Estimación de la diversidad genética de Anadara tuberculosa en cinco manglares de Tumaco, utilizando la enzima citocromo oxidasa I

Abstract

Objective. To estimate the genetic diversity of the Anadara tuberculosa en five mangrove swams of Tumaco, Nariño, Colombia using as a mitocondrial molecular marker the cytochromo oxidase sub-unit I (COI). Materials and methods. A total of 50 individuals were collected from the San Jorge, La Tiburonera, El Pajal, La Playa y Bajito Vaquería mangrove swamps, randomly selecting 10 specimens of each zone. The tissue sample was worked with absolute alcohol at ambient temperature in microtubes. DNA was extracted, and the mitocondrial DNA was amplified using the PCR technique (polymerase chain reaction). The amplified and quantified products of PCR were sequenced on both sides (Macrogen). Each one of the obtained sequences was edited and aligned. Later, the parameters of genetic diversity (haplotypical and nucleotidical) were measured, and the analysis of distribution between frequency pairs (Mistmach distribution) was elaborated. Finally, the analysis of nucleotidic variation and population structure (AMOVA) was completed. Results. The amplified product gene weighed 710 bp. The haplotypical diversity reported for all the populations was high (0.683±0.060) and the reported nucleotídical diversity was low for all the populations (0.040±0.020). The AMOVA results indicate that the variance amongst populations is low (4.20%) and that the variance within populations is high (95.80%). Conclusions. The studied populations are not structured and although there is a decrease of natural banks, the genetic diversity is high.

Production of specific pathogen free larvae from genetically characterized populations of Anadara tuberculosa (Bivalvia), for stock enhancement and aquaculture in the Peru Northeast Biosphere Reserve

“…The star-like shape of the TCS network and the shallow length of branches (the maximum distance between the ancestor haplotype and the most divergent haplotype corresponds to only seven nucleotides) would suggest a recent population expansion (Clement et al, 2000). As a point of comparison, haplotype diversity of A. tuberculosa from the NSMT was higher (36.7 vs 28.5%) than the genetic diversity reported for the same species extracted from Nariño region (Colombia) by Chamorro & Rosero (2016) or from Anadara granosa from Malaysia (14.7%) (Chee et al, 2011b). Figure 2.…”