In contrast to the extensive research on the propagation evolution of internal solitary waves (ISWs) over continental shelf topography, few investigations have been conducted on the propagation evolution of ISWs over downslope topography. In this experiment, the characteristics of the ISWs passing over a downslope topography were investigated in an immiscible two-layer fluid system to clearly capture the waveform information. The main objective of this study was to analyze the propagation evolution of ISWs with different incident amplitudes passing through a downslope topography. Using the particle image velocimetry technique, the evolutionary characteristics of ISWs (such as waveform, wave height, energy budget, and flow field) are investigated. According to the flow field analysis results, the phase velocity near the downslope topography changes with the propagation of ISWs. Moreover, the gradient distribution of velocity progressively becomes perpendicular to the right surface of the topography. Furthermore, a counterclockwise vortex gradually forms at the right upper vertex of the topography. The propagation of ISWs is influenced by varying water depth and topography. The front waveform of ISWs is significantly flat, and the back is steeper than the original back waveform. Around the tail wave, an upward convex wave packet is generated. The trends of wave energy and wave height losses are broadly consistent. Furthermore, the energy loss is related to the incident wave height with a 20% higher loss rate for large-amplitude ISWs than small-amplitude ISWs at the inflection point of the topography.