Esterification of n-protected-tyrosine by alpha-chymotrypsin in a high concentration of ethanol

Theobaldo, F. C.; Lira, Elisabeth; Cheng, Elisabeth; Irokawa, Ayako; Tominaga, Mineko

doi:10.1007/bf00152760

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1993

1998

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Synthesis of L-tyrosine glyceryl ester catalyzed by α-chymotrypsin in water-miscible organic solvents: A possible sun-tan accelerator product

et al. 1993

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The synthesis of L-tyrosine glyceryl ester, from glycerol and L-tyrosine methyl ester, was carried out by a transesterification reaction catalyzed by a-chymotrypsin. Values of 60 % (v/v) for glycerol and 200 mM for L-tyrosine methyl ester were optimal for the transesterification reaction. Additionally to glycerol, several other water miscible cosolvents (acetonitrile, N,N'-dime@ formamide and tetrahydrofurane) were tested in the reaction media, but their presence did not give an enhancement on the transesterification activity with respect to the glycerol/water medium. However, increasing the hydrophobic@ of the cosolvent resulted in a reduction of the enzyme activity, the water:glycerol mixture being the best reaction media.

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