCCGAAGGGGAC-GTCCTATTTTTTTTT (3Ј-thiol).…”

Detection of bacterial 16S rRNA using multivalent dendrimer-reporter enzyme conjugates

“…Monomeric polypeptide chain of esterase 2 is suitable for covalent and site-directed chemical modifications and conjugations (Humenik et al, , 2008Wang et al, 2007). Thermostability of esterase 2 and its covalent binding to detector ODN allows onestep hybridization at elevated temperatures.…”

“…Flow of pAPB was stopped after the current reached a steady state and slope of the current was measured to assay the EST2 enzymatic activity. It was assumed that this parameter is proportional to the amount of analyte RNA (Nebling et al, 2004;Wang et al, 2007).…”

Electrochemical Detection of miRNAs

“…One relevant example is the use of the thermostable reporter enzyme esterase 2 (ETS2) from Alicyclobacillus acidocaldarius. [37] EST2 offers the possibility of site specific modification with oligonucleotide probes and was found to be superior to common enzyme labels such as alkaline phosphatase and horseradish peroxidase (HRP) [38].…”